Isolation and Characterization of Nine Microsatellite Loci from the Hawaiian Grouper Epinephelus Quernus (Serranidae) for Population Genetic Analyses View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-04

AUTHORS

Malia Ana J. Rivera, Glenn C. Graham, George K. Roderick

ABSTRACT

The availability of variable genetic markers for groupers (Serranidae) has generally been limited to mitochondrial DNA. For studies of population genetic structure, more loci are usually required; particularly useful are those that are nuclear in origin such as microsatellites. Here, we isolated and characterized 9 microsatellite loci from the endemic Hawaiian grouper Epinephelus quernus using a biotin-labeled oligonucleotide-streptavidin–coated magnetic bead approach. Of the 20 repeat-containing fragments isolated, 15 had sufficient flanking region in which to design primers. Among these, 9 produced consistent polymerase chain reaction product, and 6 were highly variable. These 6 loci were all composed of dinucleotide repeats, with the number of alleles ranging from 6 to 18, and heterozygosities from 33.3% to 91.7%. The high levels of variability observed should make these markers useful for population genetic studies of E. quernus, and potentially other epinephelines. More... »

PAGES

126-129

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10126-002-0093-y

DOI

http://dx.doi.org/10.1007/s10126-002-0093-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022824401

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12876647


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