A methodological approach to investigate steady state fucoxanthin chlorophyll a/c binding protein mRNA levels in Wadden Sea sediments View Full Text


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Article Info

DATE

2003-03

AUTHORS

Telse Meyer, Michael Hust, Jürgen Marquardt, Wolfgang E. Krumbein, Erhard Rhiel

ABSTRACT

A method was established to investigate the steady state levels of mRNAs from genes encoding fucoxanthin chlorophyll a/c binding proteins (Fcp) of diatoms in situ. During the study, which was performed with Wadden Sea sediments from the German North Sea shore near Dangast, oxygenic photosynthesis was carried out mainly by pennate diatoms. Field samples were taken after tidal exposure from dawn up to late afternoon at 2-hourly intervals, and frozen in liquid nitrogen. In the laboratory, total RNA was isolated by isopycnic ultracentrifugation in caesium chloride gradients. Yields of approximately 10-300 micro g RNA per gram wet sediment were obtained. Defined amounts of total RNA were blotted onto nylon membranes and hybridised with probes against the fcp2 and 18S rDNA genes of Cyclotella cryptica. To estimate the steady state amount of fcp mRNAs, fcp signal intensities were normalized to the signal intensities obtained from hybridisation to an 18S rDNA gene probe. In the two time-course studies performed to demonstrate the applicability of the method, the steady state levels of fcp mRNA increased up to 12-fold with the onset of light, reaching a maximum 6-8 h after sunrise before they decreased again. Possible reasons for this time-course are discussed. More... »

PAGES

33-39

References to SciGraph publications

  • 1995-07. The gene family encoding the fucoxanthin chlorophyll proteins from the brown alga Macrocystis pyrifera in MOLECULAR GENETICS AND GENOMICS
  • 1996-12. The fucoxanthin-chlorophyll proteins from a chromophyte alga are part of a large multigene family: structural and evolutionary relationships to other light harvesting antennae in MOLECULAR GENETICS AND GENOMICS
  • 1995-10. Molecular study of a light-harvesting apoprotein of Giraudyopsis stellifer (Chrysophyceae) in PLANT MOLECULAR BIOLOGY
  • 1969-09. Silicoborate als erste nicht C-haltige Wachstumsfaktoren in ARCHIVES OF MICROBIOLOGY
  • 1989-12. Molecular characterization of the diurnal/circadian expression of the chlorophyll a/b-binding proteins in leaves of tomato and other dicotyledonous and monocotyledonous plant species in PLANTA
  • 1998-12. The genes encoding light-harvesting subunits of Cyclotella cryptica (Bacillariophyceae) constitute a complex and heterogeneous family in MOLECULAR GENETICS AND GENOMICS
  • 1999-01. A Phylogenetic Assessment of the Eukaryotic Light-Harvesting Antenna Proteins, with Implications for Plastid Evolution in JOURNAL OF MOLECULAR EVOLUTION
  • 1995-02. Nucleotide sequence of two cDNAs encoding fucoxanthin chlorophyll a/c proteins in the diatom Odontella sinensis in PLANT MOLECULAR BIOLOGY
  • 1990-10. Light-harvesting proteins of diatoms: Their relationship to the chlorophyll a/b binding proteins of higher plants and their mode of transport into plastids in MOLECULAR GENETICS AND GENOMICS
  • 1959-09. Studies with cyanidium caldarium, an anomalously pigmented chlorophyte in ARCHIVES OF MICROBIOLOGY
  • 1999-08. Semi-quantitative RT-PCR analysis of photoregulated gene expression in marine diatoms in PLANT MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10123-003-0098-z

    DOI

    http://dx.doi.org/10.1007/s10123-003-0098-z

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12730710


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