Effects of 915 nm GaAs diode laser on mitochondria of human dermal fibroblasts: analysis with confocal microscopy View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-10-29

AUTHORS

Silvana Belletti, Jacopo Uggeri, Giovanni Mergoni, Paolo Vescovi, Elisabetta Merigo, Carlo Fornaini, Samir Nammour, Maddalena Manfredi, Rita Gatti

ABSTRACT

Low-level laser therapy (LLLT) is widely used in tissue regeneration and pain therapy. Mitochondria are supposed to be one of the main cellular targets, due to the presence of cytochrome C oxidase as photo-acceptor. Laser stimulation could influence mitochondria metabolism affecting mainly transmembrane mitochondrial potential (Δψm). The aim of our study is to evaluate “in vitro” the early mitochondrial response after irradiation with a 915 GaAs laser. Since some evidences suggest that cellular response to LLLT can be differently modulated by the mode of irradiation, we would like to evaluate whether there are changes in the mitochondrial potential linked to the use of the laser treatments applied with continuous wave (CW) in respect to those applied with pulsed wave (PW). In this study, we analyzed effects of irradiation with a 915-nm GaAs diode laser on human dermal fibroblast. We compared effects of irradiation applied with either CW or PW at different fluences 45-15-5 J/cm2 on Δψm. Laser scanning microscopy (LSM) was used in living cells to detect ROS (reactive oxygen species) using calcein AM and real-time changes of and Δψm following distribution of the potentiometric probe tetramethylrhodamine methyl ester (TMRM). At higher doses (45–15 J/cm2), fibroblasts showed a dose-dependent decrement of Δψm in either the modalities employed, with higher amplitudes in CW-treated cells. This behavior is transient and not followed by any sign of toxicity, even if reactive oxygen species generation was observed. At 5 J/cm2, CW irradiation determined a little decrease (5 %) of the baseline level of Δψm, while opposite behavior was shown when cells were irradiated with PW, with a 10 % increment. Our results suggest that different responses observed at cellular level with low doses of irradiation, could be at the basis of efficacy of LLLT in clinical application, performed with PW rather than CW modalities. More... »

PAGES

375-381

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10103-014-1651-z

DOI

http://dx.doi.org/10.1007/s10103-014-1651-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048037588

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/25351448


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79 laser
80 laser scanning microscopy
81 laser stimulation
82 laser therapy
83 laser treatment
84 levels
85 little decrease
86 low doses
87 low-level laser therapy
88 main cellular target
89 metabolism
90 methyl ester
91 microscopy
92 mitochondria
93 mitochondria metabolism
94 mitochondrial potential
95 mitochondrial response
96 modalities
97 mode
98 mode of irradiation
99 opposite behavior
100 oxidase
101 oxygen species generation
102 pain therapy
103 potential
104 potentiometric probe tetramethylrhodamine methyl ester
105 presence
106 probe tetramethylrhodamine methyl ester
107 reactive oxygen species generation
108 real-time changes
109 regeneration
110 respect
111 response
112 results
113 scanning microscopy
114 signs
115 signs of toxicity
116 species generation
117 stimulation
118 study
119 target
120 tetramethylrhodamine methyl ester
121 therapy
122 tissue regeneration
123 toxicity
124 transmembrane mitochondrial potential
125 treatment
126 use
127 waves
128 ΔΨm
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