Enzymatic hydrolysis of thermochemically pretreated biomass using a mixture of cellulolytic enzymes produced from different fungal sources View Full Text


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Article Info

DATE

2017-02-25

AUTHORS

Shivani Sharma, Arindam Kuila, Vinay Sharma

ABSTRACT

Enzymatic hydrolysis of pretreated lignocellulosic raw material is one of the major steps in the biofuel production. In this study, sorghum straw was pretreated under previously optimized conditions (using 0.5 M sodium hydroxide, 8% substrate concentration, 120 °C temperature and 20 min of incubation time). Cellulase enzymes were produced using three different fungal sources (Aspergillus niger, Fusarium oxysporum and Trichoderma harzianum). Enzymatic hydrolysis of pretreated biomass was carried out using individual and different mixture of cellulases. Maximum reducing sugar yield was obtained when an equal mixture of three different cellulases was used. The enzymatic hydrolysis process was optimized through central composite design based on response surface methodology using an equal mixture of three different cellulases (1:1:1). The data showed that maximum reducing sugar yield of 464.2 mg/g dry substrate was obtained at 10% substrate concentration, 55 °C and after 48 h of incubation. Further, batch (a process where all the substrates were fed into the bioreactor at the beginning of the reaction) and fed-batch (a process where substrates were fed into the bioreactor at specific time intervals) process of enzymatic hydrolysis were compared at high substrate concentration (20%). The results showed that an increase in reducing sugar yield (355.6 mg/g dry substrate) was obtained in case of fed-batch enzymatic hydrolysis as compared to batch enzymatic hydrolysis (285.3 mg/g dry substrate) process. The above results can be useful for efficient hydrolysis of lignocellulosic biomass and cost-effective biofuel production. More... »

PAGES

1577-1584

References to SciGraph publications

  • 2009-06-08. Yield-determining factors in high-solids enzymatic hydrolysis of lignocellulose in BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
  • 2013-09-09. Novel perspectives for evolving enzyme cocktails for lignocellulose hydrolysis in biorefineries in SUSTAINABLE CHEMICAL PROCESSES
  • 2016-09-01. A β-glucosidase hyper-production Trichoderma reesei mutant reveals a potential role of cel3D in cellulase production in MICROBIAL CELL FACTORIES
  • 2013-01-28. Understanding of alkaline pretreatment parameters for corn stover enzymatic saccharification in BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
  • 2014-02-17. Process optimization and production kinetics for cellulase production by Trichoderma viride VKF3 in SPRINGERPLUS
  • 2016-06-21. Cellulase production using natural medium and its application on enzymatic hydrolysis of thermo chemically pretreated biomass in 3 BIOTECH
  • 2013-07-04. Comparative efficiency of different pretreatment methods on enzymatic digestibility of Parthenium sp. in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 2012-03-20. Kinetic study of batch and fed-batch enzymatic saccharification of pretreated substrate and subsequent fermentation to ethanol in BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
  • 2014-04-26. Formulation of enzyme blends to maximize the hydrolysis of alkaline peroxide pretreated alfalfa hay and barley straw by rumen enzymes and commercial cellulases in BMC BIOTECHNOLOGY
  • 2016-10-01. Optimization of Dilute Acid Pretreatment and Enzymatic Hydrolysis of Phalaris aquatica L. Lignocellulosic Biomass in Batch and Fed-Batch Processes in BIOENERGY RESEARCH
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    http://scigraph.springernature.com/pub.10.1007/s10098-017-1346-9

    DOI

    http://dx.doi.org/10.1007/s10098-017-1346-9

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