Determination of HCV Genotype Using Two Antibody Assays and Genome Typing View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-09

AUTHORS

K. Sandres, M. Dubois, C. Pasquier, J. Puel, J. Izopet

ABSTRACT

Two serotyping assays for hepatitis C virus (Serotyping 1-6 assay; Murex, UK and RIBA Serotyping SI; Chiron, USA) were compared to a standardized genotyping assay (Inno-LiPA HCV II; Innogenetics, Belgium) using serum samples collected from 126 patients chronically infected with hepatitis C virus. Serotyping was positive in 87% and 80% of the samples tested with Murex and Chiron, respectively, and concordant with genotyping in 93% and 88%, respectively. Sequence analysis of the NS5b region of 15 samples with discrepant typing results confirmed the Inno-LiPA finding in all instances. The Murex enzyme immunoassay serotyping method was less sensitive for identifying genotype 2 (P<0.05). The concordance with genotyping of the RIBA serotyping method was lower for genotype 2 than for the other genotypes (P<0.05). More... »

PAGES

666-669

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s100960100576

DOI

http://dx.doi.org/10.1007/s100960100576

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1018724844

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11714052


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