Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2021-02-16

AUTHORS

Norihito Kaku, Kohji Hashiguchi, Norihiko Akamatsu, Fumiko Wakigawa, Junichi Matsuda, Kenzo Komaru, Takumi Nakao, Yosuke Harada, Atsuko Hara, Naoki Uno, Kei Sakamoto, Yoshitomo Morinaga, Takeshi Kitazaki, Hiroo Hasegawa, Taiga Miyazaki, Masaaki Fukuda, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara

ABSTRACT

We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses. More... »

PAGES

1743-1748

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10096-021-04193-7

DOI

http://dx.doi.org/10.1007/s10096-021-04193-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1135406717

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33594599


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