A protein microarray immunoassay for the serological evaluation of the antibody response in vertically transmitted infections View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-09

AUTHORS

A. Ardizzoni, B. Capuccini, M. C. Baschieri, C. F. Orsi, F. Rumpianesi, S. Peppoloni, C. Cermelli, M. Meacci, A. Crisanti, P. Steensgaard, E. Blasi

ABSTRACT

The detection of specific serum antibodies is mainly achieved by enzyme-linked immunosorbent assay (ELISA). Here, we describe the setting up of a microarray-based serological assay to screen for IgG and IgM against vertically transmitted pathogens (Toxoplasma gondii, rubella virus, cytomegalovirus, herpes simplex virus types 1 and 2, varicella zoster virus, Chlamydia trachomatis). The test, accommodated onto a restricted area of a microscope slide, consists of: (a) the immobilization of antigens and human IgG and IgM antibody dilution curves, laid down in an orderly manner; (b) addition of serum samples; (c) detection of antigen-serum antibodies complexes by indirect immunofluorescence. The IgG and IgM curves provide an internal calibration system for the interpolation of the signals from the single antigens. The test was optimized in terms of spotting conditions and processing protocol. The detection limit was 400 fg for the IgG assay and 40 fg for the IgM assay; the analytical specificity was >98%. The clinical sensitivity returned an average value of 78%, the clinical specificity was >96%, the predictive values were >73%, and the efficiency was >88%. The results obtained make this test a promising tool, suitable for introduction in the clinical diagnostic routine of vertically transmitted infections, in parallel (and in future as an alternative) to ELISA. More... »

PAGES

1067-1075

References to SciGraph publications

  • 2008-12. The epidemiology of Varicella Zoster Virus infection in Italy in BMC PUBLIC HEALTH
  • 1988-02. Prevalence of herpes simplex virus type 2 antibodies in selected population groups in Italy in EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
  • 2004-04-05. Protein Arrays for Serodiagnosis of Disease in PROTEIN ARRAYS
  • 2003-08. Screening for infectious diseases in CHILD'S NERVOUS SYSTEM
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s10096-009-0748-y

    DOI

    http://dx.doi.org/10.1007/s10096-009-0748-y

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1012838810

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19415353


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    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10096-009-0748-y'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10096-009-0748-y'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10096-009-0748-y'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10096-009-0748-y'


     

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