Gene transfer in skeletal muscle by systemic injection of DODAC lipopolyplexes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-12

AUTHORS

F. Pampinella, M. Pozzobon, E. Zanetti, P.G. Gamba, I. McLachlan, M. Cantini, I. Vitiello

ABSTRACT

Lipid-based vectors are a promising tool for gene therapy applications. Several studies have reported their use in vivo to transfect different organs. Few data, however, are available about lipid-mediated gene transfer in skeletal muscle. Here we report the initial results obtained after systemic administration of lipopolyplexes based on the DODAC cationic lipid in an animal model of muscle regeneration. In particular, we compared three routes of administration: intravenous (IV), intracardiac (IC) and intra-arterial (IA). Analysis of reporter gene expression (luciferase) showed that regenerating muscle is more efficiently transfected in all cases and that IA injection is by far the best approach. More... »

PAGES

s967-s969

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s100720070011

DOI

http://dx.doi.org/10.1007/s100720070011

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1043297925

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11382197


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