Novel in vitro culture condition improves the stemness of human dermal stem/progenitor cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2013-12

AUTHORS

Joong Hyun Shim, Tae Ryong Lee, Dong Wook Shin

ABSTRACT

Cell therapy using adult stem cells has emerged as a potentially new approach for the treatment of various diseases. Therefore, it is an essential procedure to maintain the stemness of adult stem cells for clinical treatment. We previously reported that human dermal stem/progenitor cells (hDSPCs) can be enriched using collagen type IV. However, hDSPCs gradually lose their stem cell properties as in vitro passages continue. In the present study, we developed optimized in vitro culture condition to improve the stemness of these hDSPCs. To evaluate whether the stemness of hDSPCs is well sustained in various culture conditions, we measured the expression levels of SOX2, NANOG, and S100B, which are well-known representative dermal progenitor markers. We observed that hDSPCs grown in three-dimensional (3D) culture condition had higher expression levels of those markers compared with hDSPCs grown in two-dimensional (2D) culture condition. Under the 3D culture condition, we further demonstrated that a high glucose (4.5 g/L) concentration enhanced the expression levels of the dermal progenitor markers, whereas O(2) concentration did not affect. We also found that skin-derived precursor (SKP) culture medium was the most effective, among various culture media, in increasing the dermal progenitor marker expression. We finally demonstrated that this optimized culture condition enhanced the expression level of human telomerase reverse transcriptase (hTERT), the proliferation, and the multipotency of hDSPCs, an important characteristic of stem cells. Taken together, these results suggested that this novel in vitro culture condition improves the stemness of hDSPCs. More... »

PAGES

556-563

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10059-013-0260-1

DOI

http://dx.doi.org/10.1007/s10059-013-0260-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030823007

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24241684


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1004", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Biotechnology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/10", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Technology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adult", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adult Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomarkers", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Culture Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Proliferation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Collagen Type IV", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dermis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucose", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Homeodomain Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Multipotent Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nanog Homeobox Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oxygen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "S100 Calcium Binding Protein beta Subunit", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "SOXB1 Transcription Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Telomerase", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Bioscience Research Institute, Amorepacific Corporation R&D Center, 446-729, Yongin, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shim", 
        "givenName": "Joong Hyun", 
        "id": "sg:person.01311324773.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311324773.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Bioscience Research Institute, Amorepacific Corporation R&D Center, 446-729, Yongin, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Tae Ryong", 
        "id": "sg:person.01037657027.77", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037657027.77"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Bioscience Research Institute, Amorepacific Corporation R&D Center, 446-729, Yongin, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shin", 
        "givenName": "Dong Wook", 
        "id": "sg:person.07434135732.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07434135732.79"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.92.11.4857", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008119461"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature09603", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011608783", 
          "https://doi.org/10.1038/nature09603"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jdermsci.2012.06.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015020372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1634/stemcells.2004-0134", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015653454"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1634/stemcells.2004-0134", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015653454"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.cellbio.22.010305.104357", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016155037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb0901-778", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018972963", 
          "https://doi.org/10.1038/ncb0901-778"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb0901-778", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018972963", 
          "https://doi.org/10.1038/ncb0901-778"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2007.05.054", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019257253"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10059-011-2176-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019945845", 
          "https://doi.org/10.1007/s10059-011-2176-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci26043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020026671"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/jid.2011.428", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021352881", 
          "https://doi.org/10.1038/jid.2011.428"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12015-009-9094-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022192173", 
          "https://doi.org/10.1007/s12015-009-9094-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12015-009-9094-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022192173", 
          "https://doi.org/10.1007/s12015-009-9094-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0911647107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024097304"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2009.10.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026291523"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2006.05.169", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030371881"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0301-472x(02)00869-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034217844"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0501283102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037397831"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00870", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040325841", 
          "https://doi.org/10.1038/nature00870"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature00870", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040325841", 
          "https://doi.org/10.1038/nature00870"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bcp.2008.09.033", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040349457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/emm.2007.71", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041042141", 
          "https://doi.org/10.1038/emm.2007.71"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/emm.2007.71", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041042141", 
          "https://doi.org/10.1038/emm.2007.71"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.284.5411.143", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042379237"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/stem.420", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042451096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.stem.2009.05.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042574725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/71630", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042917021", 
          "https://doi.org/10.1038/71630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/71630", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042917021", 
          "https://doi.org/10.1038/71630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0508440102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043730332"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tins.2005.08.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045218625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1242/dev.038620", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046209824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0050742", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047233113"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.0030331", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047602245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.0030331", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047602245"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1091/mbc.e02-02-0105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048841479"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1181", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050220442", 
          "https://doi.org/10.1038/ncb1181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/ncb1181", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050220442", 
          "https://doi.org/10.1038/ncb1181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.279.5349.349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051728512"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1359/jbmr.070725", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053403470"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/107632701300062859", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059205960"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/scd.2012.0244", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059310620"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/scd.2013.0067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059310797"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4137/bmi.s1173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077940234"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4137/bmi.s1173", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077940234"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082571780", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013-12", 
    "datePublishedReg": "2013-12-01", 
    "description": "Cell therapy using adult stem cells has emerged as a potentially new approach for the treatment of various diseases. Therefore, it is an essential procedure to maintain the stemness of adult stem cells for clinical treatment. We previously reported that human dermal stem/progenitor cells (hDSPCs) can be enriched using collagen type IV. However, hDSPCs gradually lose their stem cell properties as in vitro passages continue. In the present study, we developed optimized in vitro culture condition to improve the stemness of these hDSPCs. To evaluate whether the stemness of hDSPCs is well sustained in various culture conditions, we measured the expression levels of SOX2, NANOG, and S100B, which are well-known representative dermal progenitor markers. We observed that hDSPCs grown in three-dimensional (3D) culture condition had higher expression levels of those markers compared with hDSPCs grown in two-dimensional (2D) culture condition. Under the 3D culture condition, we further demonstrated that a high glucose (4.5 g/L) concentration enhanced the expression levels of the dermal progenitor markers, whereas O(2) concentration did not affect. We also found that skin-derived precursor (SKP) culture medium was the most effective, among various culture media, in increasing the dermal progenitor marker expression. We finally demonstrated that this optimized culture condition enhanced the expression level of human telomerase reverse transcriptase (hTERT), the proliferation, and the multipotency of hDSPCs, an important characteristic of stem cells. Taken together, these results suggested that this novel in vitro culture condition improves the stemness of hDSPCs.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10059-013-0260-1", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1115754", 
        "issn": [
          "1016-8478", 
          "0219-1032"
        ], 
        "name": "Molecules and Cells", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "36"
      }
    ], 
    "name": "Novel in vitro culture condition improves the stemness of human dermal stem/progenitor cells", 
    "pagination": "556-563", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "92d687ff803bd988b9966875a012c13da369b093d99b2424f65da4ece3d35b10"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24241684"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9610936"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10059-013-0260-1"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1030823007"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10059-013-0260-1", 
      "https://app.dimensions.ai/details/publication/pub.1030823007"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T14:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8660_00000481.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s10059-013-0260-1"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10059-013-0260-1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10059-013-0260-1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10059-013-0260-1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10059-013-0260-1'


 

This table displays all metadata directly associated to this object as RDF triples.

277 TRIPLES      21 PREDICATES      84 URIs      39 LITERALS      27 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10059-013-0260-1 schema:about N13a961697aba4567a4b67409091c3c8a
2 N2f778bac02af4927820c7342c2cde138
3 N5ce3dbe97ad54310b7be1f94f7706827
4 N602b32c6a4474a11a7e789075c1bbe16
5 N75802092136c44e2be1cefad8dc66ba0
6 N817fdd1590eb4e429e3f6d51c5d7bfb5
7 N8226fc9a0a5b458c994fb0c9ca67ea70
8 N824674ec49684a37be3e05bbc1434f53
9 N855092c0bf9844df859b95a792aaaf45
10 N8c339fca3fb345a0af2bb8b4a8a981e3
11 N99c9045b78fd480082c35a6cd9304dde
12 Nb76fab590e94442ba48c57f7e454a512
13 Nbb1411dff78f43d9aeb85b9f0055bcad
14 Ndf79af6913c24cc992ba1246e81031a5
15 Ne093616963794a19ba307999c859f013
16 Nea46d482a28a4a3a98466db789802c76
17 Nfce8d1b9051a48cd9acbb2db6537ce46
18 Nfe8c5a826c3a4740a6fca040cc25aab6
19 anzsrc-for:10
20 anzsrc-for:1004
21 schema:author N5e778e3575d84078b36f0b48b1a46f2d
22 schema:citation sg:pub.10.1007/s10059-011-2176-y
23 sg:pub.10.1007/s12015-009-9094-9
24 sg:pub.10.1038/71630
25 sg:pub.10.1038/emm.2007.71
26 sg:pub.10.1038/jid.2011.428
27 sg:pub.10.1038/nature00870
28 sg:pub.10.1038/nature09603
29 sg:pub.10.1038/ncb0901-778
30 sg:pub.10.1038/ncb1181
31 https://app.dimensions.ai/details/publication/pub.1082571780
32 https://doi.org/10.1002/stem.420
33 https://doi.org/10.1016/j.bbrc.2006.05.169
34 https://doi.org/10.1016/j.bbrc.2007.05.054
35 https://doi.org/10.1016/j.bcp.2008.09.033
36 https://doi.org/10.1016/j.jdermsci.2012.06.007
37 https://doi.org/10.1016/j.stem.2009.05.001
38 https://doi.org/10.1016/j.stem.2009.10.019
39 https://doi.org/10.1016/j.tins.2005.08.008
40 https://doi.org/10.1016/s0301-472x(02)00869-x
41 https://doi.org/10.1073/pnas.0501283102
42 https://doi.org/10.1073/pnas.0508440102
43 https://doi.org/10.1073/pnas.0911647107
44 https://doi.org/10.1073/pnas.92.11.4857
45 https://doi.org/10.1089/107632701300062859
46 https://doi.org/10.1089/scd.2012.0244
47 https://doi.org/10.1089/scd.2013.0067
48 https://doi.org/10.1091/mbc.e02-02-0105
49 https://doi.org/10.1126/science.279.5349.349
50 https://doi.org/10.1126/science.284.5411.143
51 https://doi.org/10.1146/annurev.cellbio.22.010305.104357
52 https://doi.org/10.1172/jci26043
53 https://doi.org/10.1242/dev.038620
54 https://doi.org/10.1359/jbmr.070725
55 https://doi.org/10.1371/journal.pbio.0030331
56 https://doi.org/10.1371/journal.pone.0050742
57 https://doi.org/10.1634/stemcells.2004-0134
58 https://doi.org/10.4137/bmi.s1173
59 schema:datePublished 2013-12
60 schema:datePublishedReg 2013-12-01
61 schema:description Cell therapy using adult stem cells has emerged as a potentially new approach for the treatment of various diseases. Therefore, it is an essential procedure to maintain the stemness of adult stem cells for clinical treatment. We previously reported that human dermal stem/progenitor cells (hDSPCs) can be enriched using collagen type IV. However, hDSPCs gradually lose their stem cell properties as in vitro passages continue. In the present study, we developed optimized in vitro culture condition to improve the stemness of these hDSPCs. To evaluate whether the stemness of hDSPCs is well sustained in various culture conditions, we measured the expression levels of SOX2, NANOG, and S100B, which are well-known representative dermal progenitor markers. We observed that hDSPCs grown in three-dimensional (3D) culture condition had higher expression levels of those markers compared with hDSPCs grown in two-dimensional (2D) culture condition. Under the 3D culture condition, we further demonstrated that a high glucose (4.5 g/L) concentration enhanced the expression levels of the dermal progenitor markers, whereas O(2) concentration did not affect. We also found that skin-derived precursor (SKP) culture medium was the most effective, among various culture media, in increasing the dermal progenitor marker expression. We finally demonstrated that this optimized culture condition enhanced the expression level of human telomerase reverse transcriptase (hTERT), the proliferation, and the multipotency of hDSPCs, an important characteristic of stem cells. Taken together, these results suggested that this novel in vitro culture condition improves the stemness of hDSPCs.
62 schema:genre research_article
63 schema:inLanguage en
64 schema:isAccessibleForFree true
65 schema:isPartOf N7a9d36dc893645e1b595ea3536abb84e
66 Nc16b3bc31eaf442eb094496e6ea1cc47
67 sg:journal.1115754
68 schema:name Novel in vitro culture condition improves the stemness of human dermal stem/progenitor cells
69 schema:pagination 556-563
70 schema:productId N0dc36372002d4868928a7275f7ec1374
71 N234081da90ae4fd3b080ef78f4e41b80
72 N502bd791bfae41fdb1a91bd3c9b4de1d
73 N886b5a1ef7f84ef2baf3e25223b46396
74 N97340a52ed2d4664bc91029bae2001b7
75 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030823007
76 https://doi.org/10.1007/s10059-013-0260-1
77 schema:sdDatePublished 2019-04-10T14:01
78 schema:sdLicense https://scigraph.springernature.com/explorer/license/
79 schema:sdPublisher Nb4ab9410a61746c6bb8f159803a59a8c
80 schema:url http://link.springer.com/10.1007/s10059-013-0260-1
81 sgo:license sg:explorer/license/
82 sgo:sdDataset articles
83 rdf:type schema:ScholarlyArticle
84 N066dc5f0abd14bea9126d8efff5e2f23 schema:name Bioscience Research Institute, Amorepacific Corporation R&D Center, 446-729, Yongin, Korea
85 rdf:type schema:Organization
86 N0dc36372002d4868928a7275f7ec1374 schema:name pubmed_id
87 schema:value 24241684
88 rdf:type schema:PropertyValue
89 N13a961697aba4567a4b67409091c3c8a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Multipotent Stem Cells
91 rdf:type schema:DefinedTerm
92 N234081da90ae4fd3b080ef78f4e41b80 schema:name readcube_id
93 schema:value 92d687ff803bd988b9966875a012c13da369b093d99b2424f65da4ece3d35b10
94 rdf:type schema:PropertyValue
95 N253fa7c5e6d84185935a32b2342543ea rdf:first sg:person.07434135732.79
96 rdf:rest rdf:nil
97 N2f778bac02af4927820c7342c2cde138 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Dermis
99 rdf:type schema:DefinedTerm
100 N502bd791bfae41fdb1a91bd3c9b4de1d schema:name doi
101 schema:value 10.1007/s10059-013-0260-1
102 rdf:type schema:PropertyValue
103 N5ce3dbe97ad54310b7be1f94f7706827 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Nanog Homeobox Protein
105 rdf:type schema:DefinedTerm
106 N5e778e3575d84078b36f0b48b1a46f2d rdf:first sg:person.01311324773.63
107 rdf:rest N61214dcbf3e842b3bb22497f946cab68
108 N602b32c6a4474a11a7e789075c1bbe16 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Cell Differentiation
110 rdf:type schema:DefinedTerm
111 N61214dcbf3e842b3bb22497f946cab68 rdf:first sg:person.01037657027.77
112 rdf:rest N253fa7c5e6d84185935a32b2342543ea
113 N75802092136c44e2be1cefad8dc66ba0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Adult
115 rdf:type schema:DefinedTerm
116 N7a9d36dc893645e1b595ea3536abb84e schema:volumeNumber 36
117 rdf:type schema:PublicationVolume
118 N817fdd1590eb4e429e3f6d51c5d7bfb5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Oxygen
120 rdf:type schema:DefinedTerm
121 N8226fc9a0a5b458c994fb0c9ca67ea70 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name S100 Calcium Binding Protein beta Subunit
123 rdf:type schema:DefinedTerm
124 N824674ec49684a37be3e05bbc1434f53 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name SOXB1 Transcription Factors
126 rdf:type schema:DefinedTerm
127 N855092c0bf9844df859b95a792aaaf45 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Adult Stem Cells
129 rdf:type schema:DefinedTerm
130 N886b5a1ef7f84ef2baf3e25223b46396 schema:name nlm_unique_id
131 schema:value 9610936
132 rdf:type schema:PropertyValue
133 N8c339fca3fb345a0af2bb8b4a8a981e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Humans
135 rdf:type schema:DefinedTerm
136 N97340a52ed2d4664bc91029bae2001b7 schema:name dimensions_id
137 schema:value pub.1030823007
138 rdf:type schema:PropertyValue
139 N99c9045b78fd480082c35a6cd9304dde schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Biomarkers
141 rdf:type schema:DefinedTerm
142 Nb4ab9410a61746c6bb8f159803a59a8c schema:name Springer Nature - SN SciGraph project
143 rdf:type schema:Organization
144 Nb76fab590e94442ba48c57f7e454a512 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Homeodomain Proteins
146 rdf:type schema:DefinedTerm
147 Nbb1411dff78f43d9aeb85b9f0055bcad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Glucose
149 rdf:type schema:DefinedTerm
150 Nc16b3bc31eaf442eb094496e6ea1cc47 schema:issueNumber 6
151 rdf:type schema:PublicationIssue
152 Nd62fee699fcd4d9095baed4f51371017 schema:name Bioscience Research Institute, Amorepacific Corporation R&D Center, 446-729, Yongin, Korea
153 rdf:type schema:Organization
154 Nda94805f818745c7858baaadf38e7157 schema:name Bioscience Research Institute, Amorepacific Corporation R&D Center, 446-729, Yongin, Korea
155 rdf:type schema:Organization
156 Ndf79af6913c24cc992ba1246e81031a5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Telomerase
158 rdf:type schema:DefinedTerm
159 Ne093616963794a19ba307999c859f013 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Cells, Cultured
161 rdf:type schema:DefinedTerm
162 Nea46d482a28a4a3a98466db789802c76 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Collagen Type IV
164 rdf:type schema:DefinedTerm
165 Nfce8d1b9051a48cd9acbb2db6537ce46 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
166 schema:name Cell Culture Techniques
167 rdf:type schema:DefinedTerm
168 Nfe8c5a826c3a4740a6fca040cc25aab6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Cell Proliferation
170 rdf:type schema:DefinedTerm
171 anzsrc-for:10 schema:inDefinedTermSet anzsrc-for:
172 schema:name Technology
173 rdf:type schema:DefinedTerm
174 anzsrc-for:1004 schema:inDefinedTermSet anzsrc-for:
175 schema:name Medical Biotechnology
176 rdf:type schema:DefinedTerm
177 sg:journal.1115754 schema:issn 0219-1032
178 1016-8478
179 schema:name Molecules and Cells
180 rdf:type schema:Periodical
181 sg:person.01037657027.77 schema:affiliation Nd62fee699fcd4d9095baed4f51371017
182 schema:familyName Lee
183 schema:givenName Tae Ryong
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01037657027.77
185 rdf:type schema:Person
186 sg:person.01311324773.63 schema:affiliation N066dc5f0abd14bea9126d8efff5e2f23
187 schema:familyName Shim
188 schema:givenName Joong Hyun
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311324773.63
190 rdf:type schema:Person
191 sg:person.07434135732.79 schema:affiliation Nda94805f818745c7858baaadf38e7157
192 schema:familyName Shin
193 schema:givenName Dong Wook
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07434135732.79
195 rdf:type schema:Person
196 sg:pub.10.1007/s10059-011-2176-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1019945845
197 https://doi.org/10.1007/s10059-011-2176-y
198 rdf:type schema:CreativeWork
199 sg:pub.10.1007/s12015-009-9094-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022192173
200 https://doi.org/10.1007/s12015-009-9094-9
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/71630 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042917021
203 https://doi.org/10.1038/71630
204 rdf:type schema:CreativeWork
205 sg:pub.10.1038/emm.2007.71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041042141
206 https://doi.org/10.1038/emm.2007.71
207 rdf:type schema:CreativeWork
208 sg:pub.10.1038/jid.2011.428 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021352881
209 https://doi.org/10.1038/jid.2011.428
210 rdf:type schema:CreativeWork
211 sg:pub.10.1038/nature00870 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040325841
212 https://doi.org/10.1038/nature00870
213 rdf:type schema:CreativeWork
214 sg:pub.10.1038/nature09603 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011608783
215 https://doi.org/10.1038/nature09603
216 rdf:type schema:CreativeWork
217 sg:pub.10.1038/ncb0901-778 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018972963
218 https://doi.org/10.1038/ncb0901-778
219 rdf:type schema:CreativeWork
220 sg:pub.10.1038/ncb1181 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050220442
221 https://doi.org/10.1038/ncb1181
222 rdf:type schema:CreativeWork
223 https://app.dimensions.ai/details/publication/pub.1082571780 schema:CreativeWork
224 https://doi.org/10.1002/stem.420 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042451096
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/j.bbrc.2006.05.169 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030371881
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/j.bbrc.2007.05.054 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019257253
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/j.bcp.2008.09.033 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040349457
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/j.jdermsci.2012.06.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015020372
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/j.stem.2009.05.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042574725
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/j.stem.2009.10.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026291523
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/j.tins.2005.08.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045218625
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/s0301-472x(02)00869-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034217844
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1073/pnas.0501283102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037397831
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1073/pnas.0508440102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043730332
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1073/pnas.0911647107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024097304
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1073/pnas.92.11.4857 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008119461
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1089/107632701300062859 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059205960
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1089/scd.2012.0244 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059310620
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1089/scd.2013.0067 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059310797
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1091/mbc.e02-02-0105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048841479
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1126/science.279.5349.349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051728512
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1126/science.284.5411.143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042379237
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1146/annurev.cellbio.22.010305.104357 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016155037
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1172/jci26043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020026671
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1242/dev.038620 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046209824
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1359/jbmr.070725 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053403470
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1371/journal.pbio.0030331 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047602245
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1371/journal.pone.0050742 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047233113
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1634/stemcells.2004-0134 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015653454
275 rdf:type schema:CreativeWork
276 https://doi.org/10.4137/bmi.s1173 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077940234
277 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...