Efficient enhancement of lentiviral transduction efficiency in murine spermatogonial stem cells View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2012-05

AUTHORS

Bang-Jin Kim, Ki-Jung Kim, Yong-Hee Kim, Yong-An Lee, Byung-Gak Kim, Chul Min Cho, Hye-Ryeon Kang, Chul Geun Kim, Buom-Yong Ryu

ABSTRACT

Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis throughout postnatal life in male and have the ability to transmit genetic information to the subsequent generation. In this study, we have optimized the transduction efficiency of SSCs using a lentiviral vector by considering different multiplicity of infection (MOI), duration of infection, presence or absence of feeder layer and polycationic agents. We tested MOI of 5, 10 or 20 and infection duration of 6, 9 or 12 h respectively. After infection, cells were cultured for 1 week and as a result, the number of transduced SSCs increased significantly for MOI of 5 and 10 with 6 h of infection. When the same condition (MOI of 5 with 6 hours) was applied in presence or absence of STO feeder layer and infected SSCs were cultured for 3 weeks on the STO feeder layer, a significant increase in the number of transduced cells was observed for without the feeder layer during infection. We subsequently studied the effects of polycationic agents, polybrene and dioctadecylamidoglycyl spermine (DOGS), on the transduction efficiency. Compared with the polybrene treatment, the recovery rate of the transduced SSCs was significantly higher for the DOGS treatment. Therefore, our optimization study could contribute to the enhancement of germ-line modification of SSCs using lentiviral vectors and in generation of transgenic animals. More... »

PAGES

449-455

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10059-012-2167-7

DOI

http://dx.doi.org/10.1007/s10059-012-2167-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015319171

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22526390


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals, Genetically Modified", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lentivirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice, Inbred C57BL", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spermatogenesis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spermatogonia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stem Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transduction, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science and Technology, Chung-Ang University, 456-756, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Bang-Jin", 
        "id": "sg:person.0706046135.57", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706046135.57"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science and Technology, Chung-Ang University, 456-756, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Ki-Jung", 
        "id": "sg:person.0730322707.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730322707.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science and Technology, Chung-Ang University, 456-756, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Yong-Hee", 
        "id": "sg:person.0602661244.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602661244.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science and Technology, Chung-Ang University, 456-756, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "Yong-An", 
        "id": "sg:person.01326265344.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01326265344.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science and Technology, Chung-Ang University, 456-756, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Byung-Gak", 
        "id": "sg:person.01315223635.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315223635.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "BET Research Institute, Chung-Ang University, 456-756, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cho", 
        "givenName": "Chul Min", 
        "id": "sg:person.0617735407.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617735407.02"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science and Technology, Chung-Ang University, 456-756, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kang", 
        "givenName": "Hye-Ryeon", 
        "id": "sg:person.0734164007.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0734164007.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hanyang University", 
          "id": "https://www.grid.ac/institutes/grid.49606.3d", 
          "name": [
            "Department of Life Science, College of Natural Sciences, Hanyang University, 133-791, Seoul, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kim", 
        "givenName": "Chul Geun", 
        "id": "sg:person.01116635740.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01116635740.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chung-Ang University", 
          "id": "https://www.grid.ac/institutes/grid.254224.7", 
          "name": [
            "Department of Animal Science and Technology, Chung-Ang University, 456-756, Ansung, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ryu", 
        "givenName": "Buom-Yong", 
        "id": "sg:person.01171073502.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171073502.48"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1095/biolreprod.103.017012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002372289"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0396-339", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003259518", 
          "https://doi.org/10.1038/nbt0396-339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod.109.079558", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005725785"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0506970102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008600171"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.97.15.8346", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009438925"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2164/jandrol.106.001511", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015652573"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.111158198", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016710226"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(88)90020-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018961462"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2001-11-0042", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019955154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.84.21.7413", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022874269"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(87)49054-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024313074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300715", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029746234", 
          "https://doi.org/10.1038/sj.gt.3300715"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.gt.3300715", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029746234", 
          "https://doi.org/10.1038/sj.gt.3300715"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(02)03010-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032617529"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ydbio.2004.07.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032636156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ydbio.2003.07.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033050974"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ydbio.2003.07.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033050974"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/335088a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037763215", 
          "https://doi.org/10.1038/335088a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4157(93)90004-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037861864"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4157(93)90004-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037861864"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0631767100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039001089"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1095/biolreprod.103.016519", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041124466"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/24.15.3113", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042490645"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0407063101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046300934"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.24.11298", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047711366"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.222561399", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049443866"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.24.11303", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049656402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.6272397", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062635980"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079436472", 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-68315-2_11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1081602573", 
          "https://doi.org/10.1007/978-3-642-68315-2_11"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082936465", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2012-05", 
    "datePublishedReg": "2012-05-01", 
    "description": "Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis throughout postnatal life in male and have the ability to transmit genetic information to the subsequent generation. In this study, we have optimized the transduction efficiency of SSCs using a lentiviral vector by considering different multiplicity of infection (MOI), duration of infection, presence or absence of feeder layer and polycationic agents. We tested MOI of 5, 10 or 20 and infection duration of 6, 9 or 12 h respectively. After infection, cells were cultured for 1 week and as a result, the number of transduced SSCs increased significantly for MOI of 5 and 10 with 6 h of infection. When the same condition (MOI of 5 with 6 hours) was applied in presence or absence of STO feeder layer and infected SSCs were cultured for 3 weeks on the STO feeder layer, a significant increase in the number of transduced cells was observed for without the feeder layer during infection. We subsequently studied the effects of polycationic agents, polybrene and dioctadecylamidoglycyl spermine (DOGS), on the transduction efficiency. Compared with the polybrene treatment, the recovery rate of the transduced SSCs was significantly higher for the DOGS treatment. Therefore, our optimization study could contribute to the enhancement of germ-line modification of SSCs using lentiviral vectors and in generation of transgenic animals.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s10059-012-2167-7", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1115754", 
        "issn": [
          "1016-8478", 
          "0219-1032"
        ], 
        "name": "Molecules and Cells", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "33"
      }
    ], 
    "name": "Efficient enhancement of lentiviral transduction efficiency in murine spermatogonial stem cells", 
    "pagination": "449-455", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "48fd383855b3fcfc38fa4b1f8b522f34b0995c72d0cd7de2ddb938e49bacc3d7"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "22526390"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9610936"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s10059-012-2167-7"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1015319171"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s10059-012-2167-7", 
      "https://app.dimensions.ai/details/publication/pub.1015319171"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T13:07", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000487.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s10059-012-2167-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s10059-012-2167-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s10059-012-2167-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s10059-012-2167-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s10059-012-2167-7'


 

This table displays all metadata directly associated to this object as RDF triples.

263 TRIPLES      21 PREDICATES      69 URIs      33 LITERALS      21 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s10059-012-2167-7 schema:about N1a7bee21965846549816743928d23d06
2 N1bf98d25f7f44d389a2da3d558c10823
3 N1c19bac40c014b08842c07f42fe04972
4 N4d5543916546439cb6b45f00656996a9
5 N5817e21ee8ef4ca0b62a14e640cde5b4
6 N5912a2962bc94bbe8f0fe9d1fe4402a1
7 N5ad0e6730dc44774be1e4bbb308a159d
8 N8a976a336b2f44709ab134c42a4c8d42
9 N8cc618af47c649a588d8f53866243300
10 N9c951f7b41ca4c6ba875191ed92c1d45
11 Na5aab28c0f11417786c90217b83107fc
12 Nb604214f94a6483e91764057d6a7bdde
13 anzsrc-for:11
14 anzsrc-for:1108
15 schema:author N61f6ed9b8f5d4ee4ba5c8536d09a3f2d
16 schema:citation sg:pub.10.1007/978-3-642-68315-2_11
17 sg:pub.10.1038/335088a0
18 sg:pub.10.1038/nbt0396-339
19 sg:pub.10.1038/sj.gt.3300715
20 https://app.dimensions.ai/details/publication/pub.1079436472
21 https://app.dimensions.ai/details/publication/pub.1082936465
22 https://doi.org/10.1016/0076-6879(87)49054-x
23 https://doi.org/10.1016/0092-8674(88)90020-7
24 https://doi.org/10.1016/0304-4157(93)90004-8
25 https://doi.org/10.1016/j.ydbio.2003.07.010
26 https://doi.org/10.1016/j.ydbio.2004.07.004
27 https://doi.org/10.1016/s0014-5793(02)03010-7
28 https://doi.org/10.1073/pnas.0407063101
29 https://doi.org/10.1073/pnas.0506970102
30 https://doi.org/10.1073/pnas.0631767100
31 https://doi.org/10.1073/pnas.111158198
32 https://doi.org/10.1073/pnas.222561399
33 https://doi.org/10.1073/pnas.84.21.7413
34 https://doi.org/10.1073/pnas.91.24.11298
35 https://doi.org/10.1073/pnas.91.24.11303
36 https://doi.org/10.1073/pnas.97.15.8346
37 https://doi.org/10.1093/nar/24.15.3113
38 https://doi.org/10.1095/biolreprod.103.016519
39 https://doi.org/10.1095/biolreprod.103.017012
40 https://doi.org/10.1095/biolreprod.109.079558
41 https://doi.org/10.1126/science.6272397
42 https://doi.org/10.1182/blood-2001-11-0042
43 https://doi.org/10.2164/jandrol.106.001511
44 schema:datePublished 2012-05
45 schema:datePublishedReg 2012-05-01
46 schema:description Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis throughout postnatal life in male and have the ability to transmit genetic information to the subsequent generation. In this study, we have optimized the transduction efficiency of SSCs using a lentiviral vector by considering different multiplicity of infection (MOI), duration of infection, presence or absence of feeder layer and polycationic agents. We tested MOI of 5, 10 or 20 and infection duration of 6, 9 or 12 h respectively. After infection, cells were cultured for 1 week and as a result, the number of transduced SSCs increased significantly for MOI of 5 and 10 with 6 h of infection. When the same condition (MOI of 5 with 6 hours) was applied in presence or absence of STO feeder layer and infected SSCs were cultured for 3 weeks on the STO feeder layer, a significant increase in the number of transduced cells was observed for without the feeder layer during infection. We subsequently studied the effects of polycationic agents, polybrene and dioctadecylamidoglycyl spermine (DOGS), on the transduction efficiency. Compared with the polybrene treatment, the recovery rate of the transduced SSCs was significantly higher for the DOGS treatment. Therefore, our optimization study could contribute to the enhancement of germ-line modification of SSCs using lentiviral vectors and in generation of transgenic animals.
47 schema:genre research_article
48 schema:inLanguage en
49 schema:isAccessibleForFree true
50 schema:isPartOf N882ca80b47f14286aaa6efdeda249ba7
51 N9afd9eff5bab45f995b985706ec98324
52 sg:journal.1115754
53 schema:name Efficient enhancement of lentiviral transduction efficiency in murine spermatogonial stem cells
54 schema:pagination 449-455
55 schema:productId N1b661906ebb04f37a7fd20e9549bfc2d
56 N55cf45d3bb174cb8a7e76486e84e44f3
57 N6de8b6bcb74e441dafd6f10f028e103c
58 N715a150c40b64aa89942efc6b20f147a
59 Nb3847c6c541b4f9bb9f6b522e890d398
60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015319171
61 https://doi.org/10.1007/s10059-012-2167-7
62 schema:sdDatePublished 2019-04-10T13:07
63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
64 schema:sdPublisher N7c68ebea899b4d8a853c031057e61c63
65 schema:url http://link.springer.com/10.1007/s10059-012-2167-7
66 sgo:license sg:explorer/license/
67 sgo:sdDataset articles
68 rdf:type schema:ScholarlyArticle
69 N0359d78eedf442ca8f125092a6d5fba2 rdf:first sg:person.0617735407.02
70 rdf:rest N1c5d9e3e5d144f439be1066e37e10824
71 N146d45839fd042a7ba880b24cfcc5349 rdf:first sg:person.01315223635.05
72 rdf:rest N0359d78eedf442ca8f125092a6d5fba2
73 N1a7bee21965846549816743928d23d06 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Mice, Inbred C57BL
75 rdf:type schema:DefinedTerm
76 N1b661906ebb04f37a7fd20e9549bfc2d schema:name doi
77 schema:value 10.1007/s10059-012-2167-7
78 rdf:type schema:PropertyValue
79 N1bf98d25f7f44d389a2da3d558c10823 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Spermatogonia
81 rdf:type schema:DefinedTerm
82 N1c19bac40c014b08842c07f42fe04972 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Lentivirus
84 rdf:type schema:DefinedTerm
85 N1c5d9e3e5d144f439be1066e37e10824 rdf:first sg:person.0734164007.39
86 rdf:rest N5d3d8dbd86224edead93c9b0e8945e21
87 N41a76a8a93a349f3bb7673c0748a6453 rdf:first sg:person.01326265344.33
88 rdf:rest N146d45839fd042a7ba880b24cfcc5349
89 N4af03a81121947ed84d7a62f6d011fcf rdf:first sg:person.01171073502.48
90 rdf:rest rdf:nil
91 N4b0be83d34df4b4ba9d846697f664c05 rdf:first sg:person.0602661244.23
92 rdf:rest N41a76a8a93a349f3bb7673c0748a6453
93 N4d5543916546439cb6b45f00656996a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Animals, Genetically Modified
95 rdf:type schema:DefinedTerm
96 N55cf45d3bb174cb8a7e76486e84e44f3 schema:name dimensions_id
97 schema:value pub.1015319171
98 rdf:type schema:PropertyValue
99 N5817e21ee8ef4ca0b62a14e640cde5b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Genetic Vectors
101 rdf:type schema:DefinedTerm
102 N5912a2962bc94bbe8f0fe9d1fe4402a1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Male
104 rdf:type schema:DefinedTerm
105 N5ad0e6730dc44774be1e4bbb308a159d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Transduction, Genetic
107 rdf:type schema:DefinedTerm
108 N5d3d8dbd86224edead93c9b0e8945e21 rdf:first sg:person.01116635740.74
109 rdf:rest N4af03a81121947ed84d7a62f6d011fcf
110 N61f6ed9b8f5d4ee4ba5c8536d09a3f2d rdf:first sg:person.0706046135.57
111 rdf:rest Nc6fe414b5efb4f7ab3ddfa189d38dc98
112 N6de8b6bcb74e441dafd6f10f028e103c schema:name readcube_id
113 schema:value 48fd383855b3fcfc38fa4b1f8b522f34b0995c72d0cd7de2ddb938e49bacc3d7
114 rdf:type schema:PropertyValue
115 N715a150c40b64aa89942efc6b20f147a schema:name nlm_unique_id
116 schema:value 9610936
117 rdf:type schema:PropertyValue
118 N7c68ebea899b4d8a853c031057e61c63 schema:name Springer Nature - SN SciGraph project
119 rdf:type schema:Organization
120 N882ca80b47f14286aaa6efdeda249ba7 schema:volumeNumber 33
121 rdf:type schema:PublicationVolume
122 N8a976a336b2f44709ab134c42a4c8d42 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Animals
124 rdf:type schema:DefinedTerm
125 N8cc618af47c649a588d8f53866243300 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Stem Cells
127 rdf:type schema:DefinedTerm
128 N9afd9eff5bab45f995b985706ec98324 schema:issueNumber 5
129 rdf:type schema:PublicationIssue
130 N9c951f7b41ca4c6ba875191ed92c1d45 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Mice
132 rdf:type schema:DefinedTerm
133 Na5aab28c0f11417786c90217b83107fc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Spermatogenesis
135 rdf:type schema:DefinedTerm
136 Nb3847c6c541b4f9bb9f6b522e890d398 schema:name pubmed_id
137 schema:value 22526390
138 rdf:type schema:PropertyValue
139 Nb604214f94a6483e91764057d6a7bdde schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Cells, Cultured
141 rdf:type schema:DefinedTerm
142 Nc6fe414b5efb4f7ab3ddfa189d38dc98 rdf:first sg:person.0730322707.27
143 rdf:rest N4b0be83d34df4b4ba9d846697f664c05
144 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
145 schema:name Medical and Health Sciences
146 rdf:type schema:DefinedTerm
147 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
148 schema:name Medical Microbiology
149 rdf:type schema:DefinedTerm
150 sg:journal.1115754 schema:issn 0219-1032
151 1016-8478
152 schema:name Molecules and Cells
153 rdf:type schema:Periodical
154 sg:person.01116635740.74 schema:affiliation https://www.grid.ac/institutes/grid.49606.3d
155 schema:familyName Kim
156 schema:givenName Chul Geun
157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01116635740.74
158 rdf:type schema:Person
159 sg:person.01171073502.48 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
160 schema:familyName Ryu
161 schema:givenName Buom-Yong
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01171073502.48
163 rdf:type schema:Person
164 sg:person.01315223635.05 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
165 schema:familyName Kim
166 schema:givenName Byung-Gak
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315223635.05
168 rdf:type schema:Person
169 sg:person.01326265344.33 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
170 schema:familyName Lee
171 schema:givenName Yong-An
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01326265344.33
173 rdf:type schema:Person
174 sg:person.0602661244.23 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
175 schema:familyName Kim
176 schema:givenName Yong-Hee
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602661244.23
178 rdf:type schema:Person
179 sg:person.0617735407.02 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
180 schema:familyName Cho
181 schema:givenName Chul Min
182 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0617735407.02
183 rdf:type schema:Person
184 sg:person.0706046135.57 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
185 schema:familyName Kim
186 schema:givenName Bang-Jin
187 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0706046135.57
188 rdf:type schema:Person
189 sg:person.0730322707.27 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
190 schema:familyName Kim
191 schema:givenName Ki-Jung
192 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0730322707.27
193 rdf:type schema:Person
194 sg:person.0734164007.39 schema:affiliation https://www.grid.ac/institutes/grid.254224.7
195 schema:familyName Kang
196 schema:givenName Hye-Ryeon
197 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0734164007.39
198 rdf:type schema:Person
199 sg:pub.10.1007/978-3-642-68315-2_11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1081602573
200 https://doi.org/10.1007/978-3-642-68315-2_11
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/335088a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037763215
203 https://doi.org/10.1038/335088a0
204 rdf:type schema:CreativeWork
205 sg:pub.10.1038/nbt0396-339 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003259518
206 https://doi.org/10.1038/nbt0396-339
207 rdf:type schema:CreativeWork
208 sg:pub.10.1038/sj.gt.3300715 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029746234
209 https://doi.org/10.1038/sj.gt.3300715
210 rdf:type schema:CreativeWork
211 https://app.dimensions.ai/details/publication/pub.1079436472 schema:CreativeWork
212 https://app.dimensions.ai/details/publication/pub.1082936465 schema:CreativeWork
213 https://doi.org/10.1016/0076-6879(87)49054-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024313074
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/0092-8674(88)90020-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018961462
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/0304-4157(93)90004-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037861864
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/j.ydbio.2003.07.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033050974
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/j.ydbio.2004.07.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032636156
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/s0014-5793(02)03010-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032617529
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1073/pnas.0407063101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046300934
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1073/pnas.0506970102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008600171
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1073/pnas.0631767100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039001089
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1073/pnas.111158198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016710226
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1073/pnas.222561399 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049443866
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1073/pnas.84.21.7413 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022874269
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1073/pnas.91.24.11298 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047711366
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1073/pnas.91.24.11303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049656402
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1073/pnas.97.15.8346 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009438925
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1093/nar/24.15.3113 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042490645
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1095/biolreprod.103.016519 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041124466
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1095/biolreprod.103.017012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002372289
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1095/biolreprod.109.079558 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005725785
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1126/science.6272397 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062635980
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1182/blood-2001-11-0042 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019955154
254 rdf:type schema:CreativeWork
255 https://doi.org/10.2164/jandrol.106.001511 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015652573
256 rdf:type schema:CreativeWork
257 https://www.grid.ac/institutes/grid.254224.7 schema:alternateName Chung-Ang University
258 schema:name BET Research Institute, Chung-Ang University, 456-756, Ansung, Korea
259 Department of Animal Science and Technology, Chung-Ang University, 456-756, Ansung, Korea
260 rdf:type schema:Organization
261 https://www.grid.ac/institutes/grid.49606.3d schema:alternateName Hanyang University
262 schema:name Department of Life Science, College of Natural Sciences, Hanyang University, 133-791, Seoul, Korea
263 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...