A microarray-based method for detecting methylated loci View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2002-08

AUTHORS

Izuho Hatada, Azusa Kato, Sumiyo Morita, Yayoi Obata, Kayuri Nagaoka, Akira Sakurada, Masami Sato, Akira Horii, Atsumi Tsujimoto, Kenichi Matsubara

ABSTRACT

CpG island DNA methylation plays an important role in regulating gene expression in development and carcinogenesis. We developed a new microarray-based method called methylation amplification DNA chip (MAD) for detecting differences in methylation. In this method, only methylated CpG islands from the two samples that we wanted to compare were amplified and used for hybridization. The resource material for the microarray was derived from the methylated DNA library of the sample in which we wanted to detect hypermethylation. Choosing the methylated DNA library as the resource material of the microarray increased the percentage of DNA fragments derived from hypermethylated loci on the microarray. More... »

PAGES

448

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s100380200063

DOI

http://dx.doi.org/10.1007/s100380200063

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001772127

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/12181645


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167 Taisho Laboratory of Functional Genomics, Nara Institute of Science and Technology, Ikoma, Japan
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169 https://www.grid.ac/institutes/grid.452377.0 schema:alternateName DNA Chip Research (Japan)
170 schema:name DNA Chip Research Inc., Yokohama, Japan
171 rdf:type schema:Organization
172 https://www.grid.ac/institutes/grid.69566.3a schema:alternateName Tohoku University
173 schema:name Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Japan
174 Department of Thoracic Surgery, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan
175 rdf:type schema:Organization
 




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