Position-independent human β-globin gene expression mediated by a recombinant adeno-associated virus vector carrying the chicken β-globin insulator View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1999-05-01

AUTHORS

Haruyoshi Yamaza, Mizuki Ohno, Naotaka Hamasaki, Shin-ichi Mizuno, Takahito Inoue, Yasuyoshi Sakai, Yasuyuki Fukumaki

ABSTRACT

The position-independent expression of transgenes in target cells is an essential subject for determining effective gene therapies. The chicken beta-globin insulator blocks the effects of regulatory sequences on transcriptional units at differential domains. We prepared a recombinant adeno-associated virus (rAAV) containing various combinations of the DNase I-hypersensitive site 2 (HS2), 3 (HS3), and 4 (HS4) core elements from the human beta-globin locus control region (LCR), the human beta-globin gene, and the herpes virus thymidine kinase promoter driven neomycin-resistant gene (neoR) (rHS432, rHS43, rHS42, rHS32, and rHS2), and also rAAV containing two copies of the 250-bp core sequence of the chicken beta-globin insulator on both sides of the rHS2 (rIns/HS2/2Ins). After isolating neomycin-resistant mouse erythroleukemia (MEL) cells infected with each rAAV, we analyzed the rAAV genome by Southern blots and polymerase chain reaction (PCR), using primers specific for HS core elements and the human beta-globin gene. All clones contained a single copy of the rAAV genome in the chromosome, however, some of them had a rearranged proviral genome. In five clones with a single unrearranged rAAV genome for each rAAV construct, we assayed the expression of the human b-globin gene relative to the endogenous mouse beta maj-globin gene, using quantitative reverse transcriptase (RT)-PCR. In clones infected with rHS432, the expression level of the human beta-globin gene ranged from 51.6% to 765.6% of that in the mouse beta maj-globin gene. Likewise, in rHS43, the expression level ranged from 36.7% to 259.0%; in rHS42, from 47.8% to 207.0%; in rHS32, from 47.9% to 105.4%; and in rHS2, from 6.1% to 172.1%, indicating a high variability of expression level in clones infected with recombinant virus lacking the insulator. In contrast, in clones infected with rIns/HS2/Ins, the range of expression of the human beta-globin gene ranged from 52.8% to 58.3% of that in the mouse beta maj-globin gene. These results indicate that the insulator functioned dramatically to reduce the variability of transgene expression due to the position effect. This insulator-rAAV vector system holds promise to provide a constant level of transgene expression for gene therapy, regardless of the insertion sites on the chromosome. More... »

PAGES

152

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s100380050133

DOI

http://dx.doi.org/10.1007/s100380050133

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012435053

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10319578


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chickens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dependovirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Therapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Globins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HeLa Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leukemia, Erythroblastic, Acute", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Multigene Family", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Regulatory Sequences, Nucleic Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thalassemia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transformation, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Cells, Cultured", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Kyushu University", 
          "id": "https://www.grid.ac/institutes/grid.177174.3", 
          "name": [
            "Division of Disease Genes, Institute of Genetic Information, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yamaza", 
        "givenName": "Haruyoshi", 
        "id": "sg:person.01026710247.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026710247.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyushu University", 
          "id": "https://www.grid.ac/institutes/grid.177174.3", 
          "name": [
            "Division of Disease Genes, Institute of Genetic Information, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ohno", 
        "givenName": "Mizuki", 
        "id": "sg:person.013704070472.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013704070472.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyushu University", 
          "id": "https://www.grid.ac/institutes/grid.177174.3", 
          "name": [
            "Department of Clinical Chemistry and Laboratory Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hamasaki", 
        "givenName": "Naotaka", 
        "id": "sg:person.01261334035.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261334035.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyushu University", 
          "id": "https://www.grid.ac/institutes/grid.177174.3", 
          "name": [
            "Division of Disease Genes, Institute of Genetic Information, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mizuno", 
        "givenName": "Shin-ichi", 
        "id": "sg:person.011442753572.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011442753572.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyushu University", 
          "id": "https://www.grid.ac/institutes/grid.177174.3", 
          "name": [
            "Division of Disease Genes, Institute of Genetic Information, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Inoue", 
        "givenName": "Takahito", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyushu University", 
          "id": "https://www.grid.ac/institutes/grid.177174.3", 
          "name": [
            "Division of Disease Genes, Institute of Genetic Information, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sakai", 
        "givenName": "Yasuyoshi", 
        "id": "sg:person.01355266337.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355266337.88"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyushu University", 
          "id": "https://www.grid.ac/institutes/grid.177174.3", 
          "name": [
            "Division of Disease Genes, Institute of Genetic Information, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fukumaki", 
        "givenName": "Yasuyuki", 
        "id": "sg:person.010145100101.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010145100101.71"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1999-05-01", 
    "datePublishedReg": "1999-05-01", 
    "description": "The position-independent expression of transgenes in target cells is an essential subject for determining effective gene therapies. The chicken beta-globin insulator blocks the effects of regulatory sequences on transcriptional units at differential domains. We prepared a recombinant adeno-associated virus (rAAV) containing various combinations of the DNase I-hypersensitive site 2 (HS2), 3 (HS3), and 4 (HS4) core elements from the human beta-globin locus control region (LCR), the human beta-globin gene, and the herpes virus thymidine kinase promoter driven neomycin-resistant gene (neoR) (rHS432, rHS43, rHS42, rHS32, and rHS2), and also rAAV containing two copies of the 250-bp core sequence of the chicken beta-globin insulator on both sides of the rHS2 (rIns/HS2/2Ins). After isolating neomycin-resistant mouse erythroleukemia (MEL) cells infected with each rAAV, we analyzed the rAAV genome by Southern blots and polymerase chain reaction (PCR), using primers specific for HS core elements and the human beta-globin gene. All clones contained a single copy of the rAAV genome in the chromosome, however, some of them had a rearranged proviral genome. In five clones with a single unrearranged rAAV genome for each rAAV construct, we assayed the expression of the human b-globin gene relative to the endogenous mouse beta maj-globin gene, using quantitative reverse transcriptase (RT)-PCR. In clones infected with rHS432, the expression level of the human beta-globin gene ranged from 51.6% to 765.6% of that in the mouse beta maj-globin gene. Likewise, in rHS43, the expression level ranged from 36.7% to 259.0%; in rHS42, from 47.8% to 207.0%; in rHS32, from 47.9% to 105.4%; and in rHS2, from 6.1% to 172.1%, indicating a high variability of expression level in clones infected with recombinant virus lacking the insulator. In contrast, in clones infected with rIns/HS2/Ins, the range of expression of the human beta-globin gene ranged from 52.8% to 58.3% of that in the mouse beta maj-globin gene. These results indicate that the insulator functioned dramatically to reduce the variability of transgene expression due to the position effect. This insulator-rAAV vector system holds promise to provide a constant level of transgene expression for gene therapy, regardless of the insertion sites on the chromosome.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s100380050133", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1294984", 
        "issn": [
          "1434-5161", 
          "1435-232X"
        ], 
        "name": "Journal of Human Genetics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "44"
      }
    ], 
    "name": "Position-independent human \u03b2-globin gene expression mediated by a recombinant adeno-associated virus vector carrying the chicken \u03b2-globin insulator", 
    "pagination": "152", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3b3e49d85a62d0d3d0ec42dd770ee055b8cc70e4e9137675276782ce652b3721"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "10319578"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9808008"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s100380050133"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1012435053"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s100380050133", 
      "https://app.dimensions.ai/details/publication/pub.1012435053"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T22:19", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8690_00000422.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://www.nature.com/articles/jhg199938"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s100380050133'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s100380050133'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s100380050133'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s100380050133'


 

This table displays all metadata directly associated to this object as RDF triples.

175 TRIPLES      20 PREDICATES      44 URIs      36 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s100380050133 schema:about N1397697d8e3e41eba42639dc74db0fe6
2 N1a207c797bd2414e990f8b798f70bbf6
3 N28db1672f84849859039dedd96d71c94
4 N2b1691eb9073478dbfc68f663ade2604
5 N44ee61ca9c4348b096ce2bd5db69836e
6 N47de6b95d7d04ef7a62285fab2657ccf
7 N4de8134aee5946939af97ebe9ab58105
8 N6aaabaf66fd740e3bd80f35d8a7e9e6f
9 N724bd275956a48519ef959c1050ce3b2
10 N77bb38803ec442609eda929ce209aa3c
11 N8737f9bf536744b1a6aec6d309fdacaf
12 N8d996f9f086840e3a871177211aa4aab
13 N9dab7b2c1ecd46ae99c87e40a51153ea
14 Na0a7e5c6d1994189b01b74ad818c5c2c
15 Nb9171be2d1314e86a563804773f5d324
16 Nd18c75bc493d472eb6a41d4b8c8dcf5c
17 anzsrc-for:06
18 anzsrc-for:0604
19 schema:author Nd603ab11624f4e128a0680e31ec616a3
20 schema:datePublished 1999-05-01
21 schema:datePublishedReg 1999-05-01
22 schema:description The position-independent expression of transgenes in target cells is an essential subject for determining effective gene therapies. The chicken beta-globin insulator blocks the effects of regulatory sequences on transcriptional units at differential domains. We prepared a recombinant adeno-associated virus (rAAV) containing various combinations of the DNase I-hypersensitive site 2 (HS2), 3 (HS3), and 4 (HS4) core elements from the human beta-globin locus control region (LCR), the human beta-globin gene, and the herpes virus thymidine kinase promoter driven neomycin-resistant gene (neoR) (rHS432, rHS43, rHS42, rHS32, and rHS2), and also rAAV containing two copies of the 250-bp core sequence of the chicken beta-globin insulator on both sides of the rHS2 (rIns/HS2/2Ins). After isolating neomycin-resistant mouse erythroleukemia (MEL) cells infected with each rAAV, we analyzed the rAAV genome by Southern blots and polymerase chain reaction (PCR), using primers specific for HS core elements and the human beta-globin gene. All clones contained a single copy of the rAAV genome in the chromosome, however, some of them had a rearranged proviral genome. In five clones with a single unrearranged rAAV genome for each rAAV construct, we assayed the expression of the human b-globin gene relative to the endogenous mouse beta maj-globin gene, using quantitative reverse transcriptase (RT)-PCR. In clones infected with rHS432, the expression level of the human beta-globin gene ranged from 51.6% to 765.6% of that in the mouse beta maj-globin gene. Likewise, in rHS43, the expression level ranged from 36.7% to 259.0%; in rHS42, from 47.8% to 207.0%; in rHS32, from 47.9% to 105.4%; and in rHS2, from 6.1% to 172.1%, indicating a high variability of expression level in clones infected with recombinant virus lacking the insulator. In contrast, in clones infected with rIns/HS2/Ins, the range of expression of the human beta-globin gene ranged from 52.8% to 58.3% of that in the mouse beta maj-globin gene. These results indicate that the insulator functioned dramatically to reduce the variability of transgene expression due to the position effect. This insulator-rAAV vector system holds promise to provide a constant level of transgene expression for gene therapy, regardless of the insertion sites on the chromosome.
23 schema:genre research_article
24 schema:inLanguage en
25 schema:isAccessibleForFree true
26 schema:isPartOf Nb5e261b27093487088ecf9ce3aad42da
27 Ne1bfe113bac74681a874ee5efdd6bba0
28 sg:journal.1294984
29 schema:name Position-independent human β-globin gene expression mediated by a recombinant adeno-associated virus vector carrying the chicken β-globin insulator
30 schema:pagination 152
31 schema:productId N448927c0e0a74705988aa21f47f2be0a
32 N5236be88a5064f53840d2feda14f0c23
33 N60cdb71457b34991996e7659b80cd6ef
34 Nc6fc4124a23c49de8b6baeaeeda333db
35 Nd84c79a0339d490f97c1cbb1fdb0019e
36 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012435053
37 https://doi.org/10.1007/s100380050133
38 schema:sdDatePublished 2019-04-10T22:19
39 schema:sdLicense https://scigraph.springernature.com/explorer/license/
40 schema:sdPublisher N007f3325ce014671ab1c77babfd4bc2b
41 schema:url https://www.nature.com/articles/jhg199938
42 sgo:license sg:explorer/license/
43 sgo:sdDataset articles
44 rdf:type schema:ScholarlyArticle
45 N007f3325ce014671ab1c77babfd4bc2b schema:name Springer Nature - SN SciGraph project
46 rdf:type schema:Organization
47 N1397697d8e3e41eba42639dc74db0fe6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
48 schema:name Mice
49 rdf:type schema:DefinedTerm
50 N1a207c797bd2414e990f8b798f70bbf6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
51 schema:name Genetic Therapy
52 rdf:type schema:DefinedTerm
53 N255fa0f6446e4cf09e422c3a58645f68 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
54 schema:familyName Inoue
55 schema:givenName Takahito
56 rdf:type schema:Person
57 N28db1672f84849859039dedd96d71c94 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
58 schema:name Transformation, Genetic
59 rdf:type schema:DefinedTerm
60 N2b1691eb9073478dbfc68f663ade2604 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
61 schema:name Multigene Family
62 rdf:type schema:DefinedTerm
63 N3eb5d6ffe7fc47ff9aa913303aa1a28a rdf:first sg:person.011442753572.97
64 rdf:rest N3ee011e97dd14ee4ae235595cdf53f52
65 N3ee011e97dd14ee4ae235595cdf53f52 rdf:first N255fa0f6446e4cf09e422c3a58645f68
66 rdf:rest Nde930a3f0a084c4eb0a289aed80f5e87
67 N448927c0e0a74705988aa21f47f2be0a schema:name pubmed_id
68 schema:value 10319578
69 rdf:type schema:PropertyValue
70 N44ee61ca9c4348b096ce2bd5db69836e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Leukemia, Erythroblastic, Acute
72 rdf:type schema:DefinedTerm
73 N47de6b95d7d04ef7a62285fab2657ccf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Animals
75 rdf:type schema:DefinedTerm
76 N4de8134aee5946939af97ebe9ab58105 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name Regulatory Sequences, Nucleic Acid
78 rdf:type schema:DefinedTerm
79 N5236be88a5064f53840d2feda14f0c23 schema:name nlm_unique_id
80 schema:value 9808008
81 rdf:type schema:PropertyValue
82 N545606667b1c4262ab054f578150ff25 rdf:first sg:person.01261334035.16
83 rdf:rest N3eb5d6ffe7fc47ff9aa913303aa1a28a
84 N60cdb71457b34991996e7659b80cd6ef schema:name readcube_id
85 schema:value 3b3e49d85a62d0d3d0ec42dd770ee055b8cc70e4e9137675276782ce652b3721
86 rdf:type schema:PropertyValue
87 N6aaabaf66fd740e3bd80f35d8a7e9e6f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name HeLa Cells
89 rdf:type schema:DefinedTerm
90 N6ea8f8473add4a798097cc3bc53075f8 rdf:first sg:person.010145100101.71
91 rdf:rest rdf:nil
92 N724bd275956a48519ef959c1050ce3b2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Gene Expression
94 rdf:type schema:DefinedTerm
95 N77bb38803ec442609eda929ce209aa3c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Tumor Cells, Cultured
97 rdf:type schema:DefinedTerm
98 N8737f9bf536744b1a6aec6d309fdacaf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Chickens
100 rdf:type schema:DefinedTerm
101 N8d996f9f086840e3a871177211aa4aab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Globins
103 rdf:type schema:DefinedTerm
104 N99bb8e230b3043888a32cc3aa5be2242 rdf:first sg:person.013704070472.19
105 rdf:rest N545606667b1c4262ab054f578150ff25
106 N9dab7b2c1ecd46ae99c87e40a51153ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Thalassemia
108 rdf:type schema:DefinedTerm
109 Na0a7e5c6d1994189b01b74ad818c5c2c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
110 schema:name Dependovirus
111 rdf:type schema:DefinedTerm
112 Nb5e261b27093487088ecf9ce3aad42da schema:issueNumber 3
113 rdf:type schema:PublicationIssue
114 Nb9171be2d1314e86a563804773f5d324 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Genetic Vectors
116 rdf:type schema:DefinedTerm
117 Nc6fc4124a23c49de8b6baeaeeda333db schema:name dimensions_id
118 schema:value pub.1012435053
119 rdf:type schema:PropertyValue
120 Nd18c75bc493d472eb6a41d4b8c8dcf5c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Humans
122 rdf:type schema:DefinedTerm
123 Nd603ab11624f4e128a0680e31ec616a3 rdf:first sg:person.01026710247.45
124 rdf:rest N99bb8e230b3043888a32cc3aa5be2242
125 Nd84c79a0339d490f97c1cbb1fdb0019e schema:name doi
126 schema:value 10.1007/s100380050133
127 rdf:type schema:PropertyValue
128 Nde930a3f0a084c4eb0a289aed80f5e87 rdf:first sg:person.01355266337.88
129 rdf:rest N6ea8f8473add4a798097cc3bc53075f8
130 Ne1bfe113bac74681a874ee5efdd6bba0 schema:volumeNumber 44
131 rdf:type schema:PublicationVolume
132 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
133 schema:name Biological Sciences
134 rdf:type schema:DefinedTerm
135 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
136 schema:name Genetics
137 rdf:type schema:DefinedTerm
138 sg:journal.1294984 schema:issn 1434-5161
139 1435-232X
140 schema:name Journal of Human Genetics
141 rdf:type schema:Periodical
142 sg:person.010145100101.71 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
143 schema:familyName Fukumaki
144 schema:givenName Yasuyuki
145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010145100101.71
146 rdf:type schema:Person
147 sg:person.01026710247.45 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
148 schema:familyName Yamaza
149 schema:givenName Haruyoshi
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026710247.45
151 rdf:type schema:Person
152 sg:person.011442753572.97 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
153 schema:familyName Mizuno
154 schema:givenName Shin-ichi
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011442753572.97
156 rdf:type schema:Person
157 sg:person.01261334035.16 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
158 schema:familyName Hamasaki
159 schema:givenName Naotaka
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01261334035.16
161 rdf:type schema:Person
162 sg:person.01355266337.88 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
163 schema:familyName Sakai
164 schema:givenName Yasuyoshi
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355266337.88
166 rdf:type schema:Person
167 sg:person.013704070472.19 schema:affiliation https://www.grid.ac/institutes/grid.177174.3
168 schema:familyName Ohno
169 schema:givenName Mizuki
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013704070472.19
171 rdf:type schema:Person
172 https://www.grid.ac/institutes/grid.177174.3 schema:alternateName Kyushu University
173 schema:name Department of Clinical Chemistry and Laboratory Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
174 Division of Disease Genes, Institute of Genetic Information, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan
175 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...