Trace determination of vardenafil hydrochloride in commercial formulation and human serum by adsorptive anodic stripping voltammetry at a carbon paste ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-07

AUTHORS

Mohamed M. Ghoneim, Amera M. Hassanein, Nehal A. Salahuddin, Hanaa S. El-Desoky, Mona N. Elfiky

ABSTRACT

The electrochemical behavior of vardenafil HCl (VRL) at a carbon paste electrode (CPE) was investigated by cyclic voltammetry, and the mechanism of its oxidation was suggested and discussed. A simple Nujol-based CPE in combination with a sensitive square-wave adsorption anodic stripping voltammetry method was described for trace determination of VRL. The described method showed excellent performance for trace determination of VRL in its formulation “Levitra® tablets” without interference from excipients. The results were statistically compared with those obtained with an established HPLC method; nonsignificant differences were found between the described voltammetric and HPLC methods. The described stripping voltammetric method is highly sensitive (limit of detection = 3 × 10−10 mol L−1 and limit of quantitation = 1 × 10−9 mol L−1). It was successfully applied for the determination of VRL in spiked human serum without the necessity for pretreatment and/or time-consuming extraction steps prior to the analysis. More... »

PAGES

1891-1902

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s10008-013-2020-8

DOI

http://dx.doi.org/10.1007/s10008-013-2020-8

DIMENSIONS

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