Photodamaging Effects of Porphyrin in a Human Carcinoma Cell Line View Full Text


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Article Info

DATE

2000-02

AUTHORS

M. Gabriela Alvarez, Mariángeles La Penna, E. Ines Yslas, Viviana Rivarola, Edgardo N. Durantini

ABSTRACT

A convenient procedure to visualize the photodynamic effect of porphyrins on cell lines is shown. 5,10,15,20-Tetra(4-methoxyphenyl)porphyrin (TMP) was used as photosensitizer. The culture flasks bearing a Hep-2 cell line were incubated for 24 h with 1 μM of TMP. Under these conditions saturation of the TMP intracellular concentration is obtained. The irradiation of cell cultures for 30 min produces 90% cell mortality, while no toxicity was observed under dark conditions or under irradiation without TMP. This methodology can be used to demonstrate the photodynamic therapy (PDT) process in a laboratory experiment. More... »

PAGES

24-26

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00897990354a

DOI

http://dx.doi.org/10.1007/s00897990354a

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044662173


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