Validation and application of a novel APC antibody in western blotting, immunoprecipitation, and immunohistochemistry View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-12

AUTHORS

Nami O. Yamada, Wenduerma, Shuji Matsuda, Takao Senda

ABSTRACT

Adenomatous polyposis coli (APC) is a large protein with multiple binding partners, suggesting diverse functions besides its well-known role in the destruction of β-catenin. To elucidate these complex functions, it is crucial to evaluate the precise subcellular distribution of APC within a cell and tissue. However, most of the commercially available anti-APC antibodies can only be used for limited applications, resulting in the use of independently generated antibodies. This has led to various discrepancies between studies as a common antibody has not been established. In this study, we generated an antibody against the c-terminal domain of human APC, designated APC-C antibody, and evaluated its specificity and application in various immunological methods. Our data indicate that this novel APC-C antibody is a specific and versatile antibody that can be used in western blotting, immunoprecipitation, immunocytochemistry, and immunohistochemistry. Widespread use of this APC antibody will help enhance our understanding of APC's function in both normal and cancer cell biology. More... »

PAGES

227-236

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00795-018-0196-9

DOI

http://dx.doi.org/10.1007/s00795-018-0196-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1104991473

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29923125


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