Mice lacking functional CD95-ligand display reduced proliferation of the intestinal epithelium without gross homeostatic alterations View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-06

AUTHORS

Kari Trumpi, Ernst J. A. Steller, Wendy W. de Leng, Daniëlle A. Raats, Isaäc J. Nijman, Folkert H. M. Morsink, Inne H. M. Borel Rinkes, Onno Kranenburg

ABSTRACT

Homeostasis of the continuously self-renewing intestinal tract involves cell proliferation, migration, differentiation along the crypt-villus-axis and shedding of cells into the gut lumen. CD95-ligand (FAS-ligand, CD95L) is a cytokine that is known for its capacity to induce apoptosis by binding its cognate receptor, CD95 (Fas). More recently, it was discovered that CD95L can also induce other cellular responses, such as proliferation, differentiation and cell migration. CD95L is highly expressed in Paneth cells of the small intestine which are in close contact with intestinal stem cells. This suggests a potential role for CD95L in controlling stem cell function and, possibly, intestinal homeostasis. We analyzed the intestines of mice deficient for functional CD95L (gld) for potential alterations in the diversity of stem-cell-lineages and parameters of intestinal homeostasis. Stem cell diversity was assessed by analyzing methylation patterns of the non-transcribed mMYOD gene. Proliferation was analyzed by BrdU labeling and differentiation was assessed by immunohistochemistry. Of all parameters analyzed, only epithelial cell proliferation was significantly reduced in the small intestines of gld-mice, but not in their colons which lack CD95L expression. We conclude that CD95L has a proliferation-stimulating role during normal turnover of the small intestine, but has a marginal effect on overall intestinal homeostasis. More... »

PAGES

110-118

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00795-015-0129-9

DOI

http://dx.doi.org/10.1007/s00795-015-0129-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007295376

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/26700225


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Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00795-015-0129-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00795-015-0129-9'


 

This table displays all metadata directly associated to this object as RDF triples.

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