Establishment of gene transfer and gene silencing methods in a desiccation-tolerant cell line, Pv11 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-01

AUTHORS

Yoichiro Sogame, Jun Okada, Shingo Kikuta, Yugo Miyata, Richard Cornette, Oleg Gusev, Takahiro Kikawada

ABSTRACT

Larvae of the African midge Polypedilum vanderplanki show extreme desiccation tolerance, known as anhydrobiosis. Recently, the cultured cell line Pv11 was derived from this species; Pv11 cells can be preserved in the dry state for over 6 months and retain their proliferation potential. Here, we attempted to expand the use of Pv11 cells as a model to investigate the mechanisms underlying anhydrobiosis in P. vanderplanki. A newly developed vector comprising a constitutive promoter for the PvGapdh gene allowed the expression of exogenous proteins in Pv11 cells. Using this vector, a stable Pv11 cell line expressing green fluorescence protein (GFP) was established and retained desiccation tolerance. Gene silencing with GFP-specific siRNAs significantly suppressed GFP expression to approximately 7.5-34.6% of that in the non-siRNA-transfected GFP stable line. Establishment of these functional assays will enable Pv11 cells to be utilized as an effective tool to investigate the molecular mechanisms underlying anhydrobiosis. More... »

PAGES

65-72

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00792-016-0880-4

DOI

http://dx.doi.org/10.1007/s00792-016-0880-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1051085758

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27757696


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