Carboxylation reaction catalyzed by 2-oxoglutarate:ferredoxin oxidoreductases from Hydrogenobacter thermophilus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-01

AUTHORS

Masahiro Yamamoto, Takeshi Ikeda, Hiroyuki Arai, Masaharu Ishii, Yasuo Igarashi

ABSTRACT

Hydrogenobacter thermophilus TK-6 is a thermophilic, chemolithoautotrophic, hydrogen-oxidizing bacterium that fixes carbon dioxide via the reductive tricarboxylic acid (rTCA) cycle. 2-Oxoglutarate:ferredoxin oxidoreductase (OGOR) is the key enzyme in this cycle that fixes carbon dioxide. The genome of strain TK-6 encodes at least two distinct OGOR enzymes, termed For and Kor. We report here a method for measuring the carboxylation of succinyl-CoA catalyzed by OGORs. The method involves the in vitro coupling of OGOR with ferredoxin and pyruvate:ferredoxin oxidoreductase from strain TK-6, and glutamate dehydrogenase from Sulfolobus tokodaii. Using this method, we determined both the apparent maximum velocities and the K (m) values of For and Kor for the carboxylation of succinyl-CoA. This is the first reported kinetic analysis of carbon fixation catalyzed by OGOR enzymes from the rTCA cycle. More... »

PAGES

79-85

Journal

TITLE

Extremophiles

ISSUE

1

VOLUME

14

Author Affiliations

From Grant

  • Related Patents

  • Microorganisms And Methods For The Biosynthesis Of Adipate, Hexamethylenediamine And 6-Aminocaproic Acid
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Methods And Systems For Chemoautotrophic Production Of Organic Compounds
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms And Methods For The Biosynthesis Of Butadiene
  • Process Of Separating Components Of A Fermentation Broth
  • Semi-Synthetic Terephthalic Acid Via Microorganisms That Produce Muconic Acid
  • Microorganisms And Methods For Enhancing The Availability Of Reducing Equivalents In The Presence Of Methanol, For Producing Methacrylic Acid
  • Microorganisms And Methods For The Biosynthesis Of Propylene
  • Microorganisms And Methods For The Production Of Caprolactone
  • Microorganisms And Methods For Carbon-Efficient Biosynthesis Of Mek And 2-Butanol
  • Microorganisms For The Production Of 1,4-Butanediol And Related Methods
  • Microorganisms And Methods For The Coproduction 1,4-Butanediol And Gamma-Butyrolactone
  • Microorganisms For Producing Propylene And Methods Related Thereto
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms And Methods For The Coproduction 1,4-Butanediol And Gamma-Butyrolactone
  • Microorganisms And Methods For The Biosynthesis Of Fumarate, Malate, And Acrylate
  • Microorganisms For The Production Of 2-Hydroxyisobutyric Acid
  • Microorganisms For The Production Of Methacrylic Acid
  • Microorganisms And Methods For The Biosynthesis Of Aromatics, 2,4-Pentadienoate And 1,3-Butadiene
  • Microorganisms For Producing Butadiene And Methods Related Thereto
  • Microorganisms And Methods For The Production Of Caprolactone
  • Organisms For The Production Of 1,3-Butanediol
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00792-009-0289-4

    DOI

    http://dx.doi.org/10.1007/s00792-009-0289-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1037168372

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19894084


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Acyl Coenzyme A", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacteria", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacterial Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Citric Acid Cycle", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genome, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Ketone Oxidoreductases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Kinetics", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Tokyo", 
              "id": "https://www.grid.ac/institutes/grid.26999.3d", 
              "name": [
                "Department of Biotechnology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113-8657, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yamamoto", 
            "givenName": "Masahiro", 
            "id": "sg:person.01275142271.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01275142271.43"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Tokyo", 
              "id": "https://www.grid.ac/institutes/grid.26999.3d", 
              "name": [
                "Department of Biotechnology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113-8657, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ikeda", 
            "givenName": "Takeshi", 
            "id": "sg:person.0663470155.57", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663470155.57"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Tokyo", 
              "id": "https://www.grid.ac/institutes/grid.26999.3d", 
              "name": [
                "Department of Biotechnology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113-8657, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Arai", 
            "givenName": "Hiroyuki", 
            "id": "sg:person.01042115652.40", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042115652.40"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Tokyo", 
              "id": "https://www.grid.ac/institutes/grid.26999.3d", 
              "name": [
                "Department of Biotechnology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113-8657, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Ishii", 
            "givenName": "Masaharu", 
            "id": "sg:person.0733071477.72", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733071477.72"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Tokyo", 
              "id": "https://www.grid.ac/institutes/grid.26999.3d", 
              "name": [
                "Department of Biotechnology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113-8657, Tokyo, Japan"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Igarashi", 
            "givenName": "Yasuo", 
            "id": "sg:person.01165444433.47", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01165444433.47"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1111/j.1574-6968.1996.tb08193.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006728346"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.1996.tb08193.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006728346"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1462-2920.2006.01118.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010886796"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1263/jbb.104.470", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010914281"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.55.4.928", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015108543"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.184.14.3975-3983.2002", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016098956"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.187.9.3020-3027.2005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016852526"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00292082", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017191741", 
              "https://doi.org/10.1007/bf00292082"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2958.2006.05399.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017772170"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2958.2006.05399.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017772170"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.00747-08", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018517372"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1263/jbb.101.391", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019576625"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/bbrc.2002.6651", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024333796"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.01360-06", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025463121"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1271/bbb.61.510", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029651134"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002030050443", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033183103", 
              "https://doi.org/10.1007/s002030050443"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2958.2004.04010.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033418542"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2958.2004.04009.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035485862"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0065-3233(08)60362-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036179925"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.2006.00415.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036546408"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1006/bbrc.2001.4542", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038666830"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1074/jbc.m003291200", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040323163"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0723-2020(11)80207-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041857069"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/dnares/8.4.123", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042323023"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1263/jbb.102.311", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1042640851"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1271/bbb.69.1172", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043747750"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1271/bbb1961.44.1985", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044942095"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbrc.2003.11.078", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048827420"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00422307", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050322817", 
              "https://doi.org/10.1007/bf00422307"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1271/bbb.64.492", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050469048"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.00138-06", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051721096"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.bbrc.2005.11.155", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052726902"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/aem.71.11.7310-7320.2005", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052879005"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/00207713-34-1-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060347764"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/00207713-44-4-620", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060349010"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.178.11.3365-3368.1996", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062724891"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080486938", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2010-01", 
        "datePublishedReg": "2010-01-01", 
        "description": "Hydrogenobacter thermophilus TK-6 is a thermophilic, chemolithoautotrophic, hydrogen-oxidizing bacterium that fixes carbon dioxide via the reductive tricarboxylic acid (rTCA) cycle. 2-Oxoglutarate:ferredoxin oxidoreductase (OGOR) is the key enzyme in this cycle that fixes carbon dioxide. The genome of strain TK-6 encodes at least two distinct OGOR enzymes, termed For and Kor. We report here a method for measuring the carboxylation of succinyl-CoA catalyzed by OGORs. The method involves the in vitro coupling of OGOR with ferredoxin and pyruvate:ferredoxin oxidoreductase from strain TK-6, and glutamate dehydrogenase from Sulfolobus tokodaii. Using this method, we determined both the apparent maximum velocities and the K (m) values of For and Kor for the carboxylation of succinyl-CoA. This is the first reported kinetic analysis of carbon fixation catalyzed by OGOR enzymes from the rTCA cycle.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s00792-009-0289-4", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.6008722", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1116821", 
            "issn": [
              "1431-0651", 
              "1433-4909"
            ], 
            "name": "Extremophiles", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "14"
          }
        ], 
        "name": "Carboxylation reaction catalyzed by 2-oxoglutarate:ferredoxin oxidoreductases from Hydrogenobacter thermophilus", 
        "pagination": "79-85", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "b10e8ea047fe5de7c47320b29e737abed40481c1eec39d2f00c1cccf750bb1c7"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "19894084"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "9706854"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00792-009-0289-4"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1037168372"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00792-009-0289-4", 
          "https://app.dimensions.ai/details/publication/pub.1037168372"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T14:26", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000373_0000000373/records_13071_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs00792-009-0289-4"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00792-009-0289-4'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00792-009-0289-4'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00792-009-0289-4'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00792-009-0289-4'


     

    This table displays all metadata directly associated to this object as RDF triples.

    234 TRIPLES      21 PREDICATES      71 URIs      28 LITERALS      16 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00792-009-0289-4 schema:about N03862bfdf7b34fc5af947f5cfe124caa
    2 N17b37be43c5c4328b369d02543202c82
    3 N45ee64a8eed64630a0b8eb4d3a3ced6a
    4 N581733a8f82d45c9839ffb0ee3b7f017
    5 N8785286fc1414da481fa2d1aeadc29db
    6 Ne3bdef8be09b4cbd9dc33b5d7b53c2ae
    7 Ne9ba32f5b59a487c9f9348f962f5d563
    8 anzsrc-for:06
    9 anzsrc-for:0601
    10 schema:author N36c920b172ad41e9bb015f46a5bc4b8a
    11 schema:citation sg:pub.10.1007/bf00292082
    12 sg:pub.10.1007/bf00422307
    13 sg:pub.10.1007/s002030050443
    14 https://app.dimensions.ai/details/publication/pub.1080486938
    15 https://doi.org/10.1006/bbrc.2001.4542
    16 https://doi.org/10.1006/bbrc.2002.6651
    17 https://doi.org/10.1016/j.bbrc.2003.11.078
    18 https://doi.org/10.1016/j.bbrc.2005.11.155
    19 https://doi.org/10.1016/s0065-3233(08)60362-9
    20 https://doi.org/10.1016/s0723-2020(11)80207-9
    21 https://doi.org/10.1073/pnas.55.4.928
    22 https://doi.org/10.1074/jbc.m003291200
    23 https://doi.org/10.1093/dnares/8.4.123
    24 https://doi.org/10.1099/00207713-34-1-5
    25 https://doi.org/10.1099/00207713-44-4-620
    26 https://doi.org/10.1111/j.1365-2958.2004.04009.x
    27 https://doi.org/10.1111/j.1365-2958.2004.04010.x
    28 https://doi.org/10.1111/j.1365-2958.2006.05399.x
    29 https://doi.org/10.1111/j.1462-2920.2006.01118.x
    30 https://doi.org/10.1111/j.1574-6968.1996.tb08193.x
    31 https://doi.org/10.1111/j.1574-6968.2006.00415.x
    32 https://doi.org/10.1128/aem.71.11.7310-7320.2005
    33 https://doi.org/10.1128/jb.00138-06
    34 https://doi.org/10.1128/jb.00747-08
    35 https://doi.org/10.1128/jb.01360-06
    36 https://doi.org/10.1128/jb.178.11.3365-3368.1996
    37 https://doi.org/10.1128/jb.184.14.3975-3983.2002
    38 https://doi.org/10.1128/jb.187.9.3020-3027.2005
    39 https://doi.org/10.1263/jbb.101.391
    40 https://doi.org/10.1263/jbb.102.311
    41 https://doi.org/10.1263/jbb.104.470
    42 https://doi.org/10.1271/bbb.61.510
    43 https://doi.org/10.1271/bbb.64.492
    44 https://doi.org/10.1271/bbb.69.1172
    45 https://doi.org/10.1271/bbb1961.44.1985
    46 schema:datePublished 2010-01
    47 schema:datePublishedReg 2010-01-01
    48 schema:description Hydrogenobacter thermophilus TK-6 is a thermophilic, chemolithoautotrophic, hydrogen-oxidizing bacterium that fixes carbon dioxide via the reductive tricarboxylic acid (rTCA) cycle. 2-Oxoglutarate:ferredoxin oxidoreductase (OGOR) is the key enzyme in this cycle that fixes carbon dioxide. The genome of strain TK-6 encodes at least two distinct OGOR enzymes, termed For and Kor. We report here a method for measuring the carboxylation of succinyl-CoA catalyzed by OGORs. The method involves the in vitro coupling of OGOR with ferredoxin and pyruvate:ferredoxin oxidoreductase from strain TK-6, and glutamate dehydrogenase from Sulfolobus tokodaii. Using this method, we determined both the apparent maximum velocities and the K (m) values of For and Kor for the carboxylation of succinyl-CoA. This is the first reported kinetic analysis of carbon fixation catalyzed by OGOR enzymes from the rTCA cycle.
    49 schema:genre research_article
    50 schema:inLanguage en
    51 schema:isAccessibleForFree false
    52 schema:isPartOf N3757053ace63464190e7ac96d3aa70c7
    53 Nb01b25456ae34a7b89ccb03613e083ef
    54 sg:journal.1116821
    55 schema:name Carboxylation reaction catalyzed by 2-oxoglutarate:ferredoxin oxidoreductases from Hydrogenobacter thermophilus
    56 schema:pagination 79-85
    57 schema:productId N0ed1360147b445298c6821ba005b4edb
    58 N473ab6bef5994e0bbec8870d024182e4
    59 N741a8b5f003b4c5a80f8b73b278d3073
    60 N7cae02dc7015410baa189b0b983b8106
    61 Nc9958eef8540450bba6f341e0cdcac58
    62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037168372
    63 https://doi.org/10.1007/s00792-009-0289-4
    64 schema:sdDatePublished 2019-04-11T14:26
    65 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    66 schema:sdPublisher N21282f08d6614bb598219f1642e4aa5a
    67 schema:url http://link.springer.com/10.1007%2Fs00792-009-0289-4
    68 sgo:license sg:explorer/license/
    69 sgo:sdDataset articles
    70 rdf:type schema:ScholarlyArticle
    71 N03862bfdf7b34fc5af947f5cfe124caa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    72 schema:name Bacteria
    73 rdf:type schema:DefinedTerm
    74 N0c9f90f5b49448bfb6dd7f4ccc4f725d rdf:first sg:person.0663470155.57
    75 rdf:rest N90bd4f1707524dcc879734f7d6daae4b
    76 N0ed1360147b445298c6821ba005b4edb schema:name nlm_unique_id
    77 schema:value 9706854
    78 rdf:type schema:PropertyValue
    79 N13c853cb60c44bc8bda670e6cd6872cc rdf:first sg:person.01165444433.47
    80 rdf:rest rdf:nil
    81 N17b37be43c5c4328b369d02543202c82 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    82 schema:name Citric Acid Cycle
    83 rdf:type schema:DefinedTerm
    84 N21282f08d6614bb598219f1642e4aa5a schema:name Springer Nature - SN SciGraph project
    85 rdf:type schema:Organization
    86 N36c920b172ad41e9bb015f46a5bc4b8a rdf:first sg:person.01275142271.43
    87 rdf:rest N0c9f90f5b49448bfb6dd7f4ccc4f725d
    88 N3757053ace63464190e7ac96d3aa70c7 schema:issueNumber 1
    89 rdf:type schema:PublicationIssue
    90 N45ee64a8eed64630a0b8eb4d3a3ced6a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    91 schema:name Acyl Coenzyme A
    92 rdf:type schema:DefinedTerm
    93 N473ab6bef5994e0bbec8870d024182e4 schema:name readcube_id
    94 schema:value b10e8ea047fe5de7c47320b29e737abed40481c1eec39d2f00c1cccf750bb1c7
    95 rdf:type schema:PropertyValue
    96 N581733a8f82d45c9839ffb0ee3b7f017 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    97 schema:name Genome, Bacterial
    98 rdf:type schema:DefinedTerm
    99 N741a8b5f003b4c5a80f8b73b278d3073 schema:name dimensions_id
    100 schema:value pub.1037168372
    101 rdf:type schema:PropertyValue
    102 N7cae02dc7015410baa189b0b983b8106 schema:name doi
    103 schema:value 10.1007/s00792-009-0289-4
    104 rdf:type schema:PropertyValue
    105 N8785286fc1414da481fa2d1aeadc29db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    106 schema:name Ketone Oxidoreductases
    107 rdf:type schema:DefinedTerm
    108 N90bd4f1707524dcc879734f7d6daae4b rdf:first sg:person.01042115652.40
    109 rdf:rest N9b0768ded48246e993e85c12be2dbb83
    110 N9b0768ded48246e993e85c12be2dbb83 rdf:first sg:person.0733071477.72
    111 rdf:rest N13c853cb60c44bc8bda670e6cd6872cc
    112 Nb01b25456ae34a7b89ccb03613e083ef schema:volumeNumber 14
    113 rdf:type schema:PublicationVolume
    114 Nc9958eef8540450bba6f341e0cdcac58 schema:name pubmed_id
    115 schema:value 19894084
    116 rdf:type schema:PropertyValue
    117 Ne3bdef8be09b4cbd9dc33b5d7b53c2ae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name Kinetics
    119 rdf:type schema:DefinedTerm
    120 Ne9ba32f5b59a487c9f9348f962f5d563 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    121 schema:name Bacterial Proteins
    122 rdf:type schema:DefinedTerm
    123 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    124 schema:name Biological Sciences
    125 rdf:type schema:DefinedTerm
    126 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    127 schema:name Biochemistry and Cell Biology
    128 rdf:type schema:DefinedTerm
    129 sg:grant.6008722 http://pending.schema.org/fundedItem sg:pub.10.1007/s00792-009-0289-4
    130 rdf:type schema:MonetaryGrant
    131 sg:journal.1116821 schema:issn 1431-0651
    132 1433-4909
    133 schema:name Extremophiles
    134 rdf:type schema:Periodical
    135 sg:person.01042115652.40 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
    136 schema:familyName Arai
    137 schema:givenName Hiroyuki
    138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01042115652.40
    139 rdf:type schema:Person
    140 sg:person.01165444433.47 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
    141 schema:familyName Igarashi
    142 schema:givenName Yasuo
    143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01165444433.47
    144 rdf:type schema:Person
    145 sg:person.01275142271.43 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
    146 schema:familyName Yamamoto
    147 schema:givenName Masahiro
    148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01275142271.43
    149 rdf:type schema:Person
    150 sg:person.0663470155.57 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
    151 schema:familyName Ikeda
    152 schema:givenName Takeshi
    153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663470155.57
    154 rdf:type schema:Person
    155 sg:person.0733071477.72 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
    156 schema:familyName Ishii
    157 schema:givenName Masaharu
    158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0733071477.72
    159 rdf:type schema:Person
    160 sg:pub.10.1007/bf00292082 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017191741
    161 https://doi.org/10.1007/bf00292082
    162 rdf:type schema:CreativeWork
    163 sg:pub.10.1007/bf00422307 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050322817
    164 https://doi.org/10.1007/bf00422307
    165 rdf:type schema:CreativeWork
    166 sg:pub.10.1007/s002030050443 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033183103
    167 https://doi.org/10.1007/s002030050443
    168 rdf:type schema:CreativeWork
    169 https://app.dimensions.ai/details/publication/pub.1080486938 schema:CreativeWork
    170 https://doi.org/10.1006/bbrc.2001.4542 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038666830
    171 rdf:type schema:CreativeWork
    172 https://doi.org/10.1006/bbrc.2002.6651 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024333796
    173 rdf:type schema:CreativeWork
    174 https://doi.org/10.1016/j.bbrc.2003.11.078 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048827420
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1016/j.bbrc.2005.11.155 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052726902
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1016/s0065-3233(08)60362-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036179925
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1016/s0723-2020(11)80207-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041857069
    181 rdf:type schema:CreativeWork
    182 https://doi.org/10.1073/pnas.55.4.928 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015108543
    183 rdf:type schema:CreativeWork
    184 https://doi.org/10.1074/jbc.m003291200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040323163
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1093/dnares/8.4.123 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042323023
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1099/00207713-34-1-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060347764
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.1099/00207713-44-4-620 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060349010
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.1111/j.1365-2958.2004.04009.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1035485862
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.1111/j.1365-2958.2004.04010.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1033418542
    195 rdf:type schema:CreativeWork
    196 https://doi.org/10.1111/j.1365-2958.2006.05399.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1017772170
    197 rdf:type schema:CreativeWork
    198 https://doi.org/10.1111/j.1462-2920.2006.01118.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010886796
    199 rdf:type schema:CreativeWork
    200 https://doi.org/10.1111/j.1574-6968.1996.tb08193.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006728346
    201 rdf:type schema:CreativeWork
    202 https://doi.org/10.1111/j.1574-6968.2006.00415.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1036546408
    203 rdf:type schema:CreativeWork
    204 https://doi.org/10.1128/aem.71.11.7310-7320.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052879005
    205 rdf:type schema:CreativeWork
    206 https://doi.org/10.1128/jb.00138-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051721096
    207 rdf:type schema:CreativeWork
    208 https://doi.org/10.1128/jb.00747-08 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018517372
    209 rdf:type schema:CreativeWork
    210 https://doi.org/10.1128/jb.01360-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025463121
    211 rdf:type schema:CreativeWork
    212 https://doi.org/10.1128/jb.178.11.3365-3368.1996 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062724891
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1128/jb.184.14.3975-3983.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016098956
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1128/jb.187.9.3020-3027.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016852526
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1263/jbb.101.391 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019576625
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1263/jbb.102.311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042640851
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1263/jbb.104.470 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010914281
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1271/bbb.61.510 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029651134
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1271/bbb.64.492 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050469048
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1271/bbb.69.1172 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043747750
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1271/bbb1961.44.1985 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044942095
    231 rdf:type schema:CreativeWork
    232 https://www.grid.ac/institutes/grid.26999.3d schema:alternateName University of Tokyo
    233 schema:name Department of Biotechnology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113-8657, Tokyo, Japan
    234 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...