Characterization of malate dehydrogenase from the hyperthermophilic archaeon Pyrobaculum islandicum View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-09

AUTHORS

Lynda J. Yennaco, Yajing Hu, James F. Holden

ABSTRACT

Native and recombinant malate dehydrogenase (MDH) was characterized from the hyperthermophilic, facultatively autotrophic archaeon Pyrobaculum islandicum. The enzyme is a homotetramer with a subunit mass of 33 kDa. The activity kinetics of the native and recombinant proteins are the same. The apparent K ( m ) values of the recombinant protein for oxaloacetate (OAA) and NADH (at 80 degrees C and pH 8.0) were 15 and 86 microM, respectively, with specific activity as high as 470 U mg(-1). Activity decreased more than 90% when NADPH was used. The catalytic efficiency of OAA reduction by P. islandicum MDH using NADH was significantly higher than that reported for any other archaeal MDH. Unlike other archaeal MDHs, specific activity of the P. islandicum MDH back-reaction also decreased more than 90% when malate and NAD(+) were used as substrates and was not detected with NADP(+). A phylogenetic tree of 31 archaeal MDHs shows that they fall into 5 distinct groups separated largely along taxonomic lines suggesting minimal lateral mdh transfer between Archaea. More... »

PAGES

741-746

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00792-007-0081-2

DOI

http://dx.doi.org/10.1007/s00792-007-0081-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047865542

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17487443


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