Gene cloning, heterologous overexpression and optimized refolding of the NAD-glutamate dehydrogenase from Haloferax mediterranei View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2006-04

AUTHORS

Susana Díaz, Francisco Pérez-Pomares, Carmen Pire, Juan Ferrer, María-José Bonete

ABSTRACT

The NAD-dependent glutamate dehydrogenase (GDH) gene from the halophilic archaeon Haloferax mediterranei has been cloned. The analysis of the nucleotide sequence revealed an open reading frame of 1323 bp that encodes a NAD-GDH. The amino acid sequence displayed high homology with those from other sources, especially the highly conserved residues involved in 2-oxoglutarate binding. The expression of this gene in Escherichia coli, the refolding and further characterization, yielded a fully active NAD-GDH with the same features than those found for the wild-type enzyme. This halophilic NAD-GDH showed a highly dependence on salts for both stability and activity, being essential for the refolding of the recombinant enzyme. More... »

PAGES

105-115

Journal

TITLE

Extremophiles

ISSUE

2

VOLUME

10

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00792-005-0478-8

    DOI

    http://dx.doi.org/10.1007/s00792-005-0478-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1036365512

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/16200391


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