Comparison of two observational methods, scanning electron and confocal laser scanning microscopies, in the adhesive interface analysis of endodontic sealers ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-01-17

AUTHORS

Maybell Tedesco, Marcelo Carvalho Chain, Eduardo Antunes Bortoluzzi, Lucas da Fonseca Roberti Garcia, Ana Maria Hecke Alves, Cleonice Silveira Teixeira

ABSTRACT

ObjectivesTo compare the accuracy of confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) during the analysis of the adhesive interface integrity and intratubular penetration of root canal sealers to radicular dentine.Materials and methodsTwenty roots of human maxillary incisors were prepared and distributed into two groups (n = 10), followed by filling with gutta-percha and Endofill (G1) or AH Plus (G2). After 7 days, roots were sectioned and analyzed under CLSM and SEM. Score systems were used to evaluate the adhesive interface integrity (0–4) and sealer intratubular penetration (0–3). Data were submitted to Wilcoxon-Mann-Whitney and Kendall correlation statistical tests (α = 5%).ResultsIn the adhesive interface analysis, CLSM was similar (P = 0.157) to SEM for Endofill; however, the results were different for AH Plus (P = 0.029). Intratubular penetration had significant difference between observational methods for both sealers (P < 0.0001). Correlation analysis between SEM and CLSM for adhesive interface was moderate for Endofill and low for AH Plus. Intratubular penetration was low for both sealers.ConclusionSEM and CLSM analysis had similar results when sealers were compared, with a more homogeneous adhesive interface, and greater intratubular penetration for AH Plus. Comparison between observational methods demonstrated low positive correlation for adhesive interface and intratubular penetration analysis.Clinical relevanceA proper interface formed between sealer and dentine is very important for final quality of root canal filling. Observational methods which allow an accurate analysis of this interface must be selected to assess such features. More... »

PAGES

2353-2361

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Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00784-018-2336-y

DOI

http://dx.doi.org/10.1007/s00784-018-2336-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1100465570

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29344806


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