The effects of physical activity on apoptosis and lubricin expression in articular cartilage in rats with glucocorticoid-induced osteoporosis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-12-22

AUTHORS

Giuseppe Musumeci, Carla Loreto, Rosalia Leonardi, Sergio Castorina, Salvatore Giunta, Maria Luisa Carnazza, Francesca Maria Trovato, Karin Pichler, Annelie Martina Weinberg

ABSTRACT

Glucocorticoids are considered the most powerful anti-inflammatory and immunomodulating drugs. However, a number of side-effects are well documented in different diseases, including articular cartilage, where increases or decreases in the synthesis of hormone-dependent extracellular matrix components are seen. The objective of this study has been to test the effects of procedures or drugs affecting bone metabolism on articular cartilage in rats with prednisolone-induced osteoporosis and to evaluate the outcomes of physical activity with treadmill and vibration platform training on articular cartilage. The animals were divided into 5 groups, and bone and cartilage evaluations were performed using whole-body scans and histomorphometric analysis. Lubricin and caspase-3 expression were evaluated by immunohistochemistry, Western blot analysis and biochemical analysis. These results confirm the beneficial effect of physical activity on the articular cartilage. The effects of drug therapy with glucocorticoids decrease the expression of lubricin and increase the expression of caspase-3 in the rats, while after physical activity the values return to normal compared to the control group. Our findings suggest that it might be possible that mechanical stimulation in the articular cartilage could induce the expression of lubricin, which is capable of inhibiting caspase-3 activity, preventing chondrocyte death. We can assume that the physiologic balance between lubricin and caspase-3 could maintain the integrity of cartilage. Therefore, in certain diseases such as osteoporosis, mechanical stimulation could be a possible therapeutic treatment. With our results we can propose the hypothesis that physical activity could also be used as a therapeutic treatment for cartilage disease such as osteoarthritis. More... »

PAGES

274-284

References to SciGraph publications

  • 2011-07. Effects of propranolol on the development of glucocorticoid-induced osteoporosis in male rats in PHARMACOLOGICAL REPORTS
  • 2007-07. The role of physical activity in producing and maintaining weight loss in NATURE REVIEWS ENDOCRINOLOGY
  • 2011-08-31. Expression of β-defensin-4 in “an in vivo and ex vivo model” of human osteoarthritic knee meniscus in KNEE SURGERY, SPORTS TRAUMATOLOGY, ARTHROSCOPY
  • 2011-06-23. A proteomic approach for identification and localization of the pericellular components of chondrocytes in HISTOCHEMISTRY AND CELL BIOLOGY
  • 2010-07-20. Characterization of apoptosis in articular cartilage derived from the knee joints of patients with osteoarthritis in KNEE SURGERY, SPORTS TRAUMATOLOGY, ARTHROSCOPY
  • 2008-07-29. Whole-body vibration slows the acquisition of fat in mature female rats in INTERNATIONAL JOURNAL OF OBESITY
  • 2010-01-23. Lubricin: a novel potential biotherapeutic approaches for the treatment of osteoarthritis in MOLECULAR BIOLOGY REPORTS
  • 2005-04. RETRACTED ARTICLE: Effect of whole-body vibration exercise on lumbar bone mineral density, bone turnover, and chronic back pain in post-menopausal osteoporotic women treated with alendronate in AGING CLINICAL AND EXPERIMENTAL RESEARCH
  • 2010-12-31. Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254) in ARTHRITIS RESEARCH & THERAPY
  • 2011-09-24. Comparative high-resolution pQCT analysis of femoral neck indicates different bone mass distribution in osteoporosis and osteoarthritis in OSTEOPOROSIS INTERNATIONAL
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00774-012-0414-9

    DOI

    http://dx.doi.org/10.1007/s00774-012-0414-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1034340464

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/23263781


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