Effect of Escherichia coli-produced recombinant human bone morphogenetic protein 2 on the regeneration of canine segmental ulnar defects View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-11-02

AUTHORS

Yasuji Harada, Takamasa Itoi, Shigeyuki Wakitani, Hiroyuki Irie, Michiko Sakamoto, Dongwei Zhao, Yoshinori Nezu, Takuya Yogo, Yasushi Hara, Masahiro Tagawa

ABSTRACT

Because bone morphogenetic protein 2 gene transfected Escherichia coli (E-BMP-2) produce recombinant human BMP-2 (rhBMP-2) more efficiently than mammalian cells (Chinese hamster ovary [CHO]-BMP-2), they may be a more cost-effective source of rhBMP-2 for clinical use. However, use of E-BMP-2 for regenerating long bones in large animals has not been reported. In the current study, we evaluated the healing efficacy of E-BMP-2 in a canine model. We created 2.5-cm critical-size segmental ulnar defects in test animals, then implanted E-BMP-2 and 700 mg of artificial bone (beta-tricalcium phosphate; β-TCP) into the wounds. We examined the differential effects of 5 E-BMP-2 treatments (0, 35, 140, 560, and 2240 μg) across 5 experimental groups (control, BMP35, BMP140, BMP560, and BMP2240). Radiography and computed tomography were used to observe the regeneration process. The groups in which higher doses of E-BMP-2 were administered (BMP560 and BMP2240) displayed more pronounced bone regeneration; the regenerated tissues connected to the host bone, and the cross-sectional areas of the regenerated bone were larger than those of the originals. The groups in which lower doses of E-BMP-2 were administered (BMP35 and BMP140) experienced relatively less bone regeneration; furthermore, the regenerated tissues failed to connect to the host bone. In these groups, the cross-sectional areas of the regenerated bone were equal to or smaller than those of the originals. No regeneration was observed in the control group. These findings suggest that, like CHO-BMP-2, E-BMP-2 can be used for the regeneration of large defects in long bones and that its clinical use might decrease the cost of bone regeneration treatments. More... »

PAGES

388-399

Journal

TITLE

Journal of Bone and Mineral Metabolism

ISSUE

4

VOLUME

30

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00774-011-0329-x

DOI

http://dx.doi.org/10.1007/s00774-011-0329-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1014332046

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22042292


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44 bone regeneration
45 bone regeneration treatment
46 canine model
47 cells
48 clinical use
49 coli
50 control group
51 cost
52 cost-effective source
53 cross-sectional area
54 current study
55 defects
56 differential effects
57 doses
58 effect
59 efficacy
60 experimental group
61 findings
62 genes
63 group
64 healing efficacy
65 high doses
66 host bone
67 human BMP-2
68 human bone morphogenetic protein-2
69 large animals
70 large defects
71 less bone regeneration
72 long bones
73 low doses
74 mammalian cells
75 model
76 morphogenetic protein-2
77 original
78 process
79 protein 2
80 protein 2 gene
81 recombinant human BMP-2
82 recombinant human bone morphogenetic protein-2
83 regenerated bone
84 regenerated tissue
85 regeneration
86 regeneration process
87 regeneration treatment
88 segmental ulnar defects
89 source
90 study
91 test animals
92 tissue
93 tomography
94 treatment
95 ulnar
96 ulnar defects
97 use
98 wounds
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