The African froghopper Ptyelus flavescens (suborder: Cicadomorpha) contains two novel and one known peptides of the adipokinetic hormone (AKH) family: ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-10

AUTHORS

Gerd Gäde, Petr Šimek, Heather G. Marco

ABSTRACT

The rationale of "green pesticides" in food security is to use information about endogenous hormones of pest insects to make peptide mimetics that will act against the pest insects to alter their behaviour or physiology, while taking care not to harm beneficial insects or other organisms in the food chain. Such "green" insecticides are designed thus, on the basis of neuropeptide ligand-receptor interaction and it is of paramount interest to have finally a mimetic at hand that is harmful only to pest insects. For this concept to work, one has to identify the ligands in pest and beneficial insects. In this study we investigate adipokinetic hormones (AKHs) from a hemipteran source. The most harmful hemipterans on an economic scale are aphids (Hemiptera: Sternorrhyncha: Aphidoidea) of which the AKH is known. Here we identify the AKH complement of a member of a related suborder, the raintree bug or froghopper Ptyelus flavescens (Hemiptera: Cicadomorpha: Cercopoidea). Identification and sequence elucidation of the adipokinetic peptides of this species was achieved by a heterospecific and conspecific trehalose-mobilizing bioassay, and by liquid chromatography coupled to positive electrospray mass spectrometry (LC-ESI-MS) including tandem MS2 spectra obtained by collision-induced dissociation. High resolution MS was employed to distinguish between Gln and Lys residues in the peptides. Three AKHs are discovered in the raintree bug: an octapeptide (Peram-CAH-I: pEVNFSPNW amide) previously known from cockroaches, and two novel decapeptides (Ptyfl-AKH-I: pEINFSTGWGQ amide and Ptyfl-AKH-II: pEINFSTAWGQ amide). The novel peptides were synthesized and the sequence assignments were unequivocally confirmed by co-elution of synthetic peptides and the natural equivalent and by identical MS data of the two forms. A conspecific bioassay in the froghopper describe the endogenous peptide Ptyfl-AKH-I as hypertrehalosemic. In heterologous bioassays the two novel AKHs induce an increase of circulating carbohydrates in cockroaches: Ptyfl-AKH-I is much more active than Ptyfl-AKH-II. Moreover, if the Ile2 in Ptyfl-AKH-II is replaced with a Leu2 residue, biological activity is further diminished. The current data show that the raintree AKH decapeptides differ by four amino acids from the aphid AKH (Acypi-AKH: pEVNFTPTWGQ amide). Therefore, it may be permissible to use the aphid AKH ligand-receptor pair to develop a "green" insecticide to target aphid metabolism. More... »

PAGES

1679-1690

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00726-017-2461-y

DOI

http://dx.doi.org/10.1007/s00726-017-2461-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1090679814

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28710552


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0608", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Zoology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aphids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Insect Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Spectrometry, Mass, Electrospray Ionization", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Cape Town", 
          "id": "https://www.grid.ac/institutes/grid.7836.a", 
          "name": [
            "Department of Biological Sciences, University of Cape Town, Private Bag, 7700, Rondebosch, South Africa"
          ], 
          "type": "Organization"
        }, 
        "familyName": "G\u00e4de", 
        "givenName": "Gerd", 
        "id": "sg:person.0703051001.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703051001.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Biology Centre", 
          "id": "https://www.grid.ac/institutes/grid.418338.5", 
          "name": [
            "Biology Centre, Czech Academy of Sciences, 37005, \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"
          ], 
          "type": "Organization"
        }, 
        "familyName": "\u0160imek", 
        "givenName": "Petr", 
        "id": "sg:person.01003576003.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003576003.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Cape Town", 
          "id": "https://www.grid.ac/institutes/grid.7836.a", 
          "name": [
            "Department of Biological Sciences, University of Cape Town, Private Bag, 7700, Rondebosch, South Africa"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Marco", 
        "givenName": "Heather G.", 
        "id": "sg:person.01105454645.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105454645.29"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/srep28976", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000511650", 
          "https://doi.org/10.1038/srep28976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2003.08.107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000769676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2003.08.107", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000769676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4419-6902-6_8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000783973", 
          "https://doi.org/10.1007/978-1-4419-6902-6_8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4419-6902-6_8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000783973", 
          "https://doi.org/10.1007/978-1-4419-6902-6_8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-0-12-417010-0.00003-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004782703"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.121392.111", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005524595"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/bchm2.1984.365.1.393", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005555593"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ygcen.2008.06.007", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005832314"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-1910(00)00133-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007689354"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.1002005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008491596"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1749-6632.2008.03625.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009749407"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1749-6632.2008.03625.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009749407"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0020-1790(77)90008-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014735989"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0020-1790(77)90008-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014735989"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00726-016-2314-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015166550", 
          "https://doi.org/10.1007/s00726-016-2314-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00726-016-2314-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015166550", 
          "https://doi.org/10.1007/s00726-016-2314-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1910(80)90004-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015320079"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1910(80)90004-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015320079"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-291x(86)90784-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017016501"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pbio.1000313", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018387346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/b978-0-12-385095-9.00028-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019906072"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.peptides.2007.05.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020111097"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ibmb.2015.07.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020535800"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.peptides.2006.11.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022281422"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0106014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024427778"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/srep32789", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025910050", 
          "https://doi.org/10.1038/srep32789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02045455", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029545573", 
          "https://doi.org/10.1007/bf02045455"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02045455", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029545573", 
          "https://doi.org/10.1007/bf02045455"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/bchm3.1994.375.12.803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030726726"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ygcen.2015.09.032", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031146190"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.peptides.2012.01.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032458535"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cbpa.2012.02.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033629476"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ygcen.2012.04.025", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034250979"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/icb/icp019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035050646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/icb/icp019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035050646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-3032.2009.00717.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036102819"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-3032.2009.00717.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036102819"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.ento.49.061802.123354", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037689816"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00726-015-2011-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042525639", 
          "https://doi.org/10.1007/s00726-015-2011-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00726-015-2011-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042525639", 
          "https://doi.org/10.1007/s00726-015-2011-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ps.755", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042827115"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.peptides.2012.02.019", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043044038"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.0307-6962.2004.0375.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044701773"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s12862-016-0621-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053174347", 
          "https://doi.org/10.1186/s12862-016-0621-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ibmb.2017.01.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053843207"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076349527", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/arch.21376", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083856306"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-10", 
    "datePublishedReg": "2017-10-01", 
    "description": "The rationale of \"green pesticides\" in food security is to use information about endogenous hormones of pest insects to make peptide mimetics that will act against the pest insects to alter their behaviour or physiology, while taking care not to harm beneficial insects or other organisms in the food chain. Such \"green\" insecticides are designed thus, on the basis of neuropeptide ligand-receptor interaction and it is of paramount interest to have finally a mimetic at hand that is harmful only to pest insects. For this concept to work, one has to identify the ligands in pest and beneficial insects. In this study we investigate adipokinetic hormones (AKHs) from a hemipteran source. The most harmful hemipterans on an economic scale are aphids (Hemiptera: Sternorrhyncha: Aphidoidea) of which the AKH is known. Here we identify the AKH complement of a member of a related suborder, the raintree bug or froghopper Ptyelus flavescens (Hemiptera: Cicadomorpha: Cercopoidea). Identification and sequence elucidation of the adipokinetic peptides of this species was achieved by a heterospecific and conspecific trehalose-mobilizing bioassay, and by liquid chromatography coupled to positive electrospray mass spectrometry (LC-ESI-MS) including tandem MS2 spectra obtained by collision-induced dissociation. High resolution MS was employed to distinguish between Gln and Lys residues in the peptides. Three AKHs are discovered in the raintree bug: an octapeptide (Peram-CAH-I: pEVNFSPNW amide) previously known from cockroaches, and two novel decapeptides (Ptyfl-AKH-I: pEINFSTGWGQ amide and Ptyfl-AKH-II: pEINFSTAWGQ amide). The novel peptides were synthesized and the sequence assignments were unequivocally confirmed by co-elution of synthetic peptides and the natural equivalent and by identical MS data of the two forms. A conspecific bioassay in the froghopper describe the endogenous peptide Ptyfl-AKH-I as hypertrehalosemic. In heterologous bioassays the two novel AKHs induce an increase of circulating carbohydrates in cockroaches: Ptyfl-AKH-I is much more active than Ptyfl-AKH-II. Moreover, if the Ile2 in Ptyfl-AKH-II is replaced with a Leu2 residue, biological activity is further diminished. The current data show that the raintree AKH decapeptides differ by four amino acids from the aphid AKH (Acypi-AKH: pEVNFTPTWGQ amide). Therefore, it may be permissible to use the aphid AKH ligand-receptor pair to develop a \"green\" insecticide to target aphid metabolism.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00726-017-2461-y", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.6879513", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.7071612", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1101866", 
        "issn": [
          "0939-4451", 
          "1438-2199"
        ], 
        "name": "Amino Acids", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "49"
      }
    ], 
    "name": "The African froghopper Ptyelus flavescens (suborder: Cicadomorpha) contains two novel and one known peptides of the adipokinetic hormone (AKH) family: structure, function and comparison with aphid AKH (suborder: Sternorrhyncha)", 
    "pagination": "1679-1690", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "97bc33cc7c00776c145fa8f102d6f1531d455d01d0efba0ca9cd448ff46be937"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28710552"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9200312"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00726-017-2461-y"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1090679814"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00726-017-2461-y", 
      "https://app.dimensions.ai/details/publication/pub.1090679814"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:28", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000349_0000000349/records_113640_00000004.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs00726-017-2461-y"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00726-017-2461-y'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00726-017-2461-y'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00726-017-2461-y'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00726-017-2461-y'


 

This table displays all metadata directly associated to this object as RDF triples.

230 TRIPLES      21 PREDICATES      72 URIs      26 LITERALS      14 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00726-017-2461-y schema:about N48ee0da6d6fe470a91f6a4c6a6ad859f
2 N58704bcc454b4ebd85a748cae0acd365
3 N6bb861ed556c4461b45b6cb97d612d3e
4 Na54257f0a4914460af4a021b8004b727
5 Nddf11163439c459aab85e18a146ec6ac
6 anzsrc-for:06
7 anzsrc-for:0608
8 schema:author N0752485a036f4652984688cee7bccc43
9 schema:citation sg:pub.10.1007/978-1-4419-6902-6_8
10 sg:pub.10.1007/bf02045455
11 sg:pub.10.1007/s00726-015-2011-4
12 sg:pub.10.1007/s00726-016-2314-0
13 sg:pub.10.1038/srep28976
14 sg:pub.10.1038/srep32789
15 sg:pub.10.1186/s12862-016-0621-4
16 https://app.dimensions.ai/details/publication/pub.1076349527
17 https://doi.org/10.1002/arch.21376
18 https://doi.org/10.1002/ps.755
19 https://doi.org/10.1016/0006-291x(86)90784-9
20 https://doi.org/10.1016/0020-1790(77)90008-7
21 https://doi.org/10.1016/0022-1910(80)90004-9
22 https://doi.org/10.1016/b978-0-12-385095-9.00028-2
23 https://doi.org/10.1016/b978-0-12-417010-0.00003-3
24 https://doi.org/10.1016/j.bbrc.2003.08.107
25 https://doi.org/10.1016/j.cbpa.2012.02.004
26 https://doi.org/10.1016/j.ibmb.2015.07.014
27 https://doi.org/10.1016/j.ibmb.2017.01.005
28 https://doi.org/10.1016/j.peptides.2006.11.019
29 https://doi.org/10.1016/j.peptides.2007.05.004
30 https://doi.org/10.1016/j.peptides.2012.01.009
31 https://doi.org/10.1016/j.peptides.2012.02.019
32 https://doi.org/10.1016/j.ygcen.2008.06.007
33 https://doi.org/10.1016/j.ygcen.2012.04.025
34 https://doi.org/10.1016/j.ygcen.2015.09.032
35 https://doi.org/10.1016/s0022-1910(00)00133-5
36 https://doi.org/10.1093/icb/icp019
37 https://doi.org/10.1101/gr.121392.111
38 https://doi.org/10.1111/j.0307-6962.2004.0375.x
39 https://doi.org/10.1111/j.1365-3032.2009.00717.x
40 https://doi.org/10.1111/j.1749-6632.2008.03625.x
41 https://doi.org/10.1146/annurev.ento.49.061802.123354
42 https://doi.org/10.1371/journal.pbio.1000313
43 https://doi.org/10.1371/journal.pbio.1002005
44 https://doi.org/10.1371/journal.pone.0106014
45 https://doi.org/10.1515/bchm2.1984.365.1.393
46 https://doi.org/10.1515/bchm3.1994.375.12.803
47 schema:datePublished 2017-10
48 schema:datePublishedReg 2017-10-01
49 schema:description The rationale of "green pesticides" in food security is to use information about endogenous hormones of pest insects to make peptide mimetics that will act against the pest insects to alter their behaviour or physiology, while taking care not to harm beneficial insects or other organisms in the food chain. Such "green" insecticides are designed thus, on the basis of neuropeptide ligand-receptor interaction and it is of paramount interest to have finally a mimetic at hand that is harmful only to pest insects. For this concept to work, one has to identify the ligands in pest and beneficial insects. In this study we investigate adipokinetic hormones (AKHs) from a hemipteran source. The most harmful hemipterans on an economic scale are aphids (Hemiptera: Sternorrhyncha: Aphidoidea) of which the AKH is known. Here we identify the AKH complement of a member of a related suborder, the raintree bug or froghopper Ptyelus flavescens (Hemiptera: Cicadomorpha: Cercopoidea). Identification and sequence elucidation of the adipokinetic peptides of this species was achieved by a heterospecific and conspecific trehalose-mobilizing bioassay, and by liquid chromatography coupled to positive electrospray mass spectrometry (LC-ESI-MS) including tandem MS<sup>2</sup> spectra obtained by collision-induced dissociation. High resolution MS was employed to distinguish between Gln and Lys residues in the peptides. Three AKHs are discovered in the raintree bug: an octapeptide (Peram-CAH-I: pEVNFSPNW amide) previously known from cockroaches, and two novel decapeptides (Ptyfl-AKH-I: pEINFSTGWGQ amide and Ptyfl-AKH-II: pEINFSTAWGQ amide). The novel peptides were synthesized and the sequence assignments were unequivocally confirmed by co-elution of synthetic peptides and the natural equivalent and by identical MS data of the two forms. A conspecific bioassay in the froghopper describe the endogenous peptide Ptyfl-AKH-I as hypertrehalosemic. In heterologous bioassays the two novel AKHs induce an increase of circulating carbohydrates in cockroaches: Ptyfl-AKH-I is much more active than Ptyfl-AKH-II. Moreover, if the Ile<sup>2</sup> in Ptyfl-AKH-II is replaced with a Leu<sup>2</sup> residue, biological activity is further diminished. The current data show that the raintree AKH decapeptides differ by four amino acids from the aphid AKH (Acypi-AKH: pEVNFTPTWGQ amide). Therefore, it may be permissible to use the aphid AKH ligand-receptor pair to develop a "green" insecticide to target aphid metabolism.
50 schema:genre research_article
51 schema:inLanguage en
52 schema:isAccessibleForFree false
53 schema:isPartOf N5dcb9defd21b46869f96cdfc5f7cdd8f
54 N5eb0aa5b72724f2d91a38c825733c667
55 sg:journal.1101866
56 schema:name The African froghopper Ptyelus flavescens (suborder: Cicadomorpha) contains two novel and one known peptides of the adipokinetic hormone (AKH) family: structure, function and comparison with aphid AKH (suborder: Sternorrhyncha)
57 schema:pagination 1679-1690
58 schema:productId N339e9b8973b340ea9f46943aedde9b5d
59 N36a187e150a94f1b8e124de35489937f
60 N5f65a1271c444bafae1248c413526c16
61 Naaa465de21ca41e59251ad58be80575b
62 Nbd7f90b857b542b6926a3c7805cbecae
63 schema:sameAs https://app.dimensions.ai/details/publication/pub.1090679814
64 https://doi.org/10.1007/s00726-017-2461-y
65 schema:sdDatePublished 2019-04-11T10:28
66 schema:sdLicense https://scigraph.springernature.com/explorer/license/
67 schema:sdPublisher N7f88b75a2718450cae4452e11ca2f6d5
68 schema:url https://link.springer.com/10.1007%2Fs00726-017-2461-y
69 sgo:license sg:explorer/license/
70 sgo:sdDataset articles
71 rdf:type schema:ScholarlyArticle
72 N0752485a036f4652984688cee7bccc43 rdf:first sg:person.0703051001.95
73 rdf:rest N329b3a01c61b40678a12636a5235aecd
74 N329b3a01c61b40678a12636a5235aecd rdf:first sg:person.01003576003.60
75 rdf:rest N9398f57b28a9403fb043d14a0045ae25
76 N339e9b8973b340ea9f46943aedde9b5d schema:name nlm_unique_id
77 schema:value 9200312
78 rdf:type schema:PropertyValue
79 N36a187e150a94f1b8e124de35489937f schema:name dimensions_id
80 schema:value pub.1090679814
81 rdf:type schema:PropertyValue
82 N48ee0da6d6fe470a91f6a4c6a6ad859f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name Aphids
84 rdf:type schema:DefinedTerm
85 N58704bcc454b4ebd85a748cae0acd365 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Animals
87 rdf:type schema:DefinedTerm
88 N5dcb9defd21b46869f96cdfc5f7cdd8f schema:issueNumber 10
89 rdf:type schema:PublicationIssue
90 N5eb0aa5b72724f2d91a38c825733c667 schema:volumeNumber 49
91 rdf:type schema:PublicationVolume
92 N5f65a1271c444bafae1248c413526c16 schema:name doi
93 schema:value 10.1007/s00726-017-2461-y
94 rdf:type schema:PropertyValue
95 N6bb861ed556c4461b45b6cb97d612d3e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Spectrometry, Mass, Electrospray Ionization
97 rdf:type schema:DefinedTerm
98 N7f88b75a2718450cae4452e11ca2f6d5 schema:name Springer Nature - SN SciGraph project
99 rdf:type schema:Organization
100 N9398f57b28a9403fb043d14a0045ae25 rdf:first sg:person.01105454645.29
101 rdf:rest rdf:nil
102 Na54257f0a4914460af4a021b8004b727 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Insect Proteins
104 rdf:type schema:DefinedTerm
105 Naaa465de21ca41e59251ad58be80575b schema:name pubmed_id
106 schema:value 28710552
107 rdf:type schema:PropertyValue
108 Nbd7f90b857b542b6926a3c7805cbecae schema:name readcube_id
109 schema:value 97bc33cc7c00776c145fa8f102d6f1531d455d01d0efba0ca9cd448ff46be937
110 rdf:type schema:PropertyValue
111 Nddf11163439c459aab85e18a146ec6ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Peptides
113 rdf:type schema:DefinedTerm
114 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
115 schema:name Biological Sciences
116 rdf:type schema:DefinedTerm
117 anzsrc-for:0608 schema:inDefinedTermSet anzsrc-for:
118 schema:name Zoology
119 rdf:type schema:DefinedTerm
120 sg:grant.6879513 http://pending.schema.org/fundedItem sg:pub.10.1007/s00726-017-2461-y
121 rdf:type schema:MonetaryGrant
122 sg:grant.7071612 http://pending.schema.org/fundedItem sg:pub.10.1007/s00726-017-2461-y
123 rdf:type schema:MonetaryGrant
124 sg:journal.1101866 schema:issn 0939-4451
125 1438-2199
126 schema:name Amino Acids
127 rdf:type schema:Periodical
128 sg:person.01003576003.60 schema:affiliation https://www.grid.ac/institutes/grid.418338.5
129 schema:familyName Šimek
130 schema:givenName Petr
131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01003576003.60
132 rdf:type schema:Person
133 sg:person.01105454645.29 schema:affiliation https://www.grid.ac/institutes/grid.7836.a
134 schema:familyName Marco
135 schema:givenName Heather G.
136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01105454645.29
137 rdf:type schema:Person
138 sg:person.0703051001.95 schema:affiliation https://www.grid.ac/institutes/grid.7836.a
139 schema:familyName Gäde
140 schema:givenName Gerd
141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703051001.95
142 rdf:type schema:Person
143 sg:pub.10.1007/978-1-4419-6902-6_8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000783973
144 https://doi.org/10.1007/978-1-4419-6902-6_8
145 rdf:type schema:CreativeWork
146 sg:pub.10.1007/bf02045455 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029545573
147 https://doi.org/10.1007/bf02045455
148 rdf:type schema:CreativeWork
149 sg:pub.10.1007/s00726-015-2011-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042525639
150 https://doi.org/10.1007/s00726-015-2011-4
151 rdf:type schema:CreativeWork
152 sg:pub.10.1007/s00726-016-2314-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015166550
153 https://doi.org/10.1007/s00726-016-2314-0
154 rdf:type schema:CreativeWork
155 sg:pub.10.1038/srep28976 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000511650
156 https://doi.org/10.1038/srep28976
157 rdf:type schema:CreativeWork
158 sg:pub.10.1038/srep32789 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025910050
159 https://doi.org/10.1038/srep32789
160 rdf:type schema:CreativeWork
161 sg:pub.10.1186/s12862-016-0621-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053174347
162 https://doi.org/10.1186/s12862-016-0621-4
163 rdf:type schema:CreativeWork
164 https://app.dimensions.ai/details/publication/pub.1076349527 schema:CreativeWork
165 https://doi.org/10.1002/arch.21376 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083856306
166 rdf:type schema:CreativeWork
167 https://doi.org/10.1002/ps.755 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042827115
168 rdf:type schema:CreativeWork
169 https://doi.org/10.1016/0006-291x(86)90784-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017016501
170 rdf:type schema:CreativeWork
171 https://doi.org/10.1016/0020-1790(77)90008-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014735989
172 rdf:type schema:CreativeWork
173 https://doi.org/10.1016/0022-1910(80)90004-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015320079
174 rdf:type schema:CreativeWork
175 https://doi.org/10.1016/b978-0-12-385095-9.00028-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019906072
176 rdf:type schema:CreativeWork
177 https://doi.org/10.1016/b978-0-12-417010-0.00003-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004782703
178 rdf:type schema:CreativeWork
179 https://doi.org/10.1016/j.bbrc.2003.08.107 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000769676
180 rdf:type schema:CreativeWork
181 https://doi.org/10.1016/j.cbpa.2012.02.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033629476
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1016/j.ibmb.2015.07.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020535800
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1016/j.ibmb.2017.01.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053843207
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1016/j.peptides.2006.11.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022281422
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/j.peptides.2007.05.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020111097
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/j.peptides.2012.01.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032458535
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/j.peptides.2012.02.019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043044038
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/j.ygcen.2008.06.007 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005832314
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/j.ygcen.2012.04.025 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034250979
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/j.ygcen.2015.09.032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031146190
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/s0022-1910(00)00133-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007689354
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1093/icb/icp019 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035050646
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1101/gr.121392.111 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005524595
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1111/j.0307-6962.2004.0375.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044701773
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1111/j.1365-3032.2009.00717.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1036102819
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1111/j.1749-6632.2008.03625.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009749407
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1146/annurev.ento.49.061802.123354 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037689816
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1371/journal.pbio.1000313 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018387346
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1371/journal.pbio.1002005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008491596
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1371/journal.pone.0106014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024427778
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1515/bchm2.1984.365.1.393 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005555593
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1515/bchm3.1994.375.12.803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030726726
224 rdf:type schema:CreativeWork
225 https://www.grid.ac/institutes/grid.418338.5 schema:alternateName Biology Centre
226 schema:name Biology Centre, Czech Academy of Sciences, 37005, České Budějovice, Czech Republic
227 rdf:type schema:Organization
228 https://www.grid.ac/institutes/grid.7836.a schema:alternateName University of Cape Town
229 schema:name Department of Biological Sciences, University of Cape Town, Private Bag, 7700, Rondebosch, South Africa
230 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...