Anti-cancer labdane diterpenoids from adventitious roots of Andrographis paniculata: augmentation of production prospect endowed with pathway gene expression View Full Text


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Article Info

DATE

2018-03-20

AUTHORS

Sailendra Singh, Pallavi Pandey, Sumit Ghosh, Suchitra Banerjee

ABSTRACT

Andrographolide (AD) is the time-honoured pharmacologically active constituent of the traditionally renowned medicinal plant—Andrographis paniculata. Advancements in the target-oriented drug discovery process have further unravelled the immense therapeutic credibility of another unique molecule—neoandrographolide (NAD). The escalated market demand of these anti-cancer diterpenes is increasingly facing unrelenting hurdles of demand and supply disparity, attributable to their limited yield. Callus and adventitious root cultures were generated to explore their biosynthetic potentials which first time revealed NAD production along with AD. Optimization of the types and concentrations of auxins along with media form and cultivation time led to the successful tuning towards establishing adventitious roots as a superior production alternative for both AD/NAD. Supplementation of IBA to the NAA + Kn-containing MS medium boosted the overall growth and AD/NAD synthesis in the adventitious roots. Compared to control leaves, the adventitious root exhibited about 2.61- and 8.8-fold higher contents of AD and NAD, respectively. The qRT-PCR involving nine key pathway genes was studied, which revealed upregulation of GGPS1 and HMGR1/2 genes and downregulation of DXS1/2 and HDR1/2 genes in the adventitious root as compared to that in the control leaves. Such observations highlight that in vitro cultures can serve as efficient production alternatives for AD/NAD as the cytosolic genes (HMGR1/2 of MVA pathway) are competent enough to take over from the plastidial genes (DXS1/2 and HDR1/2 of MEP pathway), provided the accredited first branch-point regulatory gene (GGPS) expression and the culture requirements are optimally fulfilled. More... »

PAGES

1387-1400

References to SciGraph publications

  • 2015-06-23. Effect of PGRs in adventitious root culture in vitro: present scenario and future prospects in RENDICONTI LINCEI. SCIENZE FISICHE E NATURALI
  • 2011-10-11. Exudation: an expanding technique for continuous production and release of secondary metabolites from plant cell suspension and hairy root cultures in PLANT CELL REPORTS
  • 2012-09-23. Elicitation of Andrographolide in the Suspension Cultures of Andrographis paniculata in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2015-09-02. Andrographis paniculata transcriptome provides molecular insights into tissue-specific accumulation of medicinal diterpenes in BMC GENOMICS
  • 2014-12-11. Tools for biotechnological production of useful phytochemicals from adventitious root cultures in PHYTOCHEMISTRY REVIEWS
  • 2016-10-03. Anticancer potential of labdane diterpenoid lactone “andrographolide” and its derivatives: a semi-synthetic approach in PHYTOCHEMISTRY REVIEWS
  • 2011-10-02. A bitter plant with a sweet future? A comprehensive review of an oriental medicinal plant: Andrographis paniculata in PHYTOCHEMISTRY REVIEWS
  • 2016-10-20. Enhanced diterpene lactone (andrographolide) production from elicited adventitious root cultures of Andrographis paniculata in RESEARCH ON CHEMICAL INTERMEDIATES
  • 2015-09-18. Activity of andrographolide against chikungunya virus infection in SCIENTIFIC REPORTS
  • 2014-10-12. Production of anti-cancer triterpene (betulinic acid) from callus cultures of different Ocimum species and its elicitation in PROTOPLASMA
  • 2017-01-18. Impacts of methyl jasmonate and phenyl acetic acid on biomass accumulation and antioxidant potential in adventitious roots of Ajuga bracteosa Wall ex Benth., a high valued endangered medicinal plant in PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
  • 2003-03. Lateral root development and saponin accumulation as affected by IBA or NAA in adventitious root cultures of Panax ginseng C.A. Meyer in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 2015-05-14. Multiple shoot induction and jasmonic versus salicylic acid driven elicitation for enhanced andrographolide production in Andrographis paniculata in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 2013-10-24. Enhanced synthesis of andrographolide by Aspergillus niger and Penicillium expansum elicitors in cell suspension culture of Andrographis paniculata (Burm. f.) Nees in BOTANICAL STUDIES
  • 2014-08-16. Design of Bioreactors for Plant Cell and Organ Cultures in PRODUCTION OF BIOMASS AND BIOACTIVE COMPOUNDS USING BIOREACTOR TECHNOLOGY
  • 2013-01-15. Changes in the contents of four active diterpenoids at different growth stages in Andrographis paniculata (Burm.f.) Nees (Chuanxinlian) in CHINESE MEDICINE
  • 2014-06-12. Enhanced secondary metabolite production and pathway gene expression by leaf explants-induced direct root morphotypes are regulated by combination of growth regulators and culture conditions in Centella asiatica (L.) urban in PLANT GROWTH REGULATION
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    http://scigraph.springernature.com/pub.10.1007/s00709-018-1211-7

    DOI

    http://dx.doi.org/10.1007/s00709-018-1211-7

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1101635655

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/29560544


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    44 NAD synthesis
    45 active constituents
    46 advancement
    47 adventitious root cultures
    48 adventitious roots
    49 alternative
    50 andrographolide
    51 augmentation
    52 auxin
    53 biosynthetic potential
    54 concentration
    55 concentration of auxin
    56 constituents
    57 content
    58 control
    59 credibility
    60 cultivation time
    61 culture
    62 culture requirements
    63 cytosolic genes
    64 demand
    65 discovery process
    66 disparities
    67 diterpenes
    68 diterpenoids
    69 downregulation
    70 drug discovery process
    71 efficient production alternatives
    72 expression
    73 first time
    74 form
    75 gene expression
    76 genes
    77 growth
    78 high content
    79 hurdles
    80 key pathway genes
    81 labdane diterpenoids
    82 leaves
    83 limited yield
    84 market demand
    85 media forms
    86 medicinal plant Andrographis paniculata
    87 medium
    88 molecules
    89 observations
    90 optimization
    91 overall growth
    92 paniculata
    93 pathway gene expression
    94 pathway genes
    95 plant Andrographis paniculata
    96 plastidial genes
    97 potential
    98 process
    99 production
    100 production alternatives
    101 production prospects
    102 prospects
    103 qRT-PCR
    104 regulatory gene expression
    105 requirements
    106 root cultures
    107 roots
    108 successful tuning
    109 such observations
    110 supplementation
    111 synthesis
    112 time
    113 tuning
    114 types
    115 unique molecules
    116 upregulation
    117 yield
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