Nitrogen treatment enhances sterols and withaferin A through transcriptional activation of jasmonate pathway, WRKY transcription factors, and biosynthesis genes in ... View Full Text


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Article Info

DATE

2016-03-12

AUTHORS

Shaifali Pal, Akhilesh Kumar Yadav, Anup Kumar Singh, Shubhra Rastogi, Madan Mohan Gupta, Rajesh Kumar Verma, Dinesh A. Nagegowda, Anirban Pal, Ajit Kumar Shasany

ABSTRACT

The medicinal plant Withania somnifera is researched extensively to increase the quantity of withanolides and specifically withaferin A, which finds implications in many pharmacological activities. Due to insufficient knowledge on biosynthesis and unacceptability of transgenic approach, it is preferred to follow alternative physiological methods to increase the yield of withanolides. Prior use of elicitors like salicylic acid, methyl jasmonate, fungal extracts, and even mechanical wounding have shown to increase the withanolide biosynthesis with limited success; however, the commercial viability and logistics of application are debatable. In this investigation, we tested the simple nitrogeneous fertilizers pertaining to the enhancement of withaferin A biosynthesis. Application of ammonium sulfate improved the sterol contents required for the withanolide biosynthesis and correlated to higher expression of pathway genes like FPPS, SMT1, SMT2, SMO1, SMO2, and ODM. Increased expression of a gene homologous to allene oxide cyclase, crucial in jasmonic acid biosynthetic pathway, suggested the involvement of jasmonate signaling. High levels of WRKY gene transcripts indicated transcriptional regulation of the pathway genes. Increase in transcript level could be correlated with a corresponding increase in the protein levels for WsSMT1 and WsWRKY1. The withaferin A increase was also demonstrated in the potted plants growing in the glasshouse and in the open field. These results implicated simple physiological management of nitrogen fertilizer signal to improve the yield of secondary metabolite through probable involvement of jasmonate signal and WRKY transcription factor for the first time, in W. somnifera besides improving the foliage. More... »

PAGES

389-399

References to SciGraph publications

  • 2014-06-20. Analyses of Catharanthus roseus and Arabidopsis thaliana WRKY transcription factors reveal involvement in jasmonate signaling in BMC GENOMICS
  • 2012-10-13. Molecular characterization and promoter analysis of squalene epoxidase gene from Withania somnifera (L.) Dunal in MOLECULAR BIOLOGY REPORTS
  • 2012-04-15. Cloning and characterization of 2-C-methyl-d-erythritol-4-phosphate pathway genes for isoprenoid biosynthesis from Indian ginseng, Withania somnifera in PROTOPLASMA
  • 2003-04. Jasmonate biosynthesis and the allene oxide cyclase family of Arabidopsis thaliana in PLANT MOLECULAR BIOLOGY
  • 2012-08-31. Cloning and functional characterization of 3-hydroxy-3-methylglutaryl coenzyme A reductase gene from Withania somnifera: an important medicinal plant in PROTOPLASMA
  • 2012-07-28. Optimization of Elicitation Conditions with Methyl Jasmonate and Salicylic Acid to Improve the Productivity of Withanolides in the Adventitious Root Culture of Withania somnifera (L.) Dunal in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2009-12-20. DMSO triggers the generation of ROS leading to an increase in artemisinin and dihydroartemisinic acid in Artemisia annua shoot cultures in PLANT CELL REPORTS
  • 2011-03-22. Differential expression of farnesyl diphosphate synthase gene from Withania somnifera in different chemotypes and in response to elicitors in PLANT GROWTH REGULATION
  • 2009-12-11. Agrobacterium tumefaciens-mediated transformation of Withania somnifera (L.) Dunal: an important medicinal plant in PLANT CELL REPORTS
  • 2013-02-02. Increased production of withanolide A, withanone, and withaferin A in hairy root cultures of Withania somnifera (L.) Dunal elicited with methyl jasmonate and salicylic acid in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00709-016-0959-x

    DOI

    http://dx.doi.org/10.1007/s00709-016-0959-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1047327415

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26971099


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