Influence of cellular differentiation and elicitation on intermediate and late steps of terpenoid indole alkaloid biosynthesis in Catharanthus roseus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2010-03-09

AUTHORS

Ashutosh K. Shukla, Ajit K. Shasany, Ram K. Verma, Madan M. Gupta, Ajay K. Mathur, Suman P. S. Khanuja

ABSTRACT

The invaluable antineoplastic bisindole alkaloids of Catharanthus roseus and their precursor, vindoline, are not produced in cell cultures. The intricacies involved in endogenous (cellular differentiation) and exogenous (elicitation) regulation of their biosynthesis need to be dissected out for favorable exploitation. This study aimed at elucidating the effect of Pythium aphanidermatum homogenate and methyl jasmonate (MeJa) on in vitro cultures (of cv. ‘Dhawal’) representing increasing level of differentiation (suspension < callus < shoots) in terms of alkaloid accumulation and transcript abundance of strictosidine β-d-glucosidase (SGD) and acetyl-CoA: 4-O-deacetylvindoline 4-O-acetyl-transferase (DAT) genes, representing intermediate and late steps, respectively, of terpenoid indole alkaloid biosynthesis. Elicitation of suspension cultures caused transcriptional upregulation of SGD and enhanced the accumulation of total alkaloids but did not produce vindoline as DAT transcripts were always found to be absent in suspension-cultured cells. Vindoline was also not detected in unelicited and MeJa-treated callus but appeared upon elicitation with fungal homogenate for 24 h that coincided with maximal DAT transcription. Transcript levels of both genes increased upon elicitation of callus but remained below levels present in the mature plant leaf. Elicitation caused appearance of vindoline in shoots and increased the transcript abundance of both genes beyond levels observed in the mature plant leaf. Differentiation was essential for expression of DAT but not SGD, and vindoline biosynthetic potential increased with it. More... »

PAGES

35-47

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00709-010-0120-1

DOI

http://dx.doi.org/10.1007/s00709-010-0120-1

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1010206130

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/20217156


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biosynthetic Pathways", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Catharanthus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pythium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Messenger", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Reverse Transcriptase Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Secologanin Tryptamine Alkaloids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tissue Culture Techniques", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shukla", 
        "givenName": "Ashutosh K.", 
        "id": "sg:person.0746716663.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746716663.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shasany", 
        "givenName": "Ajit K.", 
        "id": "sg:person.01207061437.96", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207061437.96"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Verma", 
        "givenName": "Ram K.", 
        "id": "sg:person.01212430255.34", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01212430255.34"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gupta", 
        "givenName": "Madan M.", 
        "id": "sg:person.01242460441.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242460441.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Plant Tissue Culture Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India", 
          "id": "http://www.grid.ac/institutes/grid.417631.6", 
          "name": [
            "Plant Tissue Culture Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mathur", 
        "givenName": "Ajay K.", 
        "id": "sg:person.01047432137.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047432137.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "C41-42, Double Storey, Ramesh Nagar, 110015, New Delhi, India", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India", 
            "C41-42, Double Storey, Ramesh Nagar, 110015, New Delhi, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Khanuja", 
        "givenName": "Suman P. S.", 
        "id": "sg:person.010601233534.89", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010601233534.89"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1385/mb:22:1:001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007314022", 
          "https://doi.org/10.1385/mb:22:1:001"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/b:bile.0000025879.53632.f2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039737788", 
          "https://doi.org/10.1023/b:bile.0000025879.53632.f2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nchembio.160", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017938264", 
          "https://doi.org/10.1038/nchembio.160"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/b:bile.0000023028.21985.07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028991840", 
          "https://doi.org/10.1023/b:bile.0000023028.21985.07"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10529-008-9881-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000020253", 
          "https://doi.org/10.1007/s10529-008-9881-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/a:1026526522555", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035254423", 
          "https://doi.org/10.1023/a:1026526522555"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00425-004-1380-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037908902", 
          "https://doi.org/10.1007/s00425-004-1380-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00043397", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050583998", 
          "https://doi.org/10.1007/bf00043397"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2010-03-09", 
    "datePublishedReg": "2010-03-09", 
    "description": "The invaluable antineoplastic bisindole alkaloids of Catharanthus roseus and their precursor, vindoline, are not produced in cell cultures. The intricacies involved in endogenous (cellular differentiation) and exogenous (elicitation) regulation of their biosynthesis need to be dissected out for favorable exploitation. This study aimed at elucidating the effect of Pythium aphanidermatum homogenate and methyl jasmonate (MeJa) on in vitro cultures (of cv. \u2018Dhawal\u2019) representing increasing level of differentiation (suspension < callus < shoots) in terms of alkaloid accumulation and transcript abundance of strictosidine \u03b2-d-glucosidase (SGD) and acetyl-CoA: 4-O-deacetylvindoline 4-O-acetyl-transferase (DAT) genes, representing intermediate and late steps, respectively, of terpenoid indole alkaloid biosynthesis. Elicitation of suspension cultures caused transcriptional upregulation of SGD and enhanced the accumulation of total alkaloids but did not produce vindoline as DAT transcripts were always found to be absent in suspension-cultured cells. Vindoline was also not detected in unelicited and MeJa-treated callus but appeared upon elicitation with fungal homogenate for 24\u00a0h that coincided with maximal DAT transcription. Transcript levels of both genes increased upon elicitation of callus but remained below levels present in the mature plant leaf. Elicitation caused appearance of vindoline in shoots and increased the transcript abundance of both genes beyond levels observed in the mature plant leaf. Differentiation was essential for expression of DAT but not SGD, and vindoline biosynthetic potential increased with it.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s00709-010-0120-1", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1118135", 
        "issn": [
          "0033-183X", 
          "1615-6102"
        ], 
        "name": "Protoplasma", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1-4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "242"
      }
    ], 
    "keywords": [
      "terpenoid indole alkaloid biosynthesis", 
      "indole alkaloid biosynthesis", 
      "mature plant leaves", 
      "alkaloid biosynthesis", 
      "transcript abundance", 
      "plant leaves", 
      "Catharanthus roseus", 
      "later steps", 
      "suspension-cultured cells", 
      "strictosidine \u03b2", 
      "DAT transcripts", 
      "level of differentiation", 
      "fungal homogenate", 
      "biosynthetic potential", 
      "methyl jasmonate", 
      "cellular differentiation", 
      "transcriptional upregulation", 
      "transcript levels", 
      "exogenous regulation", 
      "alkaloid accumulation", 
      "biosynthesis", 
      "suspension cultures", 
      "genes", 
      "expression of DAT", 
      "vindoline", 
      "differentiation", 
      "roseus", 
      "leaves", 
      "cell cultures", 
      "callus", 
      "abundance", 
      "jasmonate", 
      "MeJA", 
      "accumulation", 
      "transcription", 
      "bisindole alkaloids", 
      "transcripts", 
      "shoots", 
      "regulation", 
      "total alkaloids", 
      "culture", 
      "expression", 
      "alkaloids", 
      "upregulation", 
      "glucosidase", 
      "cells", 
      "homogenates", 
      "levels", 
      "elicitation", 
      "precursors", 
      "step", 
      "SGD", 
      "exploitation", 
      "intricacies", 
      "potential", 
      "DAT", 
      "appearance", 
      "study", 
      "effect", 
      "influence", 
      "terms", 
      "invaluable antineoplastic bisindole alkaloids", 
      "antineoplastic bisindole alkaloids", 
      "favorable exploitation", 
      "Pythium aphanidermatum homogenate", 
      "aphanidermatum homogenate", 
      "maximal DAT transcription", 
      "DAT transcription", 
      "elicitation of callus", 
      "appearance of vindoline", 
      "vindoline biosynthetic potential"
    ], 
    "name": "Influence of cellular differentiation and elicitation on intermediate and late steps of terpenoid indole alkaloid biosynthesis in Catharanthus roseus", 
    "pagination": "35-47", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1010206130"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00709-010-0120-1"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "20217156"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00709-010-0120-1", 
      "https://app.dimensions.ai/details/publication/pub.1010206130"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:23", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_504.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s00709-010-0120-1"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00709-010-0120-1'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00709-010-0120-1'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00709-010-0120-1'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00709-010-0120-1'


 

This table displays all metadata directly associated to this object as RDF triples.

252 TRIPLES      22 PREDICATES      116 URIs      99 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00709-010-0120-1 schema:about N15aa4df33da941cea196480d908533c0
2 N2b97d68045884b468b7af57c296afe23
3 N511aca4a00ca46ce9a294a7f14219f7f
4 N7b2774258cde4bcda5d50ca7fbe3581f
5 N807b07eb9451474da2322a6f1d7ed3d3
6 N886679da6a494c0b9ecfeaf621b26ca3
7 N99e6ec9976ba4be9afa0a9340f8fc68b
8 Nd3212ba3dc18422cb9d98a81d195b4ba
9 Nd960a0d494f04b04811787ba3d638039
10 Nf7fce1e79f6b448a8bc1475e6bee841c
11 anzsrc-for:06
12 anzsrc-for:0601
13 anzsrc-for:0604
14 schema:author Naa06b6aa50d44bb19d0085ca51e2c169
15 schema:citation sg:pub.10.1007/bf00043397
16 sg:pub.10.1007/s00425-004-1380-9
17 sg:pub.10.1007/s10529-008-9881-4
18 sg:pub.10.1023/a:1026526522555
19 sg:pub.10.1023/b:bile.0000023028.21985.07
20 sg:pub.10.1023/b:bile.0000025879.53632.f2
21 sg:pub.10.1038/nchembio.160
22 sg:pub.10.1385/mb:22:1:001
23 schema:datePublished 2010-03-09
24 schema:datePublishedReg 2010-03-09
25 schema:description The invaluable antineoplastic bisindole alkaloids of Catharanthus roseus and their precursor, vindoline, are not produced in cell cultures. The intricacies involved in endogenous (cellular differentiation) and exogenous (elicitation) regulation of their biosynthesis need to be dissected out for favorable exploitation. This study aimed at elucidating the effect of Pythium aphanidermatum homogenate and methyl jasmonate (MeJa) on in vitro cultures (of cv. ‘Dhawal’) representing increasing level of differentiation (suspension < callus < shoots) in terms of alkaloid accumulation and transcript abundance of strictosidine β-d-glucosidase (SGD) and acetyl-CoA: 4-O-deacetylvindoline 4-O-acetyl-transferase (DAT) genes, representing intermediate and late steps, respectively, of terpenoid indole alkaloid biosynthesis. Elicitation of suspension cultures caused transcriptional upregulation of SGD and enhanced the accumulation of total alkaloids but did not produce vindoline as DAT transcripts were always found to be absent in suspension-cultured cells. Vindoline was also not detected in unelicited and MeJa-treated callus but appeared upon elicitation with fungal homogenate for 24 h that coincided with maximal DAT transcription. Transcript levels of both genes increased upon elicitation of callus but remained below levels present in the mature plant leaf. Elicitation caused appearance of vindoline in shoots and increased the transcript abundance of both genes beyond levels observed in the mature plant leaf. Differentiation was essential for expression of DAT but not SGD, and vindoline biosynthetic potential increased with it.
26 schema:genre article
27 schema:inLanguage en
28 schema:isAccessibleForFree false
29 schema:isPartOf Na3f574545c73494e95c359a1a801a666
30 Nef248bb073084f3e91086b1675dda17c
31 sg:journal.1118135
32 schema:keywords Catharanthus roseus
33 DAT
34 DAT transcription
35 DAT transcripts
36 MeJA
37 Pythium aphanidermatum homogenate
38 SGD
39 abundance
40 accumulation
41 alkaloid accumulation
42 alkaloid biosynthesis
43 alkaloids
44 antineoplastic bisindole alkaloids
45 aphanidermatum homogenate
46 appearance
47 appearance of vindoline
48 biosynthesis
49 biosynthetic potential
50 bisindole alkaloids
51 callus
52 cell cultures
53 cells
54 cellular differentiation
55 culture
56 differentiation
57 effect
58 elicitation
59 elicitation of callus
60 exogenous regulation
61 exploitation
62 expression
63 expression of DAT
64 favorable exploitation
65 fungal homogenate
66 genes
67 glucosidase
68 homogenates
69 indole alkaloid biosynthesis
70 influence
71 intricacies
72 invaluable antineoplastic bisindole alkaloids
73 jasmonate
74 later steps
75 leaves
76 level of differentiation
77 levels
78 mature plant leaves
79 maximal DAT transcription
80 methyl jasmonate
81 plant leaves
82 potential
83 precursors
84 regulation
85 roseus
86 shoots
87 step
88 strictosidine β
89 study
90 suspension cultures
91 suspension-cultured cells
92 terms
93 terpenoid indole alkaloid biosynthesis
94 total alkaloids
95 transcript abundance
96 transcript levels
97 transcription
98 transcriptional upregulation
99 transcripts
100 upregulation
101 vindoline
102 vindoline biosynthetic potential
103 schema:name Influence of cellular differentiation and elicitation on intermediate and late steps of terpenoid indole alkaloid biosynthesis in Catharanthus roseus
104 schema:pagination 35-47
105 schema:productId N33dff381034a4a088cae0e2885eb47f2
106 Nadfa9e9e5cfe435fb8269f8321625074
107 Nd8dc008fb8dd41a7bfcdfb8325b9c786
108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010206130
109 https://doi.org/10.1007/s00709-010-0120-1
110 schema:sdDatePublished 2021-12-01T19:23
111 schema:sdLicense https://scigraph.springernature.com/explorer/license/
112 schema:sdPublisher N76c18300e94744588a25fc50aaecc83a
113 schema:url https://doi.org/10.1007/s00709-010-0120-1
114 sgo:license sg:explorer/license/
115 sgo:sdDataset articles
116 rdf:type schema:ScholarlyArticle
117 N15aa4df33da941cea196480d908533c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Biosynthetic Pathways
119 rdf:type schema:DefinedTerm
120 N22a4c850d3b743f28b0344a3a22bff28 rdf:first sg:person.01242460441.07
121 rdf:rest N241a4f254ae04edf8d797785e846bd5d
122 N241a4f254ae04edf8d797785e846bd5d rdf:first sg:person.01047432137.18
123 rdf:rest N58d2136a72aa40c9883d495eb7990d54
124 N2b97d68045884b468b7af57c296afe23 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Cell Differentiation
126 rdf:type schema:DefinedTerm
127 N33dff381034a4a088cae0e2885eb47f2 schema:name doi
128 schema:value 10.1007/s00709-010-0120-1
129 rdf:type schema:PropertyValue
130 N511aca4a00ca46ce9a294a7f14219f7f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Gene Expression Regulation, Plant
132 rdf:type schema:DefinedTerm
133 N58d2136a72aa40c9883d495eb7990d54 rdf:first sg:person.010601233534.89
134 rdf:rest rdf:nil
135 N76c18300e94744588a25fc50aaecc83a schema:name Springer Nature - SN SciGraph project
136 rdf:type schema:Organization
137 N7b2774258cde4bcda5d50ca7fbe3581f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name RNA, Messenger
139 rdf:type schema:DefinedTerm
140 N807b07eb9451474da2322a6f1d7ed3d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Pythium
142 rdf:type schema:DefinedTerm
143 N86da6da72e774ed2a0976dc3d10ad54b rdf:first sg:person.01212430255.34
144 rdf:rest N22a4c850d3b743f28b0344a3a22bff28
145 N886679da6a494c0b9ecfeaf621b26ca3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Catharanthus
147 rdf:type schema:DefinedTerm
148 N99e6ec9976ba4be9afa0a9340f8fc68b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Secologanin Tryptamine Alkaloids
150 rdf:type schema:DefinedTerm
151 Na3f574545c73494e95c359a1a801a666 schema:issueNumber 1-4
152 rdf:type schema:PublicationIssue
153 Naa06b6aa50d44bb19d0085ca51e2c169 rdf:first sg:person.0746716663.99
154 rdf:rest Ndcdc271688364b07b3159252e13e150e
155 Nadfa9e9e5cfe435fb8269f8321625074 schema:name dimensions_id
156 schema:value pub.1010206130
157 rdf:type schema:PropertyValue
158 Nd3212ba3dc18422cb9d98a81d195b4ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Tissue Culture Techniques
160 rdf:type schema:DefinedTerm
161 Nd8dc008fb8dd41a7bfcdfb8325b9c786 schema:name pubmed_id
162 schema:value 20217156
163 rdf:type schema:PropertyValue
164 Nd960a0d494f04b04811787ba3d638039 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Reverse Transcriptase Polymerase Chain Reaction
166 rdf:type schema:DefinedTerm
167 Ndcdc271688364b07b3159252e13e150e rdf:first sg:person.01207061437.96
168 rdf:rest N86da6da72e774ed2a0976dc3d10ad54b
169 Nef248bb073084f3e91086b1675dda17c schema:volumeNumber 242
170 rdf:type schema:PublicationVolume
171 Nf7fce1e79f6b448a8bc1475e6bee841c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Plant Proteins
173 rdf:type schema:DefinedTerm
174 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
175 schema:name Biological Sciences
176 rdf:type schema:DefinedTerm
177 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
178 schema:name Biochemistry and Cell Biology
179 rdf:type schema:DefinedTerm
180 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
181 schema:name Genetics
182 rdf:type schema:DefinedTerm
183 sg:journal.1118135 schema:issn 0033-183X
184 1615-6102
185 schema:name Protoplasma
186 schema:publisher Springer Nature
187 rdf:type schema:Periodical
188 sg:person.01047432137.18 schema:affiliation grid-institutes:grid.417631.6
189 schema:familyName Mathur
190 schema:givenName Ajay K.
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047432137.18
192 rdf:type schema:Person
193 sg:person.010601233534.89 schema:affiliation grid-institutes:None
194 schema:familyName Khanuja
195 schema:givenName Suman P. S.
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010601233534.89
197 rdf:type schema:Person
198 sg:person.01207061437.96 schema:affiliation grid-institutes:grid.417631.6
199 schema:familyName Shasany
200 schema:givenName Ajit K.
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207061437.96
202 rdf:type schema:Person
203 sg:person.01212430255.34 schema:affiliation grid-institutes:grid.417631.6
204 schema:familyName Verma
205 schema:givenName Ram K.
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01212430255.34
207 rdf:type schema:Person
208 sg:person.01242460441.07 schema:affiliation grid-institutes:grid.417631.6
209 schema:familyName Gupta
210 schema:givenName Madan M.
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242460441.07
212 rdf:type schema:Person
213 sg:person.0746716663.99 schema:affiliation grid-institutes:grid.417631.6
214 schema:familyName Shukla
215 schema:givenName Ashutosh K.
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0746716663.99
217 rdf:type schema:Person
218 sg:pub.10.1007/bf00043397 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050583998
219 https://doi.org/10.1007/bf00043397
220 rdf:type schema:CreativeWork
221 sg:pub.10.1007/s00425-004-1380-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037908902
222 https://doi.org/10.1007/s00425-004-1380-9
223 rdf:type schema:CreativeWork
224 sg:pub.10.1007/s10529-008-9881-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000020253
225 https://doi.org/10.1007/s10529-008-9881-4
226 rdf:type schema:CreativeWork
227 sg:pub.10.1023/a:1026526522555 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035254423
228 https://doi.org/10.1023/a:1026526522555
229 rdf:type schema:CreativeWork
230 sg:pub.10.1023/b:bile.0000023028.21985.07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028991840
231 https://doi.org/10.1023/b:bile.0000023028.21985.07
232 rdf:type schema:CreativeWork
233 sg:pub.10.1023/b:bile.0000025879.53632.f2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039737788
234 https://doi.org/10.1023/b:bile.0000025879.53632.f2
235 rdf:type schema:CreativeWork
236 sg:pub.10.1038/nchembio.160 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017938264
237 https://doi.org/10.1038/nchembio.160
238 rdf:type schema:CreativeWork
239 sg:pub.10.1385/mb:22:1:001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007314022
240 https://doi.org/10.1385/mb:22:1:001
241 rdf:type schema:CreativeWork
242 grid-institutes:None schema:alternateName C41-42, Double Storey, Ramesh Nagar, 110015, New Delhi, India
243 schema:name C41-42, Double Storey, Ramesh Nagar, 110015, New Delhi, India
244 Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India
245 rdf:type schema:Organization
246 grid-institutes:grid.417631.6 schema:alternateName Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India
247 Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India
248 Plant Tissue Culture Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India
249 schema:name Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India
250 Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India
251 Plant Tissue Culture Division, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, 226015, Lucknow, India
252 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...