Tandem repetition of baculovirus ie1 promoter results in upregulation of transcription View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-07

AUTHORS

K. Kojima, T. Hayakawa, S. Asano, H. Bando

ABSTRACT

In order to improve a virus-independent gene expression system in insect cells, the effect of the repetition of the promoter sequence of a baculovirus immediate early gene, BmNPV ie1, on the gene expression activity was examined. Tandem repetition of ie1 promoter resulted in about a 4-fold increase of the reporter gene expression activity. Primer extension analysis revealed that the repetition increased not only the amount of transcripts (4 to 5-times) but also the number of sites of transcription initiation (at least 3 extra initiation sites). A mutational experiment demonstrated the involvement of independent cis-acting elements in these two different promoter-enhancing effects. More... »

PAGES

1407-1414

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s007050170101

DOI

http://dx.doi.org/10.1007/s007050170101

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030633510

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11556716


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