Cloning and sequencing of columbid circovirus (CoCV), a new circovirus from pigeons View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-12

AUTHORS

A. Mankertz, K. Hattermann, B. Ehlers, D. Soike

ABSTRACT

The complete nucleotide sequence of columbid circovirus (CoCV) isolated from pigeons is described. CoCV was amplified using a consensus primer PCR approach directed against conserved sequences within the rep genes of vertebrate circoviruses. The genome of CoCV is circular and 2037 nt in size. It displays 55% homology to the genome of psittacine beak and feather disease virus and is more distantly related (< 40% homology) to porcine circovirus type 1 and 2. Two major open reading frames were identified, encoding the replicase and the putative capsid protein of CoCV. A region similar to the origin of replication of other circoviruses was found: it encompasses a stem-loop structure with the nonamer 5'-TAGTATTAC, conserved in circo-, nano- and geminiviruses. Phylogenetic analyses suggest classification of CoCV as member of the genus Circovirus of the virus family Circoviridae. More... »

PAGES

2469-2479

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s007050070002

DOI

http://dx.doi.org/10.1007/s007050070002

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050820367

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11205099


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