Development of polyclonal-antibody-coated immunomagnetic beads for separation and detection of koi herpesvirus in large-volume samples View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2020-02-14

AUTHORS

Min Zhang, Yingying Li, Hongli Jing, Na Wang, Shaoqiang Wu, Qing Wang, Xiangmei Lin

ABSTRACT

To separate and concentrate koi herpesvirus (KHV) from large-volume samples, a separation method based on immunomagnetic beads (IMBs) coated with polyclonal antibody directed against KHV was developed. After treatment with IMBs, viral DNA was extracted from samples and used as a template for quantitative PCR (qPCR). The results showed that the concentration of the template DNA extracted from the virus that had been separated using IMBs was 9.65-fold higher than that from virus not treated with IMBs. The detection limit of the IMBs/qPCR method was found to be at least 10 times lower than that of qPCR alone. More... »

PAGES

973-976

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-020-04557-y

DOI

http://dx.doi.org/10.1007/s00705-020-04557-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1124870320

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/32060793


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