Application of the SureSelect target enrichment system for next-generation sequencing to obtain the complete genome sequence of bovine leukemia virus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-11

AUTHORS

Tung Duy Dao, Vuong Nghia Bui, Tsutomu Omatsu, Yukie Katayama, Tetsuya Mizutani, Haruko Ogawa, Kunitoshi Imai

ABSTRACT

In this study, the SureSelect target enrichment system for Illumina Multiplexed Sequencing was applied to proviral DNA sequencing of bovine leukemia virus (BLV). The complete genomic DNA sequences of four Vietnamese BLV strains were successfully obtained with high read depth values and a genome coverage of 100% across all sequenced samples, in less than one week. This study provides the first complete Vietnamese BLV genome sequences. Their genetic variability and phylogenetic relationship were also analyzed and compared with those of 28 whole BLV genome sequences from different parts of the world. The results obtained provided new insights into the genetic diversity of the BLV tax gene, and further enabled us to identify nucleotide mutations in the gene that might not have been detected with the commercial detection kit that is currently available. More... »

PAGES

3155-3159

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-018-3957-9

DOI

http://dx.doi.org/10.1007/s00705-018-3957-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1105773663

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30039314


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