Characterization and application of monoclonal antibodies against channel catfish virus (CCV) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-05-25

AUTHORS

Hongli Jing, Min Zhang, Shaoqiang Wu, Xiangmei Lin, Longying Gao, Na Wang

ABSTRACT

Three monoclonal antibodies (MAbs) (27E4, 17H2, 8B6) against channel catfish virus (CCV) were developed by immunizing Balb/C mice. Using indirect ELISA, these MAbs reacted only with CCV and not with three other fish viruses or nine fish cell lines. During western blotting analysis, MAb 27E4 recognized 170 kDa and 47 kDa proteins, while MAb 17H2 and MAb 8B6 recognized 47 kDa and 56 kDa proteins, respectively. Furthermore, a sandwich ELISA was developed for detection of CCV. The detection limit of the test was 105 TCID50/mL. More... »

PAGES

2503-2506

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-018-3884-9

DOI

http://dx.doi.org/10.1007/s00705-018-3884-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1104238469

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29802548


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195 grid-institutes:grid.488141.0 schema:alternateName Shekou in Shenzhen Entry-exit Inspection and Quarantine Bureau, 518067, Shenzhen, China
196 schema:name Shekou in Shenzhen Entry-exit Inspection and Quarantine Bureau, 518067, Shenzhen, China
197 rdf:type schema:Organization
 




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