High frequency of Polio-like Enterovirus C strains with differential clustering of CVA-13 and EV-C99 subgenotypes in a cohort of Malawian ... View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2018-05-28

AUTHORS

Lieke Brouwer, Sabine M. G. van der Sanden, Job C. J. Calis, Andrea H. L. Bruning, Steven Wang, Joanne G. Wildenbeest, Sjoerd P. H. Rebers, Kamija S. Phiri, Brenda M. Westerhuis, Michaël Boele van Hensbroek, Dasja Pajkrt, Katja C. Wolthers

ABSTRACT

Enteroviruses (EVs) are among the most commonly detected viruses infecting humans worldwide. Although the prevalence of EVs is widely studied, the status of EV prevalence in sub-Saharan Africa remains largely unknown. The objective of our present study was therefore to increase our knowledge on EV circulation in sub-Saharan Africa. We obtained 749 fecal samples from a cross-sectional study conducted on Malawian children aged 6 to 60 months. We tested the samples for the presence of EVs using real time PCR, and typed the positive samples based on partial viral protein 1 (VP1) sequences. A large proportion of the samples was EV positive (89.9%). 12.9% of the typed samples belonged to EV species A (EV-A), 48.6% to species B (EV-B) and 38.5% to species C (EV-C). More than half of the EV-C strains (53%) belonged to subgroup C containing, among others, Poliovirus (PV) 1-3. The serotype most frequently isolated in our study was CVA-13, followed by EV-C99. The strains of CVA-13 showed a vast genetic diversity, possibly representing a new cluster, ‘F’. The majority of the EV-C99 strains grouped together as cluster B. In conclusion, this study showed a vast circulation of EVs among Malawian children, with an EV prevalence of 89.9%. Identification of prevalences for species EV-C comparable to our study (38.5%) have only previously been reported in sub-Saharan Africa, and EV-C is rarely found outside of this region. The data found in this study are an important contribution to our current knowledge of EV epidemiology within sub-Saharan Africa. More... »

PAGES

2645-2653

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-018-3878-7

DOI

http://dx.doi.org/10.1007/s00705-018-3878-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1104262612

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29808442


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27 schema:description Enteroviruses (EVs) are among the most commonly detected viruses infecting humans worldwide. Although the prevalence of EVs is widely studied, the status of EV prevalence in sub-Saharan Africa remains largely unknown. The objective of our present study was therefore to increase our knowledge on EV circulation in sub-Saharan Africa. We obtained 749 fecal samples from a cross-sectional study conducted on Malawian children aged 6 to 60 months. We tested the samples for the presence of EVs using real time PCR, and typed the positive samples based on partial viral protein 1 (VP1) sequences. A large proportion of the samples was EV positive (89.9%). 12.9% of the typed samples belonged to EV species A (EV-A), 48.6% to species B (EV-B) and 38.5% to species C (EV-C). More than half of the EV-C strains (53%) belonged to subgroup C containing, among others, Poliovirus (PV) 1-3. The serotype most frequently isolated in our study was CVA-13, followed by EV-C99. The strains of CVA-13 showed a vast genetic diversity, possibly representing a new cluster, ‘F’. The majority of the EV-C99 strains grouped together as cluster B. In conclusion, this study showed a vast circulation of EVs among Malawian children, with an EV prevalence of 89.9%. Identification of prevalences for species EV-C comparable to our study (38.5%) have only previously been reported in sub-Saharan Africa, and EV-C is rarely found outside of this region. The data found in this study are an important contribution to our current knowledge of EV epidemiology within sub-Saharan Africa.
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34 B.
35 EV circulation
36 EV prevalence
37 EV-C99
38 Malawian children
39 PCR
40 Saharan Africa
41 children
42 circulation
43 cluster B.
44 clustering
45 clusters
46 cohort
47 comparables
48 conclusion
49 containing
50 contribution
51 cross-sectional study
52 current knowledge
53 data
54 differential clustering
55 diversity
56 enterovirus C
57 enteroviruses
58 epidemiology
59 fecal samples
60 frequency
61 genetic diversity
62 half
63 high frequency
64 humans
65 identification
66 identification of prevalence
67 important contribution
68 knowledge
69 large proportion
70 majority
71 months
72 new clusters
73 objective
74 poliovirus 1
75 positive samples
76 presence
77 presence of enteroviruses
78 present study
79 prevalence
80 prevalence of enterovirus
81 proportion
82 real-time PCR
83 region
84 samples
85 sequence
86 serotypes
87 status
88 strains
89 study
90 subgenotypes
91 time PCR
92 vast genetic diversity
93 viral protein 1 (VP1) sequences
94 virus
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