Characterization of cross-clade monoclonal antibodies against H5N1 highly pathogenic avian influenza virus and their application to the antigenic analysis of ... View Full Text


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Article Info

DATE

2017-04-12

AUTHORS

Dulyatad Gronsang, Anh N. Bui, Dai Q. Trinh, Vuong N. Bui, Khong V. Nguyen, Minh X. Can, Tsutomu Omatsu, Tetsuya Mizutani, Makoto Nagai, Yukie Katayama, Rapeewan Thampaisarn, Haruko Ogawa, Kunitoshi Imai

ABSTRACT

H5N1 highly pathogenic avian influenza viruses (HPAIVs) are a threat to both animal and public health and require specific and rapid detection for prompt disease control. We produced three neutralizing anti-hemagglutinin (HA) monoclonal antibodies (mAbs) using two clades (2.2 and 2.5) of the H5N1 HPAIV isolated in Japan. Blocking immunofluorescence tests showed that each mAb recognized different epitopes; 3B5.1 and 3B5.2 mAbs against the clade 2.5 virus showed cross-clade reactivity to all 26 strains from clades 1, 2.2, 2.3.2.1, 2.3.2.1a, b, c and 2.3.4, suggesting that the epitope(s) recognized are conserved. Conversely, the 1G5 mAb against the clade 2.2 virus showed reactivity to only clades 1, 2.3.4 and 2.5 strains. An analysis of escape mutants, and some clades of the H5N1 viruses recognized by 3B5.1 and 3B5.2 mAbs, suggested that the mAbs bind to an epitope, including amino acid residues at position 162 in the HA1 protein (R162 and K162). Unexpectedly, however, when five Eurasian-origin H5 low-pathogenic AIV (LPAIV) strains with R162 were examined (EA-nonGsGD clade) as well as two American-origin strains (Am-nonGsGD clade), the mAb recognized only EA-nonGsGD clade strains. The R162 and K162 residues in the HA1 protein were highly conserved among 36 of the 43 H5N1 clades reported, including clades 2.3.2.1a and 2.3.2.1c that are currently circulating in Asia, Africa and Europe. The amino acid residues (158-PTIKRSYNNTNQE-170) in the HA1 protein are probably an epitope responsible for the cross-clade reactivity of the mAbs, considering the epitopes reported elsewhere. The 3B5.1 and 3B5.2 mAbs may be useful for the specific detection of H5N1 HPAIVs circulating in the field. More... »

PAGES

2257-2269

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-017-3350-0

DOI

http://dx.doi.org/10.1007/s00705-017-3350-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084746114

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28405766


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27 schema:description H5N1 highly pathogenic avian influenza viruses (HPAIVs) are a threat to both animal and public health and require specific and rapid detection for prompt disease control. We produced three neutralizing anti-hemagglutinin (HA) monoclonal antibodies (mAbs) using two clades (2.2 and 2.5) of the H5N1 HPAIV isolated in Japan. Blocking immunofluorescence tests showed that each mAb recognized different epitopes; 3B5.1 and 3B5.2 mAbs against the clade 2.5 virus showed cross-clade reactivity to all 26 strains from clades 1, 2.2, 2.3.2.1, 2.3.2.1a, b, c and 2.3.4, suggesting that the epitope(s) recognized are conserved. Conversely, the 1G5 mAb against the clade 2.2 virus showed reactivity to only clades 1, 2.3.4 and 2.5 strains. An analysis of escape mutants, and some clades of the H5N1 viruses recognized by 3B5.1 and 3B5.2 mAbs, suggested that the mAbs bind to an epitope, including amino acid residues at position 162 in the HA1 protein (R162 and K162). Unexpectedly, however, when five Eurasian-origin H5 low-pathogenic AIV (LPAIV) strains with R162 were examined (EA-nonGsGD clade) as well as two American-origin strains (Am-nonGsGD clade), the mAb recognized only EA-nonGsGD clade strains. The R162 and K162 residues in the HA1 protein were highly conserved among 36 of the 43 H5N1 clades reported, including clades 2.3.2.1a and 2.3.2.1c that are currently circulating in Asia, Africa and Europe. The amino acid residues (158-PTIKRSYNNTNQE-170) in the HA1 protein are probably an epitope responsible for the cross-clade reactivity of the mAbs, considering the epitopes reported elsewhere. The 3B5.1 and 3B5.2 mAbs may be useful for the specific detection of H5N1 HPAIVs circulating in the field.
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35 Africa
36 American-origin strains
37 Asia
38 Disease Control
39 EA-nonGsGD clade strains
40 Eurasian-origin H5
41 Europe
42 H5
43 H5 subtype viruses
44 H5N1 HPAIVs
45 H5N1 virus
46 HA1 protein
47 HPAIV
48 Japan
49 K162
50 R162
51 acid residues
52 amino acid residues
53 analysis
54 animals
55 anti-hemagglutinin monoclonal antibodies
56 antibodies
57 antigenic analysis
58 applications
59 avian influenza viruses
60 binds
61 characterization
62 clade
63 clade 1
64 clade 2.2 viruses
65 clade strains
66 control
67 cross-clade monoclonal antibodies
68 cross-clade reactivity
69 detection
70 different epitopes
71 diverse H5 subtype viruses
72 epitopes
73 escape mutants
74 field
75 health
76 immunofluorescence test
77 influenza virus
78 mAbs
79 mAbs bind
80 monoclonal antibodies
81 mutants
82 pathogenic AIV
83 pathogenic avian influenza virus
84 position 162
85 prompt disease control
86 protein
87 public health
88 rapid detection
89 reactivity
90 residues
91 specific detection
92 strains
93 subtype viruses
94 test
95 threat
96 virus
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