Characterization of cross-clade monoclonal antibodies against H5N1 highly pathogenic avian influenza virus and their application to the antigenic analysis of ... View Full Text


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Article Info

DATE

2017-04-12

AUTHORS

Dulyatad Gronsang, Anh N. Bui, Dai Q. Trinh, Vuong N. Bui, Khong V. Nguyen, Minh X. Can, Tsutomu Omatsu, Tetsuya Mizutani, Makoto Nagai, Yukie Katayama, Rapeewan Thampaisarn, Haruko Ogawa, Kunitoshi Imai

ABSTRACT

H5N1 highly pathogenic avian influenza viruses (HPAIVs) are a threat to both animal and public health and require specific and rapid detection for prompt disease control. We produced three neutralizing anti-hemagglutinin (HA) monoclonal antibodies (mAbs) using two clades (2.2 and 2.5) of the H5N1 HPAIV isolated in Japan. Blocking immunofluorescence tests showed that each mAb recognized different epitopes; 3B5.1 and 3B5.2 mAbs against the clade 2.5 virus showed cross-clade reactivity to all 26 strains from clades 1, 2.2, 2.3.2.1, 2.3.2.1a, b, c and 2.3.4, suggesting that the epitope(s) recognized are conserved. Conversely, the 1G5 mAb against the clade 2.2 virus showed reactivity to only clades 1, 2.3.4 and 2.5 strains. An analysis of escape mutants, and some clades of the H5N1 viruses recognized by 3B5.1 and 3B5.2 mAbs, suggested that the mAbs bind to an epitope, including amino acid residues at position 162 in the HA1 protein (R162 and K162). Unexpectedly, however, when five Eurasian-origin H5 low-pathogenic AIV (LPAIV) strains with R162 were examined (EA-nonGsGD clade) as well as two American-origin strains (Am-nonGsGD clade), the mAb recognized only EA-nonGsGD clade strains. The R162 and K162 residues in the HA1 protein were highly conserved among 36 of the 43 H5N1 clades reported, including clades 2.3.2.1a and 2.3.2.1c that are currently circulating in Asia, Africa and Europe. The amino acid residues (158-PTIKRSYNNTNQE-170) in the HA1 protein are probably an epitope responsible for the cross-clade reactivity of the mAbs, considering the epitopes reported elsewhere. The 3B5.1 and 3B5.2 mAbs may be useful for the specific detection of H5N1 HPAIVs circulating in the field. More... »

PAGES

2257-2269

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-017-3350-0

DOI

http://dx.doi.org/10.1007/s00705-017-3350-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084746114

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28405766


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27 schema:description H5N1 highly pathogenic avian influenza viruses (HPAIVs) are a threat to both animal and public health and require specific and rapid detection for prompt disease control. We produced three neutralizing anti-hemagglutinin (HA) monoclonal antibodies (mAbs) using two clades (2.2 and 2.5) of the H5N1 HPAIV isolated in Japan. Blocking immunofluorescence tests showed that each mAb recognized different epitopes; 3B5.1 and 3B5.2 mAbs against the clade 2.5 virus showed cross-clade reactivity to all 26 strains from clades 1, 2.2, 2.3.2.1, 2.3.2.1a, b, c and 2.3.4, suggesting that the epitope(s) recognized are conserved. Conversely, the 1G5 mAb against the clade 2.2 virus showed reactivity to only clades 1, 2.3.4 and 2.5 strains. An analysis of escape mutants, and some clades of the H5N1 viruses recognized by 3B5.1 and 3B5.2 mAbs, suggested that the mAbs bind to an epitope, including amino acid residues at position 162 in the HA1 protein (R162 and K162). Unexpectedly, however, when five Eurasian-origin H5 low-pathogenic AIV (LPAIV) strains with R162 were examined (EA-nonGsGD clade) as well as two American-origin strains (Am-nonGsGD clade), the mAb recognized only EA-nonGsGD clade strains. The R162 and K162 residues in the HA1 protein were highly conserved among 36 of the 43 H5N1 clades reported, including clades 2.3.2.1a and 2.3.2.1c that are currently circulating in Asia, Africa and Europe. The amino acid residues (158-PTIKRSYNNTNQE-170) in the HA1 protein are probably an epitope responsible for the cross-clade reactivity of the mAbs, considering the epitopes reported elsewhere. The 3B5.1 and 3B5.2 mAbs may be useful for the specific detection of H5N1 HPAIVs circulating in the field.
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34 Africa
35 Asia
36 Europe
37 H5
38 H5 subtype viruses
39 H5N1 HPAIVs
40 H5N1 virus
41 HA1 protein
42 HPAIV
43 Japan
44 K162
45 R162
46 acid residues
47 amino acid residues
48 analysis
49 animals
50 anti-hemagglutinin monoclonal antibodies
51 antibodies
52 antigenic analysis
53 applications
54 avian influenza viruses
55 binds
56 characterization
57 clade
58 clade 1
59 clade 2.2 viruses
60 clade strains
61 control
62 cross-clade reactivity
63 detection
64 different epitopes
65 disease control
66 epitopes
67 escape mutants
68 field
69 health
70 immunofluorescence test
71 influenza virus
72 mAbs
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74 monoclonal antibodies
75 mutants
76 pathogenic AIV
77 pathogenic avian influenza viruses
78 position 162
79 protein
80 public health
81 rapid detection
82 reactivity
83 residues
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85 strains
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