Experimental infection of gnotobiotic pigs with the cell-culture-adapted porcine deltacoronavirus strain OH-FD22 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2016-12

AUTHORS

Hui Hu, Kwonil Jung, Anastasia N. Vlasova, Linda J. Saif

ABSTRACT

Porcine deltacoronavirus (PDCoV) is a novel enteropathogenic coronavirus in pigs. We have isolated and passaged the PDCoV strain OH-FD22 in an LLC porcine kidney (LLC-PK) cell line. Our study investigated the pathogenicity of the tissue-culture-grown PDCoV (TC-PDCoV) OH-FD22 at cell passages 5, 20 and 40 in LLC-PK cells, in eight 14-day-old gnotobiotic (Gn) pigs. Pigs (n = 3) were euthanized for pathologic examination at post-inoculation day (PID) 3, and the remainder were monitored for clinical signs, virus shedding, and serum antibody responses until PID 28. All inoculated pigs developed watery diarrhea and/or vomiting at PID 1-2 and shed the highest amount of viral RNA in feces at PID 3-5, accompanied by severe atrophic enteritis. They developed high titers of PDCoV-specific IgG/IgA and virus-neutralizing antibodies in serum at PID 23-24. Histologic lesions were limited to the villous epithelium of the jejunum and ileum at PID 3. Two inoculated pigs tested at PID 23-24 had small to moderate numbers of PDCoV antigen-positive cells in the intestinal lamina propria and mesenteric lymph nodes, but not in enterocytes. An analysis of full-length S and N genes of TC- and Gn-pig-passaged OH-FD22 revealed a high genetic stability in cell culture and pigs. TC-PDCoV OH-FD22 (cell passages 5, 20 and 40) was enteropathogenic, and the pathogenicity was similar to that of the original field virus. The TC-PDCoV OH-FD22 will be useful for further pathogenesis studies and for evaluating if higher-level cell-culture passaged virus becomes attenuated for vaccine development. More... »

PAGES

3421-3434

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-016-3056-8

DOI

http://dx.doi.org/10.1007/s00705-016-3056-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016027129

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27619798


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adaptation, Biological", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animal Experimentation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Neutralizing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Coronavirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Coronavirus Infections", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Diarrhea", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Feces", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genomic Instability", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Germ-Free Life", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Histocytochemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunoglobulin A", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunoglobulin G", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Intestinal Mucosa", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Jejunum", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Serial Passage", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Swine", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Swine Diseases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viral Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virus Shedding", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Henan Agricultural University", 
          "id": "https://www.grid.ac/institutes/grid.108266.b", 
          "name": [
            "Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Ave., 44691, Wooster, OH, USA", 
            "College of Animal Science and Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hu", 
        "givenName": "Hui", 
        "id": "sg:person.0666062176.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0666062176.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Ave., 44691, Wooster, OH, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jung", 
        "givenName": "Kwonil", 
        "id": "sg:person.01345655335.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345655335.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Ave., 44691, Wooster, OH, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Vlasova", 
        "givenName": "Anastasia N.", 
        "id": "sg:person.01277542135.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277542135.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Ave., 44691, Wooster, OH, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saif", 
        "givenName": "Linda J.", 
        "id": "sg:person.011005227177.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011005227177.12"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.vetmic.2015.06.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003108276"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.00031-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005469959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetmic.2014.08.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006380292"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00705-013-1942-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008597467", 
          "https://doi.org/10.1007/s00705-013-1942-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid2204.151852", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009853103"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid2104.141859", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012069781"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/tbed.12399", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020896539"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/genomea.01191-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021845597"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetmic.2013.01.039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023079900"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid2008.140526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023305808"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid2112.150283", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023648400"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tvjl.2005.02.016", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024158121"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.06540-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026613775"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid2007.140296", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031797557"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0165-2427(01)00417-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032020194"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/genomea.00278-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036869147"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetmic.2014.09.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037268811"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vetmic.2013.11.008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037955683"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid2004.131685", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038219507"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.vaccine.2006.12.032", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039487339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/104063870001200418", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039641187"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/104063870001200418", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039641187"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3390/v2081803", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040683156"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.virusres.2016.04.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043412630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/genomea.00218-14", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047281949"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.virol.2015.03.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048627445"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0124363", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049272035"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mbio.00064-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062727364"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2016-12", 
    "datePublishedReg": "2016-12-01", 
    "description": "Porcine deltacoronavirus (PDCoV) is a novel enteropathogenic coronavirus in pigs. We have isolated and passaged the PDCoV strain OH-FD22 in an LLC porcine kidney (LLC-PK) cell line. Our study investigated the pathogenicity of the tissue-culture-grown PDCoV (TC-PDCoV) OH-FD22 at cell passages 5, 20 and 40 in LLC-PK cells, in eight 14-day-old gnotobiotic (Gn) pigs. Pigs (n\u00a0=\u00a03) were euthanized for pathologic examination at post-inoculation day (PID) 3, and the remainder were monitored for clinical signs, virus shedding, and serum antibody responses until PID 28. All inoculated pigs developed watery diarrhea and/or vomiting at PID 1-2 and shed the highest amount of viral RNA in feces at PID 3-5, accompanied by severe atrophic enteritis. They developed high titers of PDCoV-specific IgG/IgA and virus-neutralizing antibodies in serum at PID 23-24. Histologic lesions were limited to the villous epithelium of the jejunum and ileum at PID 3. Two inoculated pigs tested at PID 23-24 had small to moderate numbers of PDCoV antigen-positive cells in the intestinal lamina propria and mesenteric lymph nodes, but not in enterocytes. An analysis of full-length S and N genes of TC- and Gn-pig-passaged OH-FD22 revealed a high\u00a0genetic stability in cell culture and pigs. TC-PDCoV OH-FD22 (cell passages 5, 20 and 40) was enteropathogenic, and the pathogenicity was similar to that of the original field virus. The TC-PDCoV OH-FD22 will be useful for further pathogenesis studies and for evaluating if higher-level cell-culture passaged virus becomes attenuated for vaccine development.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00705-016-3056-8", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083335", 
        "issn": [
          "0304-8608", 
          "1432-8798"
        ], 
        "name": "Archives of Virology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "12", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "161"
      }
    ], 
    "name": "Experimental infection of gnotobiotic pigs with the cell-culture-adapted porcine deltacoronavirus strain OH-FD22", 
    "pagination": "3421-3434", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "4c5dc42ce1fbf0d29025865047c88034fab65ba3820bed395c7b4bc0e6d7e880"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "27619798"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "7506870"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00705-016-3056-8"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1016027129"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00705-016-3056-8", 
      "https://app.dimensions.ai/details/publication/pub.1016027129"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:25", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000362_0000000362/records_87106_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs00705-016-3056-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00705-016-3056-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00705-016-3056-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00705-016-3056-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00705-016-3056-8'


 

This table displays all metadata directly associated to this object as RDF triples.

271 TRIPLES      21 PREDICATES      80 URIs      44 LITERALS      32 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00705-016-3056-8 schema:about N1fcc523fa3914f2a83b5aba89b0df9ac
2 N241acf02001e40a1bc02f407a2661d57
3 N41c25f24851e42b988a8492dd5604633
4 N41fed920a67b4ff7b7abfd5ec297c169
5 N421e1d889fc84424a1626c7edc617f62
6 N5d089bb0f4924314a9f726470f3d290b
7 N73c2493f2ce54ad8ba7f7a7935b62977
8 N78d3922abf684e5dadcd869361577873
9 N79ed4758690946789c2e09eb0ea3f940
10 N7a9e504eec664111856e1a3377ef66e4
11 N8df6bdb133ab4ea89ad50f427dc1e971
12 N9f744eef2a944139b307306fb04da1e9
13 Naeb74de967314693809259d1b4894070
14 Nb6566b78db644e138d95764267c3537d
15 Nb89a5e9b49f543048777467f74f9b8df
16 Nb983b1ce93234b6a8a1177f385418c70
17 Nc95e415265af498a955bdefcad720dac
18 Ncba4690f100a4d32afd4ed1c99f28017
19 Nd00440c2b5624924a5f143dd4e05d5ef
20 Nd1482b896cbb4eadbbec577236956db0
21 Ne284c4dbe7cb49d28457510d982ad7f4
22 Ne7d60e3f8cb747deae727f32462b603e
23 Nee5ae29a85c84f47a69d33c4e060407f
24 anzsrc-for:11
25 anzsrc-for:1107
26 schema:author Nc437d9c5e5554a14845ae37007065d4b
27 schema:citation sg:pub.10.1007/s00705-013-1942-x
28 https://doi.org/10.1016/0003-2697(76)90527-3
29 https://doi.org/10.1016/j.tvjl.2005.02.016
30 https://doi.org/10.1016/j.vaccine.2006.12.032
31 https://doi.org/10.1016/j.vetmic.2013.01.039
32 https://doi.org/10.1016/j.vetmic.2013.11.008
33 https://doi.org/10.1016/j.vetmic.2014.08.012
34 https://doi.org/10.1016/j.vetmic.2014.09.002
35 https://doi.org/10.1016/j.vetmic.2015.06.005
36 https://doi.org/10.1016/j.virol.2015.03.024
37 https://doi.org/10.1016/j.virusres.2016.04.009
38 https://doi.org/10.1016/s0165-2427(01)00417-2
39 https://doi.org/10.1111/tbed.12399
40 https://doi.org/10.1128/genomea.00218-14
41 https://doi.org/10.1128/genomea.00278-14
42 https://doi.org/10.1128/genomea.01191-14
43 https://doi.org/10.1128/jcm.00031-15
44 https://doi.org/10.1128/jvi.06540-11
45 https://doi.org/10.1128/mbio.00064-15
46 https://doi.org/10.1177/104063870001200418
47 https://doi.org/10.1371/journal.pone.0124363
48 https://doi.org/10.3201/eid2004.131685
49 https://doi.org/10.3201/eid2007.140296
50 https://doi.org/10.3201/eid2008.140526
51 https://doi.org/10.3201/eid2104.141859
52 https://doi.org/10.3201/eid2112.150283
53 https://doi.org/10.3201/eid2204.151852
54 https://doi.org/10.3390/v2081803
55 schema:datePublished 2016-12
56 schema:datePublishedReg 2016-12-01
57 schema:description Porcine deltacoronavirus (PDCoV) is a novel enteropathogenic coronavirus in pigs. We have isolated and passaged the PDCoV strain OH-FD22 in an LLC porcine kidney (LLC-PK) cell line. Our study investigated the pathogenicity of the tissue-culture-grown PDCoV (TC-PDCoV) OH-FD22 at cell passages 5, 20 and 40 in LLC-PK cells, in eight 14-day-old gnotobiotic (Gn) pigs. Pigs (n = 3) were euthanized for pathologic examination at post-inoculation day (PID) 3, and the remainder were monitored for clinical signs, virus shedding, and serum antibody responses until PID 28. All inoculated pigs developed watery diarrhea and/or vomiting at PID 1-2 and shed the highest amount of viral RNA in feces at PID 3-5, accompanied by severe atrophic enteritis. They developed high titers of PDCoV-specific IgG/IgA and virus-neutralizing antibodies in serum at PID 23-24. Histologic lesions were limited to the villous epithelium of the jejunum and ileum at PID 3. Two inoculated pigs tested at PID 23-24 had small to moderate numbers of PDCoV antigen-positive cells in the intestinal lamina propria and mesenteric lymph nodes, but not in enterocytes. An analysis of full-length S and N genes of TC- and Gn-pig-passaged OH-FD22 revealed a high genetic stability in cell culture and pigs. TC-PDCoV OH-FD22 (cell passages 5, 20 and 40) was enteropathogenic, and the pathogenicity was similar to that of the original field virus. The TC-PDCoV OH-FD22 will be useful for further pathogenesis studies and for evaluating if higher-level cell-culture passaged virus becomes attenuated for vaccine development.
58 schema:genre research_article
59 schema:inLanguage en
60 schema:isAccessibleForFree false
61 schema:isPartOf N48a15ec432c143a8b582ebfaaa7e0c23
62 Nb36c47fe286747b08c677cc808762a3c
63 sg:journal.1083335
64 schema:name Experimental infection of gnotobiotic pigs with the cell-culture-adapted porcine deltacoronavirus strain OH-FD22
65 schema:pagination 3421-3434
66 schema:productId N0da41890ccfd4484950491d98d1a3a13
67 N41dbc730bf1f4d1096e3664cfc9e557d
68 N4433abef2cf044ed92cfbcd60df44764
69 Na0f54b0b69ae44b99119a9f9a650549f
70 Nf9c67f4ad3dc4e5aa9ee9961ceb85b73
71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016027129
72 https://doi.org/10.1007/s00705-016-3056-8
73 schema:sdDatePublished 2019-04-11T12:25
74 schema:sdLicense https://scigraph.springernature.com/explorer/license/
75 schema:sdPublisher Ne3e433b3161a46499abdae719a2a3b99
76 schema:url https://link.springer.com/10.1007%2Fs00705-016-3056-8
77 sgo:license sg:explorer/license/
78 sgo:sdDataset articles
79 rdf:type schema:ScholarlyArticle
80 N0da41890ccfd4484950491d98d1a3a13 schema:name nlm_unique_id
81 schema:value 7506870
82 rdf:type schema:PropertyValue
83 N1fcc523fa3914f2a83b5aba89b0df9ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Swine Diseases
85 rdf:type schema:DefinedTerm
86 N241acf02001e40a1bc02f407a2661d57 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Coronavirus
88 rdf:type schema:DefinedTerm
89 N41c25f24851e42b988a8492dd5604633 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Viral Proteins
91 rdf:type schema:DefinedTerm
92 N41dbc730bf1f4d1096e3664cfc9e557d schema:name readcube_id
93 schema:value 4c5dc42ce1fbf0d29025865047c88034fab65ba3820bed395c7b4bc0e6d7e880
94 rdf:type schema:PropertyValue
95 N41fed920a67b4ff7b7abfd5ec297c169 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Histocytochemistry
97 rdf:type schema:DefinedTerm
98 N421e1d889fc84424a1626c7edc617f62 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Antibodies, Viral
100 rdf:type schema:DefinedTerm
101 N4433abef2cf044ed92cfbcd60df44764 schema:name pubmed_id
102 schema:value 27619798
103 rdf:type schema:PropertyValue
104 N48a15ec432c143a8b582ebfaaa7e0c23 schema:issueNumber 12
105 rdf:type schema:PublicationIssue
106 N5d089bb0f4924314a9f726470f3d290b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Cell Line
108 rdf:type schema:DefinedTerm
109 N632c608c108e453eb811e629ed555bec rdf:first sg:person.01277542135.20
110 rdf:rest Ncf1d4668aeca48d28179d19ae3184fc6
111 N73c2493f2ce54ad8ba7f7a7935b62977 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Jejunum
113 rdf:type schema:DefinedTerm
114 N78d3922abf684e5dadcd869361577873 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Immunoglobulin G
116 rdf:type schema:DefinedTerm
117 N79ed4758690946789c2e09eb0ea3f940 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Genomic Instability
119 rdf:type schema:DefinedTerm
120 N7a9e504eec664111856e1a3377ef66e4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Swine
122 rdf:type schema:DefinedTerm
123 N8df6bdb133ab4ea89ad50f427dc1e971 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Diarrhea
125 rdf:type schema:DefinedTerm
126 N9f744eef2a944139b307306fb04da1e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Adaptation, Biological
128 rdf:type schema:DefinedTerm
129 Na0f54b0b69ae44b99119a9f9a650549f schema:name dimensions_id
130 schema:value pub.1016027129
131 rdf:type schema:PropertyValue
132 Naa074fd1f95648d8876c0b50d1bf1968 rdf:first sg:person.01345655335.04
133 rdf:rest N632c608c108e453eb811e629ed555bec
134 Naeb74de967314693809259d1b4894070 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Germ-Free Life
136 rdf:type schema:DefinedTerm
137 Nb36c47fe286747b08c677cc808762a3c schema:volumeNumber 161
138 rdf:type schema:PublicationVolume
139 Nb6566b78db644e138d95764267c3537d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Intestinal Mucosa
141 rdf:type schema:DefinedTerm
142 Nb89a5e9b49f543048777467f74f9b8df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Serial Passage
144 rdf:type schema:DefinedTerm
145 Nb983b1ce93234b6a8a1177f385418c70 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Sequence Analysis, DNA
147 rdf:type schema:DefinedTerm
148 Nc437d9c5e5554a14845ae37007065d4b rdf:first sg:person.0666062176.70
149 rdf:rest Naa074fd1f95648d8876c0b50d1bf1968
150 Nc95e415265af498a955bdefcad720dac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Animal Experimentation
152 rdf:type schema:DefinedTerm
153 Ncba4690f100a4d32afd4ed1c99f28017 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Antibodies, Neutralizing
155 rdf:type schema:DefinedTerm
156 Ncf1d4668aeca48d28179d19ae3184fc6 rdf:first sg:person.011005227177.12
157 rdf:rest rdf:nil
158 Nd00440c2b5624924a5f143dd4e05d5ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Animals
160 rdf:type schema:DefinedTerm
161 Nd1482b896cbb4eadbbec577236956db0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Feces
163 rdf:type schema:DefinedTerm
164 Ne284c4dbe7cb49d28457510d982ad7f4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
165 schema:name Virus Shedding
166 rdf:type schema:DefinedTerm
167 Ne3e433b3161a46499abdae719a2a3b99 schema:name Springer Nature - SN SciGraph project
168 rdf:type schema:Organization
169 Ne7d60e3f8cb747deae727f32462b603e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
170 schema:name Coronavirus Infections
171 rdf:type schema:DefinedTerm
172 Nee5ae29a85c84f47a69d33c4e060407f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name Immunoglobulin A
174 rdf:type schema:DefinedTerm
175 Nf9c67f4ad3dc4e5aa9ee9961ceb85b73 schema:name doi
176 schema:value 10.1007/s00705-016-3056-8
177 rdf:type schema:PropertyValue
178 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
179 schema:name Medical and Health Sciences
180 rdf:type schema:DefinedTerm
181 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
182 schema:name Immunology
183 rdf:type schema:DefinedTerm
184 sg:journal.1083335 schema:issn 0304-8608
185 1432-8798
186 schema:name Archives of Virology
187 rdf:type schema:Periodical
188 sg:person.011005227177.12 schema:affiliation https://www.grid.ac/institutes/grid.261331.4
189 schema:familyName Saif
190 schema:givenName Linda J.
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011005227177.12
192 rdf:type schema:Person
193 sg:person.01277542135.20 schema:affiliation https://www.grid.ac/institutes/grid.261331.4
194 schema:familyName Vlasova
195 schema:givenName Anastasia N.
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01277542135.20
197 rdf:type schema:Person
198 sg:person.01345655335.04 schema:affiliation https://www.grid.ac/institutes/grid.261331.4
199 schema:familyName Jung
200 schema:givenName Kwonil
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345655335.04
202 rdf:type schema:Person
203 sg:person.0666062176.70 schema:affiliation https://www.grid.ac/institutes/grid.108266.b
204 schema:familyName Hu
205 schema:givenName Hui
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0666062176.70
207 rdf:type schema:Person
208 sg:pub.10.1007/s00705-013-1942-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1008597467
209 https://doi.org/10.1007/s00705-013-1942-x
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/j.tvjl.2005.02.016 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024158121
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/j.vaccine.2006.12.032 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039487339
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/j.vetmic.2013.01.039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023079900
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/j.vetmic.2013.11.008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037955683
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/j.vetmic.2014.08.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006380292
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/j.vetmic.2014.09.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037268811
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/j.vetmic.2015.06.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003108276
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/j.virol.2015.03.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048627445
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/j.virusres.2016.04.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043412630
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1016/s0165-2427(01)00417-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032020194
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1111/tbed.12399 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020896539
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1128/genomea.00218-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047281949
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1128/genomea.00278-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036869147
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1128/genomea.01191-14 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021845597
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1128/jcm.00031-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005469959
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1128/jvi.06540-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026613775
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1128/mbio.00064-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062727364
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1177/104063870001200418 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039641187
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1371/journal.pone.0124363 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049272035
250 rdf:type schema:CreativeWork
251 https://doi.org/10.3201/eid2004.131685 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038219507
252 rdf:type schema:CreativeWork
253 https://doi.org/10.3201/eid2007.140296 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031797557
254 rdf:type schema:CreativeWork
255 https://doi.org/10.3201/eid2008.140526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023305808
256 rdf:type schema:CreativeWork
257 https://doi.org/10.3201/eid2104.141859 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012069781
258 rdf:type schema:CreativeWork
259 https://doi.org/10.3201/eid2112.150283 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023648400
260 rdf:type schema:CreativeWork
261 https://doi.org/10.3201/eid2204.151852 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009853103
262 rdf:type schema:CreativeWork
263 https://doi.org/10.3390/v2081803 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040683156
264 rdf:type schema:CreativeWork
265 https://www.grid.ac/institutes/grid.108266.b schema:alternateName Henan Agricultural University
266 schema:name College of Animal Science and Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China
267 Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Ave., 44691, Wooster, OH, USA
268 rdf:type schema:Organization
269 https://www.grid.ac/institutes/grid.261331.4 schema:alternateName The Ohio State University
270 schema:name Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, 1680 Madison Ave., 44691, Wooster, OH, USA
271 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...