Failure of propagation of human norovirus in intestinal epithelial cells with microvilli grown in three-dimensional cultures View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2014-02

AUTHORS

Sayaka Takanashi, Linda J. Saif, John H. Hughes, Tea Meulia, Kwonil Jung, Kelly A. Scheuer, Qiuhong Wang

ABSTRACT

Human noroviruses (HuNoVs) are a leading cause of acute gastroenteritis. Establishment of a cell culture system for in vitro HuNoV growth remains challenging. Replication of HuNoVs in human intestinal cell lines (INT-407 and Caco-2) that differentiate to produce microvilli in rotation wall vessel (RWV) three-dimensional cultures has been reported (Straub et al. in Emerg Infect Dis 13:396-403, 2007; J Water Health 9:225-240, 2011, and Water Sci Technol 67:863-868, 2013). We used a similar RWV system, intestinal cell lines, and the same (Genogroup [G] I.1) plus additional (GII.4 and GII.12) HuNoV strains to test the system's reproducibility and to expand the earlier findings. Apical microvilli were observed on the surface of both cell lines by light and electron microscopy. However, none of the cell types tested resulted in productive viral replication of any of the HuNoV strains, as confirmed by plateau or declining viral RNA titers in the supernatants and cell lysates of HuNoV-infected cells, determined by real-time reverse transcription PCR. These trends were the same when culture supplements were added that have been reported to be effective for replication of other fastidious enteric viruses in vitro. Additionally, by confocal microscopy and orthoslice analysis, viral capsid proteins were mainly observed above the actin filament signals, which suggested that the majority of viral antigens were on the cell surface. We conclude that even intestinal cells displaying microvilli were not sufficient to support HuNoV replication under the conditions tested. More... »

PAGES

257-266

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-013-1806-4

DOI

http://dx.doi.org/10.1007/s00705-013-1806-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041495798

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23974469


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epithelial Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microvilli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Norovirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viral Load", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virus Replication", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Tokyo", 
          "id": "https://www.grid.ac/institutes/grid.26999.3d", 
          "name": [
            "Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, 44691, Wooster, OH, USA", 
            "Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, 1900 Coffey Road, Columbus, OH, USA", 
            "Department of Developmental Medical Sciences, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Takanashi", 
        "givenName": "Sayaka", 
        "id": "sg:person.0657342343.94", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657342343.94"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, 44691, Wooster, OH, USA", 
            "Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, 1900 Coffey Road, Columbus, OH, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saif", 
        "givenName": "Linda J.", 
        "id": "sg:person.011005227177.12", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011005227177.12"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Department of Molecular Virology, Immunology and Medical Genetics, College of Medicine, The Ohio State University, 700 Children\u2019s Drive, Columbus, OH, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hughes", 
        "givenName": "John H.", 
        "id": "sg:person.0652052175.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652052175.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Molecular and Cellular Imaging Center, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Meulia", 
        "givenName": "Tea", 
        "id": "sg:person.0604263433.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604263433.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, 44691, Wooster, OH, USA", 
            "Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, 1900 Coffey Road, Columbus, OH, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jung", 
        "givenName": "Kwonil", 
        "id": "sg:person.01345655335.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345655335.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, 44691, Wooster, OH, USA", 
            "Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, 1900 Coffey Road, Columbus, OH, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Scheuer", 
        "givenName": "Kelly A.", 
        "id": "sg:person.0627763753.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627763753.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "The Ohio State University", 
          "id": "https://www.grid.ac/institutes/grid.261331.4", 
          "name": [
            "Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, 44691, Wooster, OH, USA", 
            "Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, 1900 Coffey Road, Columbus, OH, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Qiuhong", 
        "id": "sg:person.01351016714.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01351016714.20"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1186/1743-422x-6-103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000987678", 
          "https://doi.org/10.1186/1743-422x-6-103"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0041619", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003541161"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.274.30.21209", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005060139"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.virol.2005.11.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006088621"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1303.060549", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008475691"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.11150/kansenshogakuzasshi1970.78.699", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009588125"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmra0804575", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010719744"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1903.121029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010725396"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.78.6.3035-3045.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012316223"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2006.07.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014573758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0006561", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014731179"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0063485", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014731576"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01310954", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015018764", 
          "https://doi.org/10.1007/bf01310954"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01310954", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015018764", 
          "https://doi.org/10.1007/bf01310954"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.virol.2010.07.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015315678"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jviromet.2003.08.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016106165"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jviromet.2003.08.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016106165"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/339883", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017585783"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/iai.69.11.7106-7120.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018775916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.79.24.15351-15355.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019542733"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12560-010-9038-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021302331", 
          "https://doi.org/10.1007/s12560-010-9038-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12560-010-9038-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021302331", 
          "https://doi.org/10.1007/s12560-010-9038-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.41.4.1548-1557.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023969555"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.00305-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024918382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1410.080117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025236577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.00005-09", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027887816"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.00809-06", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029288083"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.micinf.2006.02.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032128400"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.76.23.12335-12343.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034742685"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1086/507335", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035331588"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(12)60560-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039607824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1307.070131", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040828190"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0401126101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041338030"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1408.071114", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042081856"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.02346-05", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042596281"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1701.p11101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044528851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1586/erv.12.78", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046526926"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.ppat.1000983", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046552402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.06600-11", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046805340"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mbio.00159-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049933084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm860", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049952025", 
          "https://doi.org/10.1038/nm860"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm860", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049952025", 
          "https://doi.org/10.1038/nm860"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02577533", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050239206", 
          "https://doi.org/10.1007/bf02577533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02577533", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050239206", 
          "https://doi.org/10.1007/bf02577533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.01489-07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053603563"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1017/s0950268810000993", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054011463"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/infdis/122.3.227", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059694215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/vir.0.19478-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060400048"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2166/wh.2010.106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069136909"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2166/wst.2012.636", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069145821"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.7326/0003-4819-79-1-18", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1073731200"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1076820327", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3201/eid1701.091101p2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078323135"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079414728", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083316445", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-02", 
    "datePublishedReg": "2014-02-01", 
    "description": "Human noroviruses (HuNoVs) are a leading cause of acute gastroenteritis. Establishment of a cell culture system for in vitro HuNoV growth remains challenging. Replication of HuNoVs in human intestinal cell lines (INT-407 and Caco-2) that differentiate to produce microvilli in rotation wall vessel (RWV) three-dimensional cultures has been reported (Straub et al. in Emerg Infect Dis 13:396-403, 2007; J Water Health 9:225-240, 2011, and Water Sci Technol 67:863-868, 2013). We used a similar RWV system, intestinal cell lines, and the same (Genogroup [G] I.1) plus additional (GII.4 and GII.12) HuNoV strains to test the system's reproducibility and to expand the earlier findings. Apical microvilli were observed on the surface of both cell lines by light and electron microscopy. However, none of the cell types tested resulted in productive viral replication of any of the HuNoV strains, as confirmed by plateau or declining viral RNA titers in the supernatants and cell lysates of HuNoV-infected cells, determined by real-time reverse transcription PCR. These trends were the same when culture supplements were added that have been reported to be effective for replication of other fastidious enteric viruses in vitro. Additionally, by confocal microscopy and orthoslice analysis, viral capsid proteins were mainly observed above the actin filament signals, which suggested that the majority of viral antigens were on the cell surface. We conclude that even intestinal cells displaying microvilli were not sufficient to support HuNoV replication under the conditions tested. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00705-013-1806-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2599890", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1083335", 
        "issn": [
          "0304-8608", 
          "1432-8798"
        ], 
        "name": "Archives of Virology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "159"
      }
    ], 
    "name": "Failure of propagation of human norovirus in intestinal epithelial cells with microvilli grown in three-dimensional cultures", 
    "pagination": "257-266", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "c5acadb190de6ec20ee1b5907fe2cf77cd562e63ff0e644a41762aa9ed5043b4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "23974469"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "7506870"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00705-013-1806-4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1041495798"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00705-013-1806-4", 
      "https://app.dimensions.ai/details/publication/pub.1041495798"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:52", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000515.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00705-013-1806-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00705-013-1806-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00705-013-1806-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00705-013-1806-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00705-013-1806-4'


 

This table displays all metadata directly associated to this object as RDF triples.

305 TRIPLES      21 PREDICATES      87 URIs      29 LITERALS      17 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00705-013-1806-4 schema:about N195b58c9d30143cb92da8e3cf19413c6
2 N4366bdafc455448b87a7aa99f8088160
3 N475aa9387e004981aacaaaa4628d3b20
4 Nb5483fb283d54f0abdeeabe0b4864cd8
5 Ncdf533c36f5b46aaa10ebdd2ab7b57a6
6 Nd2d438f8fec04ac7982de24938eb21b9
7 Nf32151de76164bfda80a3d71a0631797
8 Nf43b63ff0ee7475aa03322ca941a0282
9 anzsrc-for:11
10 anzsrc-for:1108
11 schema:author Ned6adcced21e4faa85e2ec7a6350457e
12 schema:citation sg:pub.10.1007/bf01310954
13 sg:pub.10.1007/bf02577533
14 sg:pub.10.1007/s12560-010-9038-1
15 sg:pub.10.1038/nm860
16 sg:pub.10.1186/1743-422x-6-103
17 https://app.dimensions.ai/details/publication/pub.1076820327
18 https://app.dimensions.ai/details/publication/pub.1079414728
19 https://app.dimensions.ai/details/publication/pub.1083316445
20 https://doi.org/10.1016/j.cell.2006.07.024
21 https://doi.org/10.1016/j.jviromet.2003.08.009
22 https://doi.org/10.1016/j.micinf.2006.02.020
23 https://doi.org/10.1016/j.virol.2005.11.015
24 https://doi.org/10.1016/j.virol.2010.07.001
25 https://doi.org/10.1016/s0140-6736(12)60560-1
26 https://doi.org/10.1017/s0950268810000993
27 https://doi.org/10.1056/nejmra0804575
28 https://doi.org/10.1073/pnas.0401126101
29 https://doi.org/10.1074/jbc.274.30.21209
30 https://doi.org/10.1086/339883
31 https://doi.org/10.1086/507335
32 https://doi.org/10.1093/infdis/122.3.227
33 https://doi.org/10.1099/vir.0.19478-0
34 https://doi.org/10.11150/kansenshogakuzasshi1970.78.699
35 https://doi.org/10.1128/aem.06600-11
36 https://doi.org/10.1128/iai.69.11.7106-7120.2001
37 https://doi.org/10.1128/jcm.00305-11
38 https://doi.org/10.1128/jcm.41.4.1548-1557.2003
39 https://doi.org/10.1128/jvi.00005-09
40 https://doi.org/10.1128/jvi.00809-06
41 https://doi.org/10.1128/jvi.01489-07
42 https://doi.org/10.1128/jvi.02346-05
43 https://doi.org/10.1128/jvi.76.23.12335-12343.2002
44 https://doi.org/10.1128/jvi.78.6.3035-3045.2004
45 https://doi.org/10.1128/jvi.79.24.15351-15355.2005
46 https://doi.org/10.1128/mbio.00159-12
47 https://doi.org/10.1371/journal.pone.0006561
48 https://doi.org/10.1371/journal.pone.0041619
49 https://doi.org/10.1371/journal.pone.0063485
50 https://doi.org/10.1371/journal.ppat.1000983
51 https://doi.org/10.1586/erv.12.78
52 https://doi.org/10.2166/wh.2010.106
53 https://doi.org/10.2166/wst.2012.636
54 https://doi.org/10.3201/eid1303.060549
55 https://doi.org/10.3201/eid1307.070131
56 https://doi.org/10.3201/eid1408.071114
57 https://doi.org/10.3201/eid1410.080117
58 https://doi.org/10.3201/eid1701.091101p2
59 https://doi.org/10.3201/eid1701.p11101
60 https://doi.org/10.3201/eid1903.121029
61 https://doi.org/10.7326/0003-4819-79-1-18
62 schema:datePublished 2014-02
63 schema:datePublishedReg 2014-02-01
64 schema:description Human noroviruses (HuNoVs) are a leading cause of acute gastroenteritis. Establishment of a cell culture system for in vitro HuNoV growth remains challenging. Replication of HuNoVs in human intestinal cell lines (INT-407 and Caco-2) that differentiate to produce microvilli in rotation wall vessel (RWV) three-dimensional cultures has been reported (Straub et al. in Emerg Infect Dis 13:396-403, 2007; J Water Health 9:225-240, 2011, and Water Sci Technol 67:863-868, 2013). We used a similar RWV system, intestinal cell lines, and the same (Genogroup [G] I.1) plus additional (GII.4 and GII.12) HuNoV strains to test the system's reproducibility and to expand the earlier findings. Apical microvilli were observed on the surface of both cell lines by light and electron microscopy. However, none of the cell types tested resulted in productive viral replication of any of the HuNoV strains, as confirmed by plateau or declining viral RNA titers in the supernatants and cell lysates of HuNoV-infected cells, determined by real-time reverse transcription PCR. These trends were the same when culture supplements were added that have been reported to be effective for replication of other fastidious enteric viruses in vitro. Additionally, by confocal microscopy and orthoslice analysis, viral capsid proteins were mainly observed above the actin filament signals, which suggested that the majority of viral antigens were on the cell surface. We conclude that even intestinal cells displaying microvilli were not sufficient to support HuNoV replication under the conditions tested.
65 schema:genre research_article
66 schema:inLanguage en
67 schema:isAccessibleForFree true
68 schema:isPartOf N82c80cca8ad04d1eaa9ef49c0bfe7f32
69 Nea3004ea204f4796a614c7e5f362e04c
70 sg:journal.1083335
71 schema:name Failure of propagation of human norovirus in intestinal epithelial cells with microvilli grown in three-dimensional cultures
72 schema:pagination 257-266
73 schema:productId N33c96ab5a9954e15b2c0fa97654eec81
74 N493b9b6f47894842b83c11c70c25248e
75 N76b7c7ac1dec409eb0158064929d9045
76 N77a69533d28243cbaad0020d68e802fa
77 Nad0a5eef735140feb3c198274737f7bd
78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041495798
79 https://doi.org/10.1007/s00705-013-1806-4
80 schema:sdDatePublished 2019-04-10T15:52
81 schema:sdLicense https://scigraph.springernature.com/explorer/license/
82 schema:sdPublisher Nc81334e4ac8d43aa9174e0b5a9bb7738
83 schema:url http://link.springer.com/10.1007%2Fs00705-013-1806-4
84 sgo:license sg:explorer/license/
85 sgo:sdDataset articles
86 rdf:type schema:ScholarlyArticle
87 N16bb89ed92e04de7b7d37e215622db27 rdf:first sg:person.011005227177.12
88 rdf:rest Nfac5fc9d012d43ddb626e090bd90b04c
89 N195b58c9d30143cb92da8e3cf19413c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Cell Line
91 rdf:type schema:DefinedTerm
92 N33c96ab5a9954e15b2c0fa97654eec81 schema:name nlm_unique_id
93 schema:value 7506870
94 rdf:type schema:PropertyValue
95 N4366bdafc455448b87a7aa99f8088160 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Virus Replication
97 rdf:type schema:DefinedTerm
98 N475aa9387e004981aacaaaa4628d3b20 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Microscopy
100 rdf:type schema:DefinedTerm
101 N493b9b6f47894842b83c11c70c25248e schema:name doi
102 schema:value 10.1007/s00705-013-1806-4
103 rdf:type schema:PropertyValue
104 N5fa7057eb8234e3fa047047cebb5c5fe rdf:first sg:person.0604263433.52
105 rdf:rest Nc60cd7fe5bdc48a09b2d2261b335c6bc
106 N76b7c7ac1dec409eb0158064929d9045 schema:name pubmed_id
107 schema:value 23974469
108 rdf:type schema:PropertyValue
109 N77a69533d28243cbaad0020d68e802fa schema:name dimensions_id
110 schema:value pub.1041495798
111 rdf:type schema:PropertyValue
112 N82c80cca8ad04d1eaa9ef49c0bfe7f32 schema:volumeNumber 159
113 rdf:type schema:PublicationVolume
114 Nad0a5eef735140feb3c198274737f7bd schema:name readcube_id
115 schema:value c5acadb190de6ec20ee1b5907fe2cf77cd562e63ff0e644a41762aa9ed5043b4
116 rdf:type schema:PropertyValue
117 Nb5483fb283d54f0abdeeabe0b4864cd8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Microvilli
119 rdf:type schema:DefinedTerm
120 Nc60cd7fe5bdc48a09b2d2261b335c6bc rdf:first sg:person.01345655335.04
121 rdf:rest Nd227efb863934d7098f764de44e0b84c
122 Nc81334e4ac8d43aa9174e0b5a9bb7738 schema:name Springer Nature - SN SciGraph project
123 rdf:type schema:Organization
124 Ncdf533c36f5b46aaa10ebdd2ab7b57a6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Norovirus
126 rdf:type schema:DefinedTerm
127 Nd227efb863934d7098f764de44e0b84c rdf:first sg:person.0627763753.00
128 rdf:rest Nffe98e64fbb74647b8c0458794c35a9f
129 Nd2d438f8fec04ac7982de24938eb21b9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Epithelial Cells
131 rdf:type schema:DefinedTerm
132 Nea3004ea204f4796a614c7e5f362e04c schema:issueNumber 2
133 rdf:type schema:PublicationIssue
134 Ned6adcced21e4faa85e2ec7a6350457e rdf:first sg:person.0657342343.94
135 rdf:rest N16bb89ed92e04de7b7d37e215622db27
136 Nf32151de76164bfda80a3d71a0631797 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Humans
138 rdf:type schema:DefinedTerm
139 Nf43b63ff0ee7475aa03322ca941a0282 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Viral Load
141 rdf:type schema:DefinedTerm
142 Nfac5fc9d012d43ddb626e090bd90b04c rdf:first sg:person.0652052175.33
143 rdf:rest N5fa7057eb8234e3fa047047cebb5c5fe
144 Nffe98e64fbb74647b8c0458794c35a9f rdf:first sg:person.01351016714.20
145 rdf:rest rdf:nil
146 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
147 schema:name Medical and Health Sciences
148 rdf:type schema:DefinedTerm
149 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
150 schema:name Medical Microbiology
151 rdf:type schema:DefinedTerm
152 sg:grant.2599890 http://pending.schema.org/fundedItem sg:pub.10.1007/s00705-013-1806-4
153 rdf:type schema:MonetaryGrant
154 sg:journal.1083335 schema:issn 0304-8608
155 1432-8798
156 schema:name Archives of Virology
157 rdf:type schema:Periodical
158 sg:person.011005227177.12 schema:affiliation https://www.grid.ac/institutes/grid.261331.4
159 schema:familyName Saif
160 schema:givenName Linda J.
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011005227177.12
162 rdf:type schema:Person
163 sg:person.01345655335.04 schema:affiliation https://www.grid.ac/institutes/grid.261331.4
164 schema:familyName Jung
165 schema:givenName Kwonil
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345655335.04
167 rdf:type schema:Person
168 sg:person.01351016714.20 schema:affiliation https://www.grid.ac/institutes/grid.261331.4
169 schema:familyName Wang
170 schema:givenName Qiuhong
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01351016714.20
172 rdf:type schema:Person
173 sg:person.0604263433.52 schema:affiliation https://www.grid.ac/institutes/grid.261331.4
174 schema:familyName Meulia
175 schema:givenName Tea
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604263433.52
177 rdf:type schema:Person
178 sg:person.0627763753.00 schema:affiliation https://www.grid.ac/institutes/grid.261331.4
179 schema:familyName Scheuer
180 schema:givenName Kelly A.
181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627763753.00
182 rdf:type schema:Person
183 sg:person.0652052175.33 schema:affiliation https://www.grid.ac/institutes/grid.261331.4
184 schema:familyName Hughes
185 schema:givenName John H.
186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652052175.33
187 rdf:type schema:Person
188 sg:person.0657342343.94 schema:affiliation https://www.grid.ac/institutes/grid.26999.3d
189 schema:familyName Takanashi
190 schema:givenName Sayaka
191 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657342343.94
192 rdf:type schema:Person
193 sg:pub.10.1007/bf01310954 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015018764
194 https://doi.org/10.1007/bf01310954
195 rdf:type schema:CreativeWork
196 sg:pub.10.1007/bf02577533 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050239206
197 https://doi.org/10.1007/bf02577533
198 rdf:type schema:CreativeWork
199 sg:pub.10.1007/s12560-010-9038-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021302331
200 https://doi.org/10.1007/s12560-010-9038-1
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/nm860 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049952025
203 https://doi.org/10.1038/nm860
204 rdf:type schema:CreativeWork
205 sg:pub.10.1186/1743-422x-6-103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000987678
206 https://doi.org/10.1186/1743-422x-6-103
207 rdf:type schema:CreativeWork
208 https://app.dimensions.ai/details/publication/pub.1076820327 schema:CreativeWork
209 https://app.dimensions.ai/details/publication/pub.1079414728 schema:CreativeWork
210 https://app.dimensions.ai/details/publication/pub.1083316445 schema:CreativeWork
211 https://doi.org/10.1016/j.cell.2006.07.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014573758
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/j.jviromet.2003.08.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016106165
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/j.micinf.2006.02.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032128400
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/j.virol.2005.11.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006088621
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1016/j.virol.2010.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015315678
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1016/s0140-6736(12)60560-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039607824
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1017/s0950268810000993 schema:sameAs https://app.dimensions.ai/details/publication/pub.1054011463
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1056/nejmra0804575 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010719744
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1073/pnas.0401126101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041338030
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1074/jbc.274.30.21209 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005060139
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1086/339883 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017585783
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1086/507335 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035331588
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1093/infdis/122.3.227 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059694215
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1099/vir.0.19478-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060400048
238 rdf:type schema:CreativeWork
239 https://doi.org/10.11150/kansenshogakuzasshi1970.78.699 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009588125
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1128/aem.06600-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046805340
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1128/iai.69.11.7106-7120.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018775916
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1128/jcm.00305-11 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024918382
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1128/jcm.41.4.1548-1557.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023969555
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1128/jvi.00005-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027887816
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1128/jvi.00809-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029288083
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1128/jvi.01489-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053603563
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1128/jvi.02346-05 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042596281
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1128/jvi.76.23.12335-12343.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034742685
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1128/jvi.78.6.3035-3045.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012316223
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1128/jvi.79.24.15351-15355.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019542733
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1128/mbio.00159-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049933084
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1371/journal.pone.0006561 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014731179
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1371/journal.pone.0041619 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003541161
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1371/journal.pone.0063485 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014731576
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1371/journal.ppat.1000983 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046552402
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1586/erv.12.78 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046526926
274 rdf:type schema:CreativeWork
275 https://doi.org/10.2166/wh.2010.106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069136909
276 rdf:type schema:CreativeWork
277 https://doi.org/10.2166/wst.2012.636 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069145821
278 rdf:type schema:CreativeWork
279 https://doi.org/10.3201/eid1303.060549 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008475691
280 rdf:type schema:CreativeWork
281 https://doi.org/10.3201/eid1307.070131 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040828190
282 rdf:type schema:CreativeWork
283 https://doi.org/10.3201/eid1408.071114 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042081856
284 rdf:type schema:CreativeWork
285 https://doi.org/10.3201/eid1410.080117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025236577
286 rdf:type schema:CreativeWork
287 https://doi.org/10.3201/eid1701.091101p2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078323135
288 rdf:type schema:CreativeWork
289 https://doi.org/10.3201/eid1701.p11101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044528851
290 rdf:type schema:CreativeWork
291 https://doi.org/10.3201/eid1903.121029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010725396
292 rdf:type schema:CreativeWork
293 https://doi.org/10.7326/0003-4819-79-1-18 schema:sameAs https://app.dimensions.ai/details/publication/pub.1073731200
294 rdf:type schema:CreativeWork
295 https://www.grid.ac/institutes/grid.261331.4 schema:alternateName The Ohio State University
296 schema:name Department of Molecular Virology, Immunology and Medical Genetics, College of Medicine, The Ohio State University, 700 Children’s Drive, Columbus, OH, USA
297 Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, 1900 Coffey Road, Columbus, OH, USA
298 Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, 44691, Wooster, OH, USA
299 Molecular and Cellular Imaging Center, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH, USA
300 rdf:type schema:Organization
301 https://www.grid.ac/institutes/grid.26999.3d schema:alternateName University of Tokyo
302 schema:name Department of Developmental Medical Sciences, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan
303 Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, 1900 Coffey Road, Columbus, OH, USA
304 Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, 44691, Wooster, OH, USA
305 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...