Development of a full-length infectious clone of sunflower chlorotic mottle virus (SuCMoV) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-02

AUTHORS

N. Bejerman, F. Giolitti, S. de Breuil, S. Lenardon

ABSTRACT

A full-length cDNA clone (p35SuCMoV) of the sunflower chlorotic mottle virus common strain (SuCMoV-C) genomic RNA was constructed. Three cDNA fragments covering the whole genome of SuCMoV-C were cloned between a cauliflower mosaic virus 35S promoter and a nopaline synthase terminator. Mechanical inoculation of sunflower and Nicotiana occidentalis seedlings with p35SuCMoV DNA led to systemic infection. Symptoms induced by p35SuCMoV were similar to those caused by the wild-type SuCMoV-C but appeared four days later. Infection was confirmed by a western blot test, electron microscopy, RT-PCR and inoculation of progeny virions to sunflower seedlings. This is the first report about the construction of a biologically active, full-length cDNA copy of the SuCMoV-C RNA genome. More... »

PAGES

485-490

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-012-1497-2

DOI

http://dx.doi.org/10.1007/s00705-012-1497-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1009636659

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23081677


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