Multiple polyadenylated RNA viruses detected in pooled cultivated and wild plant samples View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2012-02

AUTHORS

Stephen J. Wylie, Hao Luo, Hua Li, Michael G. K. Jones

ABSTRACT

RNA extracted from 120 leaf specimens from 17 plant species was pooled, and polyadenylated RNA species were sequenced together without barcoding in one lane using massively parallel sequencing technology. After analysis, complete or partial genome sequences representing 20 virus isolates of 16 polyadenylated RNA species were identified. In three cases, 2-3 distinct isolates of a virus species co-infected the same plant. Twelve of the viruses identified were described previously and belonged to the genera Potyvirus, Nepovirus, Allexivirus, and Carlavirus. Four were unknown and are proposed as members of the genera Potyvirus, Sadwavirus, and Trichovirus. Virus sequences were subsequently matched to original host plants using RT-PCR assays. More... »

PAGES

271-284

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00705-011-1166-x

    DOI

    http://dx.doi.org/10.1007/s00705-011-1166-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1015389738

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22075920


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