Characterisation and quantitation of mutant and wild-type genomes of Hardenbergia mosaic virus isolates co-infecting a wild plant of Hardenbergia comptoniana View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-07

AUTHORS

Stephen J. Wylie, Michael G. K. Jones

ABSTRACT

Deep sequencing of the polyadenylated transcriptome of a wild plant of Hardenbergia comptoniana was used to distinguish with high support complete genome sequences of two distinct isolates of Hardenbergia mosaic virus (HarMV) co-infecting it. Isolates shared 82.0% nucleotide (nt) and 86.7% amino acid (aa) identity. Isolate 57.1 (9,628 nt) had a deletion of 17 contiguous aa within the conserved core region of the coat protein (CP), while isolate 57.2 (9,675 nt) had a wild-type CP. Overall, HarMV genomes accounted for 10.69% of total transcripts sequenced, and the mutant virus genome was 23.1% more highly expressed than the wild-type genome. More... »

PAGES

1251-1255

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-011-0965-4

DOI

http://dx.doi.org/10.1007/s00705-011-0965-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039017637

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21424731


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