The complete genome sequence of a Passion fruit woodiness virus isolate from Australia determined using deep sequencing, and its relationship ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2011-03

AUTHORS

Stephen J. Wylie, Michael G. K. Jones

ABSTRACT

The complete genome sequence (9,858 nucleotides) of the Passion fruit woodiness virus isolate MU-2 was determined using Illumina sequencing. The large open reading frame (ORF) encodes a polyprotein containing 3,086 amino acids, with an AUG start codon and UAA stop codon. The polyprotein yielded 11 proteins (P1, HC-Pro, P3, PIPO, 6K1, CI, 6K2, NIa-VPg, NIa-Pro, NIb and CP). Putative cleavage sites between them were identified by sequence comparison to those of other known potyviruses. Accuracy of the genome sequence information was provided by 42-1691-fold sequence coverage, and viral RNA accounted for 7.38% of total polyadenylated RNA from the host plant. More... »

PAGES

479-482

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-010-0845-3

DOI

http://dx.doi.org/10.1007/s00705-010-0845-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1033611054

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/21076846


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