Modification of hepatitis C virus 1b RNA polymerase to make a highly active JFH1-type polymerase by mutation of the thumb ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-05

AUTHORS

Leiyun Weng, Jiamu Du, Jingling Zhou, Jianping Ding, Takaji Wakita, Michinori Kohara, Tetsuya Toyoda

ABSTRACT

Hepatitis C virus (HCV) JFH1 efficiently replicates and produces infectious virus particles in cultured cells. We compared polymerase activity between JFH1 and 1b strains in vitro. The RNA polymerase activity of 1b was 6.4% of that of JFH1. In order to study the mechanism and identify domains responsible for the high polymerase activity of JFH1, we converted the amino acids of 1b RdRp to those of JFH1, and compared their Km, Vmax and template binding activity. Four amino acid mutations in the thumb domain of 1b RdRp, S377R, A450S, E455N and Y561F increased 1b polymerase activity, and their activity was 23.1, 45.8, 28.9, and 36.1% of JFH1, respectively. Vmax and RNA binding activity of JFH1, 1bwt and 1bA450S was JFH1 > 1bA450S > 1b, which indicated both high processivity and slightly higher template binding activity contributed to the high polymerase activity of JFH1. More... »

PAGES

765-773

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-009-0366-0

DOI

http://dx.doi.org/10.1007/s00705-009-0366-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046918363

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19340394


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1108", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Substitution", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hepacivirus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen-Ion Concentration", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kinetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA Replicase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Viral Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Virus Replication", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Chinese Academy of Sciences", 
          "id": "https://www.grid.ac/institutes/grid.9227.e", 
          "name": [
            "Unit of Viral Genome Regulation, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 225 South Chongqing Road, 200025, Shanghai, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Weng", 
        "givenName": "Leiyun", 
        "id": "sg:person.01202446653.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202446653.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shanghai Institutes for Biological Sciences", 
          "id": "https://www.grid.ac/institutes/grid.419092.7", 
          "name": [
            "State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, 200031, Shanghai, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Du", 
        "givenName": "Jiamu", 
        "id": "sg:person.01041510735.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01041510735.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chinese Academy of Sciences", 
          "id": "https://www.grid.ac/institutes/grid.9227.e", 
          "name": [
            "Unit of Viral Genome Regulation, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 225 South Chongqing Road, 200025, Shanghai, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhou", 
        "givenName": "Jingling", 
        "id": "sg:person.0660042320.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660042320.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Shanghai Institutes for Biological Sciences", 
          "id": "https://www.grid.ac/institutes/grid.419092.7", 
          "name": [
            "State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, 200031, Shanghai, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ding", 
        "givenName": "Jianping", 
        "id": "sg:person.0612512110.43", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612512110.43"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Infectious Diseases", 
          "id": "https://www.grid.ac/institutes/grid.410795.e", 
          "name": [
            "Department of Virology II, National Institute of Health, 1-23-1 Toyama, Shinjuku, 132-8640, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wakita", 
        "givenName": "Takaji", 
        "id": "sg:person.0671521055.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671521055.46"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Biology, 3-18-22 Honkomagome, Bunkyo-Ku, 113-8613, Tokyo, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kohara", 
        "givenName": "Michinori", 
        "id": "sg:person.01232206160.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232206160.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Chinese Academy of Sciences", 
          "id": "https://www.grid.ac/institutes/grid.9227.e", 
          "name": [
            "Unit of Viral Genome Regulation, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 225 South Chongqing Road, 200025, Shanghai, People\u2019s Republic of China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Toyoda", 
        "givenName": "Tetsuya", 
        "id": "sg:person.01116261170.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01116261170.05"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1073/pnas.96.23.13034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001183173"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/bbrc.2001.6333", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004014465"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.77.16.9020-9028.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004827259"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.78.3.1352-1366.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005321196"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0969-2126(00)80031-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005613212"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.74.4.2017-2022.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009749149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/viro.2001.0948", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021929174"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.02088-06", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022799357"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth0106-55", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024613012", 
          "https://doi.org/10.1038/nmeth0106-55"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nmeth0106-55", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024613012", 
          "https://doi.org/10.1038/nmeth0106-55"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.0014-2956.2001.02532.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027309269"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.74.23.11121-11128.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027849042"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.22.10773", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027852783"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.74.2.851-863.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028118039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jvi.74.19.9134-9143.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038417672"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/viro.1998.9311", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042123896"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm1268", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042875201", 
          "https://doi.org/10.1038/nm1268"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm1268", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042875201", 
          "https://doi.org/10.1038/nm1268"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m201251200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048988894"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m801490200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049564095"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/16.21.10339", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051239497"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075463755", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082785329", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1996.tb00329.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082875081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082884763", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083371858", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-05", 
    "datePublishedReg": "2009-05-01", 
    "description": "Hepatitis C virus (HCV) JFH1 efficiently replicates and produces infectious virus particles in cultured cells. We compared polymerase activity between JFH1 and 1b strains in vitro. The RNA polymerase activity of 1b was 6.4% of that of JFH1. In order to study the mechanism and identify domains responsible for the high polymerase activity of JFH1, we converted the amino acids of 1b RdRp to those of JFH1, and compared their Km, Vmax and template binding activity. Four amino acid mutations in the thumb domain of 1b RdRp, S377R, A450S, E455N and Y561F increased 1b polymerase activity, and their activity was 23.1, 45.8, 28.9, and 36.1% of JFH1, respectively. Vmax and RNA binding activity of JFH1, 1bwt and 1bA450S was JFH1 > 1bA450S > 1b, which indicated both high processivity and slightly higher template binding activity contributed to the high polymerase activity of JFH1.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00705-009-0366-0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083335", 
        "issn": [
          "0304-8608", 
          "1432-8798"
        ], 
        "name": "Archives of Virology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "154"
      }
    ], 
    "name": "Modification of hepatitis C virus 1b RNA polymerase to make a highly active JFH1-type polymerase by mutation of the thumb domain", 
    "pagination": "765-773", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "df8076ab4e2687f5e4df21d151aab0eae25fc6ec4cf4a9c50a581f3db7d6abe4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19340394"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "7506870"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00705-009-0366-0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1046918363"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00705-009-0366-0", 
      "https://app.dimensions.ai/details/publication/pub.1046918363"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T23:25", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000515.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00705-009-0366-0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00705-009-0366-0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00705-009-0366-0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00705-009-0366-0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00705-009-0366-0'


 

This table displays all metadata directly associated to this object as RDF triples.

229 TRIPLES      21 PREDICATES      63 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00705-009-0366-0 schema:about N20b02a9d5e424783a7fa4c294290ae89
2 N284c0ef691ab4df4b46167eeb19fd177
3 N2d4b313482ec48539f876871d1328b63
4 N3a1642623ada481f8ad325cf1a9132c9
5 N4bea3821eed44a6d97e02a6c8bc60f3d
6 N4ee513073ec9434cbd19347cf6ac657a
7 N5d79a5beccb84f1eb370e37c9fd50f7b
8 N7ac4adb93f364aa490ac371c40fd7659
9 Nb953de3053ae44739c04594942365f14
10 Nfa0a43ec39fc40538c4a18af443aa3db
11 anzsrc-for:11
12 anzsrc-for:1108
13 schema:author Nc8566013bdbc483895232db1e8835e5d
14 schema:citation sg:pub.10.1038/nm1268
15 sg:pub.10.1038/nmeth0106-55
16 https://app.dimensions.ai/details/publication/pub.1075463755
17 https://app.dimensions.ai/details/publication/pub.1082785329
18 https://app.dimensions.ai/details/publication/pub.1082884763
19 https://app.dimensions.ai/details/publication/pub.1083371858
20 https://doi.org/10.1002/j.1460-2075.1996.tb00329.x
21 https://doi.org/10.1006/bbrc.2001.6333
22 https://doi.org/10.1006/viro.1998.9311
23 https://doi.org/10.1006/viro.2001.0948
24 https://doi.org/10.1016/s0969-2126(00)80031-3
25 https://doi.org/10.1046/j.0014-2956.2001.02532.x
26 https://doi.org/10.1073/pnas.90.22.10773
27 https://doi.org/10.1073/pnas.96.23.13034
28 https://doi.org/10.1074/jbc.m201251200
29 https://doi.org/10.1074/jbc.m801490200
30 https://doi.org/10.1093/nar/16.21.10339
31 https://doi.org/10.1128/jvi.02088-06
32 https://doi.org/10.1128/jvi.74.19.9134-9143.2000
33 https://doi.org/10.1128/jvi.74.2.851-863.2000
34 https://doi.org/10.1128/jvi.74.23.11121-11128.2000
35 https://doi.org/10.1128/jvi.74.4.2017-2022.2000
36 https://doi.org/10.1128/jvi.77.16.9020-9028.2003
37 https://doi.org/10.1128/jvi.78.3.1352-1366.2004
38 schema:datePublished 2009-05
39 schema:datePublishedReg 2009-05-01
40 schema:description Hepatitis C virus (HCV) JFH1 efficiently replicates and produces infectious virus particles in cultured cells. We compared polymerase activity between JFH1 and 1b strains in vitro. The RNA polymerase activity of 1b was 6.4% of that of JFH1. In order to study the mechanism and identify domains responsible for the high polymerase activity of JFH1, we converted the amino acids of 1b RdRp to those of JFH1, and compared their Km, Vmax and template binding activity. Four amino acid mutations in the thumb domain of 1b RdRp, S377R, A450S, E455N and Y561F increased 1b polymerase activity, and their activity was 23.1, 45.8, 28.9, and 36.1% of JFH1, respectively. Vmax and RNA binding activity of JFH1, 1bwt and 1bA450S was JFH1 > 1bA450S > 1b, which indicated both high processivity and slightly higher template binding activity contributed to the high polymerase activity of JFH1.
41 schema:genre research_article
42 schema:inLanguage en
43 schema:isAccessibleForFree false
44 schema:isPartOf N2887f7aa13954bf393aee6fd95508172
45 Naf83065723e846b6b03156cefe527c57
46 sg:journal.1083335
47 schema:name Modification of hepatitis C virus 1b RNA polymerase to make a highly active JFH1-type polymerase by mutation of the thumb domain
48 schema:pagination 765-773
49 schema:productId N18741553946c414daaa76f2d1ede2eb4
50 N273b0d16c72b4c099ab9cf178bad7352
51 N50c3b9a1051d461a92b13fab2ef25ee3
52 N7d2088de22474ed5a17cdaa6421d74fc
53 Ne3482ada601e4bc8b425e5f76aec3a7b
54 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046918363
55 https://doi.org/10.1007/s00705-009-0366-0
56 schema:sdDatePublished 2019-04-10T23:25
57 schema:sdLicense https://scigraph.springernature.com/explorer/license/
58 schema:sdPublisher N8aee289c0f2b46448847ff1f779ca950
59 schema:url http://link.springer.com/10.1007%2Fs00705-009-0366-0
60 sgo:license sg:explorer/license/
61 sgo:sdDataset articles
62 rdf:type schema:ScholarlyArticle
63 N18741553946c414daaa76f2d1ede2eb4 schema:name doi
64 schema:value 10.1007/s00705-009-0366-0
65 rdf:type schema:PropertyValue
66 N1a4bbaea0081496ea7a172dd44a329d6 rdf:first sg:person.01116261170.05
67 rdf:rest rdf:nil
68 N20b02a9d5e424783a7fa4c294290ae89 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name RNA, Viral
70 rdf:type schema:DefinedTerm
71 N273b0d16c72b4c099ab9cf178bad7352 schema:name dimensions_id
72 schema:value pub.1046918363
73 rdf:type schema:PropertyValue
74 N284c0ef691ab4df4b46167eeb19fd177 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Mutation
76 rdf:type schema:DefinedTerm
77 N2887f7aa13954bf393aee6fd95508172 schema:issueNumber 5
78 rdf:type schema:PublicationIssue
79 N2d4b313482ec48539f876871d1328b63 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Hydrogen-Ion Concentration
81 rdf:type schema:DefinedTerm
82 N32b11cbea34a4917863864fd2ab6c284 rdf:first sg:person.0660042320.83
83 rdf:rest N75fb623804a74fdeace5dbffda8e635f
84 N3a1642623ada481f8ad325cf1a9132c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Virus Replication
86 rdf:type schema:DefinedTerm
87 N4bea3821eed44a6d97e02a6c8bc60f3d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name Viral Proteins
89 rdf:type schema:DefinedTerm
90 N4ee513073ec9434cbd19347cf6ac657a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Kinetics
92 rdf:type schema:DefinedTerm
93 N4fb21e1f327249cbb3ebcd59252b6f8e rdf:first sg:person.01232206160.76
94 rdf:rest N1a4bbaea0081496ea7a172dd44a329d6
95 N50c3b9a1051d461a92b13fab2ef25ee3 schema:name nlm_unique_id
96 schema:value 7506870
97 rdf:type schema:PropertyValue
98 N5d79a5beccb84f1eb370e37c9fd50f7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Hepacivirus
100 rdf:type schema:DefinedTerm
101 N739c5ff2e7ff42bcbe870339e7a3a6ed schema:name Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Biology, 3-18-22 Honkomagome, Bunkyo-Ku, 113-8613, Tokyo, Japan
102 rdf:type schema:Organization
103 N75fb623804a74fdeace5dbffda8e635f rdf:first sg:person.0612512110.43
104 rdf:rest Ncd37eb0ba9ea4a56bd17464bb2640aa4
105 N7ac4adb93f364aa490ac371c40fd7659 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Temperature
107 rdf:type schema:DefinedTerm
108 N7d2088de22474ed5a17cdaa6421d74fc schema:name readcube_id
109 schema:value df8076ab4e2687f5e4df21d151aab0eae25fc6ec4cf4a9c50a581f3db7d6abe4
110 rdf:type schema:PropertyValue
111 N8aee289c0f2b46448847ff1f779ca950 schema:name Springer Nature - SN SciGraph project
112 rdf:type schema:Organization
113 N9f8b5f2029bf4180a9f1f71c31e580ce rdf:first sg:person.01041510735.01
114 rdf:rest N32b11cbea34a4917863864fd2ab6c284
115 Naf83065723e846b6b03156cefe527c57 schema:volumeNumber 154
116 rdf:type schema:PublicationVolume
117 Nb953de3053ae44739c04594942365f14 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name RNA Replicase
119 rdf:type schema:DefinedTerm
120 Nc8566013bdbc483895232db1e8835e5d rdf:first sg:person.01202446653.25
121 rdf:rest N9f8b5f2029bf4180a9f1f71c31e580ce
122 Ncd37eb0ba9ea4a56bd17464bb2640aa4 rdf:first sg:person.0671521055.46
123 rdf:rest N4fb21e1f327249cbb3ebcd59252b6f8e
124 Ne3482ada601e4bc8b425e5f76aec3a7b schema:name pubmed_id
125 schema:value 19340394
126 rdf:type schema:PropertyValue
127 Nfa0a43ec39fc40538c4a18af443aa3db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Amino Acid Substitution
129 rdf:type schema:DefinedTerm
130 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
131 schema:name Medical and Health Sciences
132 rdf:type schema:DefinedTerm
133 anzsrc-for:1108 schema:inDefinedTermSet anzsrc-for:
134 schema:name Medical Microbiology
135 rdf:type schema:DefinedTerm
136 sg:journal.1083335 schema:issn 0304-8608
137 1432-8798
138 schema:name Archives of Virology
139 rdf:type schema:Periodical
140 sg:person.01041510735.01 schema:affiliation https://www.grid.ac/institutes/grid.419092.7
141 schema:familyName Du
142 schema:givenName Jiamu
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01041510735.01
144 rdf:type schema:Person
145 sg:person.01116261170.05 schema:affiliation https://www.grid.ac/institutes/grid.9227.e
146 schema:familyName Toyoda
147 schema:givenName Tetsuya
148 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01116261170.05
149 rdf:type schema:Person
150 sg:person.01202446653.25 schema:affiliation https://www.grid.ac/institutes/grid.9227.e
151 schema:familyName Weng
152 schema:givenName Leiyun
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202446653.25
154 rdf:type schema:Person
155 sg:person.01232206160.76 schema:affiliation N739c5ff2e7ff42bcbe870339e7a3a6ed
156 schema:familyName Kohara
157 schema:givenName Michinori
158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01232206160.76
159 rdf:type schema:Person
160 sg:person.0612512110.43 schema:affiliation https://www.grid.ac/institutes/grid.419092.7
161 schema:familyName Ding
162 schema:givenName Jianping
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612512110.43
164 rdf:type schema:Person
165 sg:person.0660042320.83 schema:affiliation https://www.grid.ac/institutes/grid.9227.e
166 schema:familyName Zhou
167 schema:givenName Jingling
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660042320.83
169 rdf:type schema:Person
170 sg:person.0671521055.46 schema:affiliation https://www.grid.ac/institutes/grid.410795.e
171 schema:familyName Wakita
172 schema:givenName Takaji
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671521055.46
174 rdf:type schema:Person
175 sg:pub.10.1038/nm1268 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042875201
176 https://doi.org/10.1038/nm1268
177 rdf:type schema:CreativeWork
178 sg:pub.10.1038/nmeth0106-55 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024613012
179 https://doi.org/10.1038/nmeth0106-55
180 rdf:type schema:CreativeWork
181 https://app.dimensions.ai/details/publication/pub.1075463755 schema:CreativeWork
182 https://app.dimensions.ai/details/publication/pub.1082785329 schema:CreativeWork
183 https://app.dimensions.ai/details/publication/pub.1082884763 schema:CreativeWork
184 https://app.dimensions.ai/details/publication/pub.1083371858 schema:CreativeWork
185 https://doi.org/10.1002/j.1460-2075.1996.tb00329.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082875081
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1006/bbrc.2001.6333 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004014465
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1006/viro.1998.9311 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042123896
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1006/viro.2001.0948 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021929174
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/s0969-2126(00)80031-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005613212
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1046/j.0014-2956.2001.02532.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1027309269
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1073/pnas.90.22.10773 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027852783
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1073/pnas.96.23.13034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001183173
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1074/jbc.m201251200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048988894
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1074/jbc.m801490200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049564095
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1093/nar/16.21.10339 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051239497
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1128/jvi.02088-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022799357
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1128/jvi.74.19.9134-9143.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038417672
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1128/jvi.74.2.851-863.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028118039
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1128/jvi.74.23.11121-11128.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027849042
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1128/jvi.74.4.2017-2022.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009749149
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1128/jvi.77.16.9020-9028.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004827259
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1128/jvi.78.3.1352-1366.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005321196
220 rdf:type schema:CreativeWork
221 https://www.grid.ac/institutes/grid.410795.e schema:alternateName National Institute of Infectious Diseases
222 schema:name Department of Virology II, National Institute of Health, 1-23-1 Toyama, Shinjuku, 132-8640, Tokyo, Japan
223 rdf:type schema:Organization
224 https://www.grid.ac/institutes/grid.419092.7 schema:alternateName Shanghai Institutes for Biological Sciences
225 schema:name State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, 200031, Shanghai, China
226 rdf:type schema:Organization
227 https://www.grid.ac/institutes/grid.9227.e schema:alternateName Chinese Academy of Sciences
228 schema:name Unit of Viral Genome Regulation, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 225 South Chongqing Road, 200025, Shanghai, People’s Republic of China
229 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...