Efficient production of type 2 porcine circovirus-like particles by a recombinant baculovirus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-12

AUTHORS

Lan-Jun Liu, Takako Suzuki, Hiroshi Tsunemitsu, Michiyo Kataoka, Noriyo Ngata, Naokazu Takeda, Takaji Wakita, Tatsuo Miyamura, Tian-Cheng Li

ABSTRACT

The capsid protein of PCV2 was expressed by using a recombinant baculovirus with insect Tn5 cells. A large amount of 28-kDa protein was released into the culture medium and self-assembled into PCV2-like particles (PCV2-LPs) with a buoyant density of 1.365 g/cm(3) and a diameter of 20 nm. PCV2-LPs were efficiently expressed, yielding 1 mg of purified particles per 10(7) Tn5 cells. The PCV2-LPs have antigenicity similar to that of authentic PCV2 particles, allowing us to develop a method for sensitively detecting PCV2-specific IgG antibodies. In addition, the PCV2-LPs appeared to be the most promising PCV2 vaccine candidate, by virtue of their potent immunogenicity. More... »

PAGES

2291

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-008-0248-x

DOI

http://dx.doi.org/10.1007/s00705-008-0248-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035287749

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18998045


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