Bcl-2 inhibits the caspase-dependent apoptosis induced by SARS-CoV without affecting virus replication kinetics View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2005-09-12

AUTHORS

L. Bordi, C. Castilletti, L. Falasca, F. Ciccosanti, S. Calcaterra, G. Rozera, A. Di Caro, S. Zaniratti, A. Rinaldi, G. Ippolito, M. Piacentini, M. R. Capobianchi

ABSTRACT

Vero cells transfected with either neo- or bcl-2-plasmid were infected with SARS-CoV at a high multiplicity of infection. Apoptosis appeared after the onset of CPE and completion of virus replication, and could be prevented by Bcl-2 expression. Apoptosis is likely mediated by the mitochondrial pathway, as demonstrated by its inhibition using Bcl-2, and by the activation of the caspase cascade, resulting in PARP cleavage. Prevention of apoptosis did not affect susceptibility to infection, kinetics and extent of viral replication and release, thus implying that apoptosis is not involved in facilitating release and/or dissemination of SARS-CoV in Vero cells. More... »

PAGES

369-377

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00705-005-0632-8

DOI

http://dx.doi.org/10.1007/s00705-005-0632-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037602016

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16155806


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