Alzheimer’s senile plaque as shown by microcryodissection, a new technique for dissociating tissue structures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-04-06

AUTHORS

Manon Thierry, Serge Marty, Susana Boluda, Charles Duyckaerts

ABSTRACT

Extracellular accumulation of Aβ peptides and intracellular aggregation of hyperphosphorylated tau proteins are the two hallmark lesions of Alzheimer disease (AD). The senile plaque is made of a core of extracellular Aβ surrounded by phospho-tau positive neurites. It includes multiple components such as axons, synapses, glial fibers and microglia. To visualize the relationships of those elements, an original technique was developed, based on the dilation of interstitial water during freezing. Samples of neocortex, hippocampus and striatum were taken from formalin-fixed brains (one control case; three cases with severe Alzheimer disease). The samples were subjected to various numbers of freezing/thawing cycles (from 0 to 320) with an automated system we devised. The samples were embedded in paraffin, cut and stained with haematoxylin-eosin or immunostained against Aβ, phospho-tau, and antigens enriched in axons, synapses, macrophages or astrocytes. Microcryodissection induced the dissociation of tissue components, especially in the grey matter where the neuropil formed an oriented “mesh”. The size of the empty spaces separating the fiber bundles and cells increased with the number of cycles. The amyloid core of the senile plaque separated from its neuritic crown at around 300 freezing/thawing cycles. The dissected core remained associated with macrophages containing Aβ in their cytoplasm. Phospho-tau positive axons were distinctly seen projecting from the neuritic crown to the isolated amyloid core, where they ended in large synapses. The microcryodissection showed astrocytic processes stuck directly to the core. The original method we developed—microcryodissection—helped understanding how histological components were assembled in the tissue. More... »

PAGES

685-694

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00702-017-1718-7

DOI

http://dx.doi.org/10.1007/s00702-017-1718-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084518036

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28386671


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aged", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aged, 80 and over", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alzheimer Disease", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Astrocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Brain", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cryopreservation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Female", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Freezing", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunohistochemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Macrophages", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Male", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mice", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microdissection", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Microscopy, Electron", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Middle Aged", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Neurons", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pattern Recognition, Automated", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plaque, Amyloid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Water", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Laboratoire de Neuropathologie Raymond ESCOUROLLE, Paris, France", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Alzheimer\u2019s and Prion Diseases Team, Institut du Cerveau et de la Moelle Epini\u00e8re, CNRS UMR 7225, INSERM 1127, UPMC UM75, Paris, France", 
            "Laboratoire de Neuropathologie Raymond ESCOUROLLE, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thierry", 
        "givenName": "Manon", 
        "id": "sg:person.010114420263.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010114420263.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Alzheimer\u2019s and Prion Diseases Team, Institut du Cerveau et de la Moelle Epini\u00e8re, CNRS UMR 7225, INSERM 1127, UPMC UM75, Paris, France", 
          "id": "http://www.grid.ac/institutes/grid.425274.2", 
          "name": [
            "Alzheimer\u2019s and Prion Diseases Team, Institut du Cerveau et de la Moelle Epini\u00e8re, CNRS UMR 7225, INSERM 1127, UPMC UM75, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Marty", 
        "givenName": "Serge", 
        "id": "sg:person.0732652424.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732652424.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratoire de Neuropathologie Raymond ESCOUROLLE, Paris, France", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Alzheimer\u2019s and Prion Diseases Team, Institut du Cerveau et de la Moelle Epini\u00e8re, CNRS UMR 7225, INSERM 1127, UPMC UM75, Paris, France", 
            "Laboratoire de Neuropathologie Raymond ESCOUROLLE, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Boluda", 
        "givenName": "Susana", 
        "id": "sg:person.011507361263.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011507361263.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Laboratoire de Neuropathologie Raymond ESCOUROLLE, Paris, France", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Alzheimer\u2019s and Prion Diseases Team, Institut du Cerveau et de la Moelle Epini\u00e8re, CNRS UMR 7225, INSERM 1127, UPMC UM75, Paris, France", 
            "Laboratoire de Neuropathologie Raymond ESCOUROLLE, Paris, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Duyckaerts", 
        "givenName": "Charles", 
        "id": "sg:person.01366054505.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366054505.04"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s00401-009-0532-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022622684", 
          "https://doi.org/10.1007/s00401-009-0532-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00401-015-1516-y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010599607", 
          "https://doi.org/10.1007/s00401-015-1516-y"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00227813", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049587307", 
          "https://doi.org/10.1007/bf00227813"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00401-014-1356-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008707168", 
          "https://doi.org/10.1007/s00401-014-1356-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00308809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000355620", 
          "https://doi.org/10.1007/bf00308809"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-04-06", 
    "datePublishedReg": "2017-04-06", 
    "description": "Extracellular accumulation of A\u03b2 peptides and intracellular aggregation of hyperphosphorylated tau proteins are the two hallmark lesions of Alzheimer disease (AD). The senile plaque is made of a core of extracellular A\u03b2 surrounded by phospho-tau positive neurites. It includes multiple components such as axons, synapses, glial fibers and microglia. To visualize the relationships of those elements, an original technique was developed, based on the dilation of interstitial water during freezing. Samples of neocortex, hippocampus and striatum were taken from formalin-fixed brains (one control case; three cases with severe Alzheimer disease). The samples were subjected to various numbers of freezing/thawing cycles (from 0 to 320) with an automated system we devised. The samples were embedded in paraffin, cut and stained with haematoxylin-eosin or immunostained against A\u03b2, phospho-tau, and antigens enriched in axons, synapses, macrophages or astrocytes. Microcryodissection induced the dissociation of tissue components, especially in the grey matter where the neuropil formed an oriented \u201cmesh\u201d. The size of the empty spaces separating the fiber bundles and cells increased with the number of cycles. The amyloid core of the senile plaque separated from its neuritic crown at around 300 freezing/thawing cycles. The dissected core remained associated with macrophages containing A\u03b2 in their cytoplasm. Phospho-tau positive axons were distinctly seen projecting from the neuritic crown to the isolated amyloid core, where they ended in large synapses. The microcryodissection showed astrocytic processes stuck directly to the core. The original method we developed\u2014microcryodissection\u2014helped understanding how histological components were assembled in the tissue.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s00702-017-1718-7", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1412779", 
        "issn": [
          "0300-9564", 
          "1435-1463"
        ], 
        "name": "Journal of Neural Transmission", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "124"
      }
    ], 
    "keywords": [
      "senile plaques", 
      "Alzheimer's disease", 
      "Alzheimer senile plaques", 
      "samples of neocortex", 
      "amyloid core", 
      "phospho-tau", 
      "positive neurites", 
      "positive axons", 
      "extracellular A\u03b2", 
      "hallmark lesions", 
      "large synapses", 
      "glial fibers", 
      "astrocytic processes", 
      "gray matter", 
      "tau protein", 
      "histological components", 
      "plaques", 
      "axons", 
      "extracellular accumulation", 
      "synapses", 
      "A\u03b2 peptides", 
      "tissue components", 
      "macrophages", 
      "A\u03b2", 
      "intracellular aggregation", 
      "tissue structure", 
      "microglia", 
      "hippocampus", 
      "striatum", 
      "astrocytes", 
      "lesions", 
      "neuropil", 
      "neocortex", 
      "antigen", 
      "disease", 
      "original technique", 
      "brain", 
      "formalin", 
      "dilation", 
      "neurites", 
      "tissue", 
      "paraffin", 
      "cells", 
      "fiber bundles", 
      "multiple components", 
      "samples", 
      "peptides", 
      "crowns", 
      "cytoplasm", 
      "new technique", 
      "protein", 
      "number", 
      "accumulation", 
      "cycle", 
      "bundles", 
      "components", 
      "relationship", 
      "automated system", 
      "fibers", 
      "technique", 
      "freezing/", 
      "understanding", 
      "aggregation", 
      "dissociation", 
      "freezing", 
      "size", 
      "method", 
      "number of cycles", 
      "system", 
      "matter", 
      "empty space", 
      "original method", 
      "process", 
      "mesh", 
      "core", 
      "elements", 
      "space", 
      "water", 
      "structure", 
      "interstitial water"
    ], 
    "name": "Alzheimer\u2019s senile plaque as shown by microcryodissection, a new technique for dissociating tissue structures", 
    "pagination": "685-694", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1084518036"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00702-017-1718-7"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28386671"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00702-017-1718-7", 
      "https://app.dimensions.ai/details/publication/pub.1084518036"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:36", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_726.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s00702-017-1718-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00702-017-1718-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00702-017-1718-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00702-017-1718-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00702-017-1718-7'


 

This table displays all metadata directly associated to this object as RDF triples.

270 TRIPLES      21 PREDICATES      131 URIs      118 LITERALS      28 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00702-017-1718-7 schema:about N0c50815bad6d468c826f89b2159ed65c
2 N1c4aa4e025be45199d8cf8e800871c15
3 N238fbb5031f94e1cb692d30807a4bf66
4 N266a8900581a43259419c730c42ece38
5 N26b07734d061417dab0b1b33b1a2bf41
6 N2e3955194e8c4aaf9e6f70c7f7a85417
7 N50ea0155882440e0bc3dd167944993a4
8 N5485cc1e8c1c4371a5c696a508c9e95c
9 N5766532b6af34a168d547a01c760fa2b
10 N57839d03cb7f469dadb5330bfbd20cc0
11 N6e742c79037845edaee8958af1b60635
12 N6fe6665d0e7244af8a521752a931578f
13 N719e12d25ee446c886532557746e240d
14 N88f334f926004cad85e5e6a2f1bd7adb
15 N92b4e24b77094256875f282181a41d57
16 Nb080a1fa59b44048a4c1c08955145ba4
17 Nde09fa1931854b46bd37bca8650ac4e3
18 Nf105d2661b4645d1ab5f867ee19fd6bd
19 Nf187dd9c62dd413dbe1a0458e77f5718
20 Nf6e5c7c74aa9495c8d0071866bb5e055
21 Nf909080681d94e698efb55515a2e4bec
22 anzsrc-for:11
23 anzsrc-for:1109
24 schema:author Nd2b878a01d7143cfa5f513da6335ecbf
25 schema:citation sg:pub.10.1007/bf00227813
26 sg:pub.10.1007/bf00308809
27 sg:pub.10.1007/s00401-009-0532-1
28 sg:pub.10.1007/s00401-014-1356-1
29 sg:pub.10.1007/s00401-015-1516-y
30 schema:datePublished 2017-04-06
31 schema:datePublishedReg 2017-04-06
32 schema:description Extracellular accumulation of Aβ peptides and intracellular aggregation of hyperphosphorylated tau proteins are the two hallmark lesions of Alzheimer disease (AD). The senile plaque is made of a core of extracellular Aβ surrounded by phospho-tau positive neurites. It includes multiple components such as axons, synapses, glial fibers and microglia. To visualize the relationships of those elements, an original technique was developed, based on the dilation of interstitial water during freezing. Samples of neocortex, hippocampus and striatum were taken from formalin-fixed brains (one control case; three cases with severe Alzheimer disease). The samples were subjected to various numbers of freezing/thawing cycles (from 0 to 320) with an automated system we devised. The samples were embedded in paraffin, cut and stained with haematoxylin-eosin or immunostained against Aβ, phospho-tau, and antigens enriched in axons, synapses, macrophages or astrocytes. Microcryodissection induced the dissociation of tissue components, especially in the grey matter where the neuropil formed an oriented “mesh”. The size of the empty spaces separating the fiber bundles and cells increased with the number of cycles. The amyloid core of the senile plaque separated from its neuritic crown at around 300 freezing/thawing cycles. The dissected core remained associated with macrophages containing Aβ in their cytoplasm. Phospho-tau positive axons were distinctly seen projecting from the neuritic crown to the isolated amyloid core, where they ended in large synapses. The microcryodissection showed astrocytic processes stuck directly to the core. The original method we developed—microcryodissection—helped understanding how histological components were assembled in the tissue.
33 schema:genre article
34 schema:isAccessibleForFree false
35 schema:isPartOf N56ca1a5300484fffa53cadcb68e258ec
36 Nf0d35cfde01d45ba8137fb2f044731b4
37 sg:journal.1412779
38 schema:keywords Alzheimer senile plaques
39 Alzheimer's disease
40
41 Aβ peptides
42 accumulation
43 aggregation
44 amyloid core
45 antigen
46 astrocytes
47 astrocytic processes
48 automated system
49 axons
50 brain
51 bundles
52 cells
53 components
54 core
55 crowns
56 cycle
57 cytoplasm
58 dilation
59 disease
60 dissociation
61 elements
62 empty space
63 extracellular Aβ
64 extracellular accumulation
65 fiber bundles
66 fibers
67 formalin
68 freezing
69 freezing/
70 glial fibers
71 gray matter
72 hallmark lesions
73 hippocampus
74 histological components
75 interstitial water
76 intracellular aggregation
77 large synapses
78 lesions
79 macrophages
80 matter
81 mesh
82 method
83 microglia
84 multiple components
85 neocortex
86 neurites
87 neuropil
88 new technique
89 number
90 number of cycles
91 original method
92 original technique
93 paraffin
94 peptides
95 phospho-tau
96 plaques
97 positive axons
98 positive neurites
99 process
100 protein
101 relationship
102 samples
103 samples of neocortex
104 senile plaques
105 size
106 space
107 striatum
108 structure
109 synapses
110 system
111 tau protein
112 technique
113 tissue
114 tissue components
115 tissue structure
116 understanding
117 water
118 schema:name Alzheimer’s senile plaque as shown by microcryodissection, a new technique for dissociating tissue structures
119 schema:pagination 685-694
120 schema:productId N427ec85cc39845b2a422d4e762c7727b
121 N6b29b9be63174e3995b57893f0473c8f
122 Nb9c4cfa57a86438e961acbdcefb6475e
123 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084518036
124 https://doi.org/10.1007/s00702-017-1718-7
125 schema:sdDatePublished 2022-12-01T06:36
126 schema:sdLicense https://scigraph.springernature.com/explorer/license/
127 schema:sdPublisher N1675d15ae1484b548943b11776724366
128 schema:url https://doi.org/10.1007/s00702-017-1718-7
129 sgo:license sg:explorer/license/
130 sgo:sdDataset articles
131 rdf:type schema:ScholarlyArticle
132 N0c50815bad6d468c826f89b2159ed65c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Cryopreservation
134 rdf:type schema:DefinedTerm
135 N11d73c12c03344318d7e193cb281ad29 rdf:first sg:person.01366054505.04
136 rdf:rest rdf:nil
137 N1675d15ae1484b548943b11776724366 schema:name Springer Nature - SN SciGraph project
138 rdf:type schema:Organization
139 N1c4aa4e025be45199d8cf8e800871c15 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Immunohistochemistry
141 rdf:type schema:DefinedTerm
142 N1c57fbf8fc7b4793bc9fce168473e6de rdf:first sg:person.011507361263.54
143 rdf:rest N11d73c12c03344318d7e193cb281ad29
144 N238fbb5031f94e1cb692d30807a4bf66 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Animals
146 rdf:type schema:DefinedTerm
147 N266a8900581a43259419c730c42ece38 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Mice
149 rdf:type schema:DefinedTerm
150 N26b07734d061417dab0b1b33b1a2bf41 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Male
152 rdf:type schema:DefinedTerm
153 N2e3955194e8c4aaf9e6f70c7f7a85417 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
154 schema:name Freezing
155 rdf:type schema:DefinedTerm
156 N427ec85cc39845b2a422d4e762c7727b schema:name doi
157 schema:value 10.1007/s00702-017-1718-7
158 rdf:type schema:PropertyValue
159 N50ea0155882440e0bc3dd167944993a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Macrophages
161 rdf:type schema:DefinedTerm
162 N5485cc1e8c1c4371a5c696a508c9e95c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name Neurons
164 rdf:type schema:DefinedTerm
165 N56ca1a5300484fffa53cadcb68e258ec schema:volumeNumber 124
166 rdf:type schema:PublicationVolume
167 N5766532b6af34a168d547a01c760fa2b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Microscopy, Electron
169 rdf:type schema:DefinedTerm
170 N57839d03cb7f469dadb5330bfbd20cc0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Pattern Recognition, Automated
172 rdf:type schema:DefinedTerm
173 N6b29b9be63174e3995b57893f0473c8f schema:name pubmed_id
174 schema:value 28386671
175 rdf:type schema:PropertyValue
176 N6e742c79037845edaee8958af1b60635 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
177 schema:name Astrocytes
178 rdf:type schema:DefinedTerm
179 N6fe6665d0e7244af8a521752a931578f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
180 schema:name Aged
181 rdf:type schema:DefinedTerm
182 N719e12d25ee446c886532557746e240d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
183 schema:name Brain
184 rdf:type schema:DefinedTerm
185 N88f334f926004cad85e5e6a2f1bd7adb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
186 schema:name Microdissection
187 rdf:type schema:DefinedTerm
188 N92b4e24b77094256875f282181a41d57 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Alzheimer Disease
190 rdf:type schema:DefinedTerm
191 Nb080a1fa59b44048a4c1c08955145ba4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Humans
193 rdf:type schema:DefinedTerm
194 Nb9c4cfa57a86438e961acbdcefb6475e schema:name dimensions_id
195 schema:value pub.1084518036
196 rdf:type schema:PropertyValue
197 Nd2b878a01d7143cfa5f513da6335ecbf rdf:first sg:person.010114420263.50
198 rdf:rest Ndfaa757445e8497c97160d63258d812b
199 Nde09fa1931854b46bd37bca8650ac4e3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
200 schema:name Plaque, Amyloid
201 rdf:type schema:DefinedTerm
202 Ndfaa757445e8497c97160d63258d812b rdf:first sg:person.0732652424.06
203 rdf:rest N1c57fbf8fc7b4793bc9fce168473e6de
204 Nf0d35cfde01d45ba8137fb2f044731b4 schema:issueNumber 6
205 rdf:type schema:PublicationIssue
206 Nf105d2661b4645d1ab5f867ee19fd6bd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
207 schema:name Water
208 rdf:type schema:DefinedTerm
209 Nf187dd9c62dd413dbe1a0458e77f5718 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
210 schema:name Aged, 80 and over
211 rdf:type schema:DefinedTerm
212 Nf6e5c7c74aa9495c8d0071866bb5e055 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
213 schema:name Middle Aged
214 rdf:type schema:DefinedTerm
215 Nf909080681d94e698efb55515a2e4bec schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
216 schema:name Female
217 rdf:type schema:DefinedTerm
218 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
219 schema:name Medical and Health Sciences
220 rdf:type schema:DefinedTerm
221 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
222 schema:name Neurosciences
223 rdf:type schema:DefinedTerm
224 sg:journal.1412779 schema:issn 0300-9564
225 1435-1463
226 schema:name Journal of Neural Transmission
227 schema:publisher Springer Nature
228 rdf:type schema:Periodical
229 sg:person.010114420263.50 schema:affiliation grid-institutes:None
230 schema:familyName Thierry
231 schema:givenName Manon
232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010114420263.50
233 rdf:type schema:Person
234 sg:person.011507361263.54 schema:affiliation grid-institutes:None
235 schema:familyName Boluda
236 schema:givenName Susana
237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011507361263.54
238 rdf:type schema:Person
239 sg:person.01366054505.04 schema:affiliation grid-institutes:None
240 schema:familyName Duyckaerts
241 schema:givenName Charles
242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366054505.04
243 rdf:type schema:Person
244 sg:person.0732652424.06 schema:affiliation grid-institutes:grid.425274.2
245 schema:familyName Marty
246 schema:givenName Serge
247 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732652424.06
248 rdf:type schema:Person
249 sg:pub.10.1007/bf00227813 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049587307
250 https://doi.org/10.1007/bf00227813
251 rdf:type schema:CreativeWork
252 sg:pub.10.1007/bf00308809 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000355620
253 https://doi.org/10.1007/bf00308809
254 rdf:type schema:CreativeWork
255 sg:pub.10.1007/s00401-009-0532-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022622684
256 https://doi.org/10.1007/s00401-009-0532-1
257 rdf:type schema:CreativeWork
258 sg:pub.10.1007/s00401-014-1356-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008707168
259 https://doi.org/10.1007/s00401-014-1356-1
260 rdf:type schema:CreativeWork
261 sg:pub.10.1007/s00401-015-1516-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1010599607
262 https://doi.org/10.1007/s00401-015-1516-y
263 rdf:type schema:CreativeWork
264 grid-institutes:None schema:alternateName Laboratoire de Neuropathologie Raymond ESCOUROLLE, Paris, France
265 schema:name Alzheimer’s and Prion Diseases Team, Institut du Cerveau et de la Moelle Epinière, CNRS UMR 7225, INSERM 1127, UPMC UM75, Paris, France
266 Laboratoire de Neuropathologie Raymond ESCOUROLLE, Paris, France
267 rdf:type schema:Organization
268 grid-institutes:grid.425274.2 schema:alternateName Alzheimer’s and Prion Diseases Team, Institut du Cerveau et de la Moelle Epinière, CNRS UMR 7225, INSERM 1127, UPMC UM75, Paris, France
269 schema:name Alzheimer’s and Prion Diseases Team, Institut du Cerveau et de la Moelle Epinière, CNRS UMR 7225, INSERM 1127, UPMC UM75, Paris, France
270 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...