Ontology type: schema:ScholarlyArticle
2017-03-18
AUTHORSZoya Marinova, Susanne Walitza, Edna Grünblatt
ABSTRACTDecreased neurotrophic factors expression and neurotrophin receptors signalling have repeatedly been reported in association with stress, depression, and neurodegenerative disorders. We have previously identified the hallucinogen 2,5-dimethoxy-4-iodoamphetamine hydrochloride (DOI) as protective against trophic deprivation-induced cytotoxicity in human neuroblastoma SK-N-SH cells and established the dependence of this effect on the 5-HT2A receptor, tyrosine kinases activity, and the extracellular signal-regulated kinase pathway. In the current study, we investigated the effect of DOI on tropomyosin-related kinase receptor A (TrkA) phosphorylation. Treatment with DOI increased TrkA tyrosine phosphorylation in SK-N-SH cells, determined by immunoprecipitation with TrkA antibody and immunoblotting with anti-phosphotyrosine- and TrkA-antibodies. Analysis of DOI’s effect on individual TrkA residues in SK-N-SH cells showed that it increases TrkA Tyr490 phosphorylation (177 ± 23% after 5 μM DOI for 30 min compared to vehicle). Furthermore, DOI treatment increased the percentage of SK-N-SH cells extending neurites in a TrkA-dependent manner (17.2 ± 2.2% after 5 μM DOI treatment for 6 days compared to 5.6 ± 1.7% after vehicle). In a different cell model—lymphoblastoid cell lines—DOI treatment increased tropomyosin-related kinase receptor B (TrkB) phosphorylation, determined by immunoprecipitation with TrkB antibody and immunoblotting with anti-phosphotyrosine antibody and total Trk antibody. Our results identify the Trk receptors as a downstream target of the hallucinogen DOI. In light of the known involvement of Trk receptors in mental diseases, their participation in DOI-mediated effects warrants further investigation. More... »
PAGES749-759
http://scigraph.springernature.com/pub.10.1007/s00702-017-1706-y
DOIhttp://dx.doi.org/10.1007/s00702-017-1706-y
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1084023046
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/28315978
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Neurosciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Amphetamines",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Line, Tumor",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Hallucinogens",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Lymphocytes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Male",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Nerve Growth Factor",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Neurites",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Neuronal Outgrowth",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Phosphorylation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Receptor, Serotonin, 5-HT2A",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Receptor, Serotonin, 5-HT2B",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Receptor, trkA",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Receptor, trkB",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Serotonin 5-HT2 Receptor Antagonists",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Young Adult",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Department of Psychosomatic Medicine, Clinic Barmelweid, 5017, Barmelweid, Switzerland",
"id": "http://www.grid.ac/institutes/grid.452327.5",
"name": [
"Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric Hospital, University of Z\u00fcrich, Wagistrasse 12, Schlieren, 8952, Zurich, Switzerland",
"Department of Psychosomatic Medicine, Clinic Barmelweid, 5017, Barmelweid, Switzerland"
],
"type": "Organization"
},
"familyName": "Marinova",
"givenName": "Zoya",
"id": "sg:person.0755076545.30",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0755076545.30"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Z\u00fcrich Center for Integrative Human Physiology, University of Z\u00fcrich, Zurich, Switzerland",
"id": "http://www.grid.ac/institutes/grid.7400.3",
"name": [
"Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric Hospital, University of Z\u00fcrich, Wagistrasse 12, Schlieren, 8952, Zurich, Switzerland",
"Neuroscience Center Z\u00fcrich, University of Z\u00fcrich and ETH Z\u00fcrich, Zurich, Switzerland",
"Z\u00fcrich Center for Integrative Human Physiology, University of Z\u00fcrich, Zurich, Switzerland"
],
"type": "Organization"
},
"familyName": "Walitza",
"givenName": "Susanne",
"id": "sg:person.0736321374.53",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736321374.53"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Z\u00fcrich Center for Integrative Human Physiology, University of Z\u00fcrich, Zurich, Switzerland",
"id": "http://www.grid.ac/institutes/grid.7400.3",
"name": [
"Department of Child and Adolescent Psychiatry and Psychotherapy, Psychiatric Hospital, University of Z\u00fcrich, Wagistrasse 12, Schlieren, 8952, Zurich, Switzerland",
"Neuroscience Center Z\u00fcrich, University of Z\u00fcrich and ETH Z\u00fcrich, Zurich, Switzerland",
"Z\u00fcrich Center for Integrative Human Physiology, University of Z\u00fcrich, Zurich, Switzerland"
],
"type": "Organization"
},
"familyName": "Gr\u00fcnblatt",
"givenName": "Edna",
"id": "sg:person.01126412736.07",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01126412736.07"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1385/nmm:1:3:197",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036553133",
"https://doi.org/10.1385/nmm:1:3:197"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1479-5876-10-239",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023364217",
"https://doi.org/10.1186/1479-5876-10-239"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/gb-2007-8-2-r19",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007077528",
"https://doi.org/10.1186/gb-2007-8-2-r19"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00018-006-6010-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046148415",
"https://doi.org/10.1007/s00018-006-6010-1"
],
"type": "CreativeWork"
}
],
"datePublished": "2017-03-18",
"datePublishedReg": "2017-03-18",
"description": "Decreased neurotrophic factors expression and neurotrophin receptors signalling have repeatedly been reported in association with stress, depression, and neurodegenerative disorders. We have previously identified the hallucinogen 2,5-dimethoxy-4-iodoamphetamine hydrochloride (DOI) as protective against trophic deprivation-induced cytotoxicity in human neuroblastoma SK-N-SH cells and established the dependence of this effect on the 5-HT2A receptor, tyrosine kinases activity, and the extracellular signal-regulated kinase pathway. In the current study, we investigated the effect of DOI on tropomyosin-related kinase receptor A (TrkA) phosphorylation. Treatment with DOI increased TrkA tyrosine phosphorylation in SK-N-SH cells, determined by immunoprecipitation with TrkA antibody and immunoblotting with anti-phosphotyrosine- and TrkA-antibodies. Analysis of DOI\u2019s effect on individual TrkA residues in SK-N-SH cells showed that it increases TrkA Tyr490 phosphorylation (177\u00a0\u00b1\u00a023% after 5\u00a0\u03bcM DOI for 30\u00a0min compared to vehicle). Furthermore, DOI treatment increased the percentage of SK-N-SH cells extending neurites in a TrkA-dependent manner (17.2\u00a0\u00b1\u00a02.2% after 5\u00a0\u03bcM DOI treatment for 6\u00a0days compared to 5.6\u00a0\u00b1\u00a01.7% after vehicle). In a different cell model\u2014lymphoblastoid cell lines\u2014DOI treatment increased tropomyosin-related kinase receptor B (TrkB) phosphorylation, determined by immunoprecipitation with TrkB antibody and immunoblotting with anti-phosphotyrosine antibody and total Trk antibody. Our results identify the Trk receptors as a downstream target of the hallucinogen DOI. In light of the known involvement of Trk receptors in mental diseases, their participation in DOI-mediated effects warrants further investigation.",
"genre": "article",
"id": "sg:pub.10.1007/s00702-017-1706-y",
"isAccessibleForFree": false,
"isFundedItemOf": [
{
"id": "sg:grant.5221586",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.5238123",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1412779",
"issn": [
"0300-9564",
"1435-1463"
],
"name": "Journal of Neural Transmission",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "6",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "124"
}
],
"keywords": [
"SK-N-SH cells",
"neurotrophin receptor",
"Trk receptors",
"TrkA antibody",
"effects warrants further investigation",
"neurotrophic factor expression",
"neuroblastoma SK-N-SH cells",
"human neuroblastoma SK-N-SH cells",
"effect of DOI",
"TrkA tyrosine phosphorylation",
"neuronal cell line",
"warrants further investigation",
"DOI treatment",
"deprivation-induced cytotoxicity",
"TrkB antibody",
"extracellular signal-regulated kinase (ERK) pathway",
"signal-regulated kinase pathway",
"Trk antibody",
"hallucinogen DOI",
"neurodegenerative disorders",
"factor expression",
"TrkA-dependent manner",
"tyrosine kinase activity",
"receptors",
"antibodies",
"mental diseases",
"cell lines",
"anti-phosphotyrosine antibody",
"treatment",
"further investigation",
"downstream targets",
"kinase pathway",
"B phosphorylation",
"hallucinogens",
"cells",
"current study",
"A Phosphorylation",
"tyrosine phosphorylation",
"phosphorylation",
"kinase activity",
"immunoprecipitation",
"disease",
"hydrochloride",
"disorders",
"depression",
"neurites",
"effect",
"DOI effect",
"association",
"cytotoxicity",
"involvement",
"expression",
"pathway",
"percentage",
"promotes",
"target",
"activity",
"study",
"manner",
"stress",
"participation",
"lines",
"investigation",
"DOI",
"analysis",
"results",
"light",
"extension",
"residues",
"dependence"
],
"name": "The hallucinogen 2,5-dimethoxy-4-iodoamphetamine hydrochloride activates neurotrophin receptors in a neuronal cell line and promotes neurites extension",
"pagination": "749-759",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1084023046"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00702-017-1706-y"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"28315978"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00702-017-1706-y",
"https://app.dimensions.ai/details/publication/pub.1084023046"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T17:06",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_729.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s00702-017-1706-y"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00702-017-1706-y'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00702-017-1706-y'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00702-017-1706-y'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00702-017-1706-y'
This table displays all metadata directly associated to this object as RDF triples.
235 TRIPLES
21 PREDICATES
115 URIs
103 LITERALS
23 BLANK NODES