Solid-phase microbead array for multiplex O-serotyping of Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-05

AUTHORS

Claudia Liebsch, Stefan Rödiger, Alexander Böhm, Jörg Nitschke, Jörg Weinreich, Angelika Fruth, Dirk Roggenbuck, Werner Lehmann, Uwe Schedler, Thomas Juretzek, Peter Schierack

ABSTRACT

Typing and classification of Escherichia coli (E. coli) according to cell wall components, like polysaccharides, is routinely done by serotyping. Given the presence of 188 known O-antigens, this process is complex. The authors present a proof-of-concept planar microbead array for multiplexed O-serotyping. Ten clinically relevant E. coli serotypes associated with high risk for diarrhea in humans were examined (O26, O55, O78, O118, O124, O127, O128, O142, O145 and O157). Antisera were assigned to specific microbead populations, which can be differentiated by size and fluorescence color. Automatted image processing and data analysis were conducted by a microscopic interpretation platform. Homogenous antiserum coating of the microbeads was demonstrated by an intra-population CV that ranges from 3.3 to 6.3% and by an inter-population CV of 9.5%. Typical detections limits are in the range from 0.31 to 0.71 refMFI. Significantly elevated fluorescence signals revealed that E. coli of a certain serogroup bound specifically to microbeads with the matching antiserum (p < 0.001). In our perception, the method represents a viable diagnostic tool for automated multiplex serotyping of E. coli. It enables simultaneous and high-throughput screening for different O-antigens by a simple staining and binding protocol. Graphical abstractSchematic of a planar microbead array for the typing and classification of E. coli according to cell wall components. Based on coated fluorescent microbeads, multiplex O-serotyping of E. coli is accomplished via fluorescence imaging. Schematic of a planar microbead array for the typing and classification of E. coli according to cell wall components. Based on coated fluorescent microbeads, multiplex O-serotyping of E. coli is accomplished via fluorescence imaging. More... »

PAGES

1405-1415

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00604-017-2088-4

DOI

http://dx.doi.org/10.1007/s00604-017-2088-4

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1084022884


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universit\u00e4tsplatz 1, 01968, Senftenberg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liebsch", 
        "givenName": "Claudia", 
        "id": "sg:person.011724422511.50", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011724422511.50"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universit\u00e4tsplatz 1, 01968, Senftenberg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "R\u00f6diger", 
        "givenName": "Stefan", 
        "id": "sg:person.01201234036.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01201234036.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universit\u00e4tsplatz 1, 01968, Senftenberg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "B\u00f6hm", 
        "givenName": "Alexander", 
        "id": "sg:person.01002360621.71", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002360621.71"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universit\u00e4tsplatz 1, 01968, Senftenberg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nitschke", 
        "givenName": "J\u00f6rg", 
        "id": "sg:person.01050474021.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050474021.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universit\u00e4tsplatz 1, 01968, Senftenberg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Weinreich", 
        "givenName": "J\u00f6rg", 
        "id": "sg:person.01116607221.55", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01116607221.55"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Robert Koch Institute", 
          "id": "https://www.grid.ac/institutes/grid.13652.33", 
          "name": [
            "Branch Wernigerode, Robert Koch-Institute, Burgstra\u00dfe 37, 38855, Wernigerode, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fruth", 
        "givenName": "Angelika", 
        "id": "sg:person.01346372057.30", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346372057.30"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Generic Assays (Germany)", 
          "id": "https://www.grid.ac/institutes/grid.452429.c", 
          "name": [
            "Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universit\u00e4tsplatz 1, 01968, Senftenberg, Germany", 
            "GA Generic Assays GmbH, Ludwig-Erhard-Ring 3, 15827, Dahlewitz, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Roggenbuck", 
        "givenName": "Dirk", 
        "id": "sg:person.01207130524.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207130524.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Attomol GmbH, Schulweg 6, 03205, Lipten, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lehmann", 
        "givenName": "Werner", 
        "id": "sg:person.01153414517.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153414517.91"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "PolyAn GmbH, Rudolf-Baschant-Stra\u00dfe 2, 13086, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schedler", 
        "givenName": "Uwe", 
        "id": "sg:person.013537047361.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013537047361.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Carl-Thiem-Klinikum Cottbus", 
          "id": "https://www.grid.ac/institutes/grid.460801.b", 
          "name": [
            "Carl-Thiem-Klinikum Cottbus, Thiemstra\u00dfe 111, 03048, Cottbus, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Juretzek", 
        "givenName": "Thomas", 
        "id": "sg:person.01056612260.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01056612260.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universit\u00e4tsplatz 1, 01968, Senftenberg, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schierack", 
        "givenName": "Peter", 
        "id": "sg:person.01313347001.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313347001.01"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/s00604-010-0347-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004731737", 
          "https://doi.org/10.1007/s00604-010-0347-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00604-010-0347-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004731737", 
          "https://doi.org/10.1007/s00604-010-0347-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/dnares/dsu043", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005641656"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa1106482", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005742032"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.71.8.4941-4944.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006632777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1600-0463.2004.apm1120903.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007831237"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.01384-13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008268561"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.00854-09", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008842142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011876605", 
          "https://doi.org/10.1038/nrmicro818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro818", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011876605", 
          "https://doi.org/10.1038/nrmicro818"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s13317-016-0073-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013337312", 
          "https://doi.org/10.1007/s13317-016-0073-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s13317-016-0073-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013337312", 
          "https://doi.org/10.1007/s13317-016-0073-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00604-012-0824-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014722790", 
          "https://doi.org/10.1007/s00604-012-0824-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6976.2011.00298.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018544928"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00604-016-1769-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018614571", 
          "https://doi.org/10.1007/s00604-016-1769-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0580-9517(08)70447-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023449959"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/humu.28", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024786911"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00604-014-1243-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026558258", 
          "https://doi.org/10.1007/s00604-014-1243-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.aca.2015.12.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029087191"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/cyto.a.21009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030287270"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6976.2002.tb00597.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031299790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/cmr.00072-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031585780"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0140-6736(05)17745-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034724012"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2012/284740", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035535242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/32789", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036925108", 
          "https://doi.org/10.1038/32789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/32789", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036925108", 
          "https://doi.org/10.1038/32789"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0231(19960731)10:10<1227::aid-rcm659>3.0.co;2-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037902382"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/cclm-2015-0083", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038123222"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/10_2011_132", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038288567", 
          "https://doi.org/10.1007/10_2011_132"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00604-015-1623-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039971111", 
          "https://doi.org/10.1007/s00604-015-1623-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1371/journal.pone.0059242", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040752943"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.00217-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040925957"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cca.2014.04.024", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043012092"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1136/gutjnl-2014-307854", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047376404"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jcm.02215-09", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048998089"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac103277s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055001295"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac103277s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055001295"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/cmr.11.1.142", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083208763"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-05", 
    "datePublishedReg": "2017-05-01", 
    "description": "Typing and classification of Escherichia coli (E. coli) according to cell wall components, like polysaccharides, is routinely done by serotyping. Given the presence of 188 known O-antigens, this process is complex. The authors present a proof-of-concept planar microbead array for multiplexed O-serotyping. Ten clinically relevant E. coli serotypes associated with high risk for diarrhea in humans were examined (O26, O55, O78, O118, O124, O127, O128, O142, O145 and O157). Antisera were assigned to specific microbead populations, which can be differentiated by size and fluorescence color. Automatted image processing and data analysis were conducted by a microscopic interpretation platform. Homogenous antiserum coating of the microbeads was demonstrated by an intra-population CV that ranges from 3.3 to 6.3% and by an inter-population CV of 9.5%. Typical detections limits are in the range from 0.31 to 0.71 refMFI. Significantly elevated fluorescence signals revealed that E. coli of a certain serogroup bound specifically to microbeads with the matching antiserum (p < 0.001). In our perception, the method represents a viable diagnostic tool for automated multiplex serotyping of E. coli. It enables simultaneous and high-throughput screening for different O-antigens by a simple staining and binding protocol. Graphical abstractSchematic of a planar microbead array for the typing and classification of E. coli according to cell wall components. Based on coated fluorescent microbeads, multiplex O-serotyping of E. coli is accomplished via fluorescence imaging. Schematic of a planar microbead array for the typing and classification of E. coli according to cell wall components. Based on coated fluorescent microbeads, multiplex O-serotyping of E. coli is accomplished via fluorescence imaging.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00604-017-2088-4", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1009468", 
        "issn": [
          "0026-3672", 
          "1436-5073"
        ], 
        "name": "Microchimica Acta", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "184"
      }
    ], 
    "name": "Solid-phase microbead array for multiplex O-serotyping of Escherichia coli", 
    "pagination": "1405-1415", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "533a6c1d756ce63e41404cf9b7387cff8c34da2c60ea16bf16057c60c003f5f9"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00604-017-2088-4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1084022884"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00604-017-2088-4", 
      "https://app.dimensions.ai/details/publication/pub.1084022884"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:31", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000346_0000000346/records_99803_00000003.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs00604-017-2088-4"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00604-017-2088-4'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00604-017-2088-4'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00604-017-2088-4'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00604-017-2088-4'


 

This table displays all metadata directly associated to this object as RDF triples.

262 TRIPLES      21 PREDICATES      60 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00604-017-2088-4 schema:about anzsrc-for:06
2 anzsrc-for:0605
3 schema:author N635ae746e65f44b087ac573fbcfa9194
4 schema:citation sg:pub.10.1007/10_2011_132
5 sg:pub.10.1007/s00604-010-0347-8
6 sg:pub.10.1007/s00604-012-0824-3
7 sg:pub.10.1007/s00604-014-1243-4
8 sg:pub.10.1007/s00604-015-1623-4
9 sg:pub.10.1007/s00604-016-1769-8
10 sg:pub.10.1007/s13317-016-0073-2
11 sg:pub.10.1038/32789
12 sg:pub.10.1038/nrmicro818
13 https://doi.org/10.1002/(sici)1097-0231(19960731)10:10<1227::aid-rcm659>3.0.co;2-6
14 https://doi.org/10.1002/cyto.a.21009
15 https://doi.org/10.1002/humu.28
16 https://doi.org/10.1016/j.aca.2015.12.030
17 https://doi.org/10.1016/j.cca.2014.04.024
18 https://doi.org/10.1016/s0140-6736(05)17745-9
19 https://doi.org/10.1016/s0580-9517(08)70447-1
20 https://doi.org/10.1021/ac103277s
21 https://doi.org/10.1056/nejmoa1106482
22 https://doi.org/10.1093/dnares/dsu043
23 https://doi.org/10.1111/j.1574-6976.2002.tb00597.x
24 https://doi.org/10.1111/j.1574-6976.2011.00298.x
25 https://doi.org/10.1111/j.1600-0463.2004.apm1120903.x
26 https://doi.org/10.1128/aem.00217-12
27 https://doi.org/10.1128/aem.00854-09
28 https://doi.org/10.1128/aem.01384-13
29 https://doi.org/10.1128/aem.71.8.4941-4944.2005
30 https://doi.org/10.1128/cmr.00072-12
31 https://doi.org/10.1128/cmr.11.1.142
32 https://doi.org/10.1128/jcm.02215-09
33 https://doi.org/10.1136/gutjnl-2014-307854
34 https://doi.org/10.1155/2012/284740
35 https://doi.org/10.1371/journal.pone.0059242
36 https://doi.org/10.1515/cclm-2015-0083
37 schema:datePublished 2017-05
38 schema:datePublishedReg 2017-05-01
39 schema:description Typing and classification of Escherichia coli (E. coli) according to cell wall components, like polysaccharides, is routinely done by serotyping. Given the presence of 188 known O-antigens, this process is complex. The authors present a proof-of-concept planar microbead array for multiplexed O-serotyping. Ten clinically relevant E. coli serotypes associated with high risk for diarrhea in humans were examined (O26, O55, O78, O118, O124, O127, O128, O142, O145 and O157). Antisera were assigned to specific microbead populations, which can be differentiated by size and fluorescence color. Automatted image processing and data analysis were conducted by a microscopic interpretation platform. Homogenous antiserum coating of the microbeads was demonstrated by an intra-population CV that ranges from 3.3 to 6.3% and by an inter-population CV of 9.5%. Typical detections limits are in the range from 0.31 to 0.71 refMFI. Significantly elevated fluorescence signals revealed that E. coli of a certain serogroup bound specifically to microbeads with the matching antiserum (p < 0.001). In our perception, the method represents a viable diagnostic tool for automated multiplex serotyping of E. coli. It enables simultaneous and high-throughput screening for different O-antigens by a simple staining and binding protocol. Graphical abstractSchematic of a planar microbead array for the typing and classification of E. coli according to cell wall components. Based on coated fluorescent microbeads, multiplex O-serotyping of E. coli is accomplished via fluorescence imaging. Schematic of a planar microbead array for the typing and classification of E. coli according to cell wall components. Based on coated fluorescent microbeads, multiplex O-serotyping of E. coli is accomplished via fluorescence imaging.
40 schema:genre research_article
41 schema:inLanguage en
42 schema:isAccessibleForFree false
43 schema:isPartOf N19580450b635446f9761b79de07f2685
44 N465510161d5b41f0a3b930ad8d944ec8
45 sg:journal.1009468
46 schema:name Solid-phase microbead array for multiplex O-serotyping of Escherichia coli
47 schema:pagination 1405-1415
48 schema:productId N653a85dcb135439b8d44dfa8372b81ed
49 N6b24648cd7cd419a9cb8032a8c5c672f
50 N82a85caa8ec9473891b94f52a7eee283
51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084022884
52 https://doi.org/10.1007/s00604-017-2088-4
53 schema:sdDatePublished 2019-04-11T09:31
54 schema:sdLicense https://scigraph.springernature.com/explorer/license/
55 schema:sdPublisher N9281d006887848cc9a2de65950251876
56 schema:url https://link.springer.com/10.1007%2Fs00604-017-2088-4
57 sgo:license sg:explorer/license/
58 sgo:sdDataset articles
59 rdf:type schema:ScholarlyArticle
60 N16812afb12f34151bf74de712e50ecab schema:name Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, 01968, Senftenberg, Germany
61 rdf:type schema:Organization
62 N19580450b635446f9761b79de07f2685 schema:volumeNumber 184
63 rdf:type schema:PublicationVolume
64 N30cc0c2cf67747b29d0ab8545f20f4d8 rdf:first sg:person.01201234036.40
65 rdf:rest N888eb9541f9646cc81a43df725579848
66 N41aa292d3b24425aae3a16cb63d8adcf rdf:first sg:person.01056612260.84
67 rdf:rest N5dc94b87ca7e4d3893bcdb12ae948e00
68 N465510161d5b41f0a3b930ad8d944ec8 schema:issueNumber 5
69 rdf:type schema:PublicationIssue
70 N574657931e5647d9b1815f13a5b080e6 schema:name Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, 01968, Senftenberg, Germany
71 rdf:type schema:Organization
72 N5dc94b87ca7e4d3893bcdb12ae948e00 rdf:first sg:person.01313347001.01
73 rdf:rest rdf:nil
74 N635ae746e65f44b087ac573fbcfa9194 rdf:first sg:person.011724422511.50
75 rdf:rest N30cc0c2cf67747b29d0ab8545f20f4d8
76 N653a85dcb135439b8d44dfa8372b81ed schema:name doi
77 schema:value 10.1007/s00604-017-2088-4
78 rdf:type schema:PropertyValue
79 N6b24648cd7cd419a9cb8032a8c5c672f schema:name dimensions_id
80 schema:value pub.1084022884
81 rdf:type schema:PropertyValue
82 N6c6b11a635934c4689185a48cb727d4d schema:name Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, 01968, Senftenberg, Germany
83 rdf:type schema:Organization
84 N7d2e87ec69394fe48601ef0a57d9fd56 rdf:first sg:person.01153414517.91
85 rdf:rest N8ec2244b3a0a4ae8a756c128fc03e12f
86 N82a85caa8ec9473891b94f52a7eee283 schema:name readcube_id
87 schema:value 533a6c1d756ce63e41404cf9b7387cff8c34da2c60ea16bf16057c60c003f5f9
88 rdf:type schema:PropertyValue
89 N888eb9541f9646cc81a43df725579848 rdf:first sg:person.01002360621.71
90 rdf:rest N93f4e95e08ab4349afbbc77eeb1e217f
91 N8ec2244b3a0a4ae8a756c128fc03e12f rdf:first sg:person.013537047361.03
92 rdf:rest N41aa292d3b24425aae3a16cb63d8adcf
93 N8effeaa038224596a0385860fd575e8b schema:name Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, 01968, Senftenberg, Germany
94 rdf:type schema:Organization
95 N8f0c6336a81e41c0ab5cd315bbd5bcb4 schema:name Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, 01968, Senftenberg, Germany
96 rdf:type schema:Organization
97 N9281d006887848cc9a2de65950251876 schema:name Springer Nature - SN SciGraph project
98 rdf:type schema:Organization
99 N93f4e95e08ab4349afbbc77eeb1e217f rdf:first sg:person.01050474021.22
100 rdf:rest Ne9764af155d84d4f9afc8810c582e3d1
101 N9b21e1b1b7144d8f8df51f9688ad0ca5 schema:name Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, 01968, Senftenberg, Germany
102 rdf:type schema:Organization
103 N9e2c655b71c94903956bfd7e4a22dca4 rdf:first sg:person.01346372057.30
104 rdf:rest Nef50479e1cee4c61a8a722329045e831
105 Nb5e9529905ce49d0b0af3519b1067c1e schema:name Attomol GmbH, Schulweg 6, 03205, Lipten, Germany
106 rdf:type schema:Organization
107 Ndb8d90eb622741d79e4b21b48ccad618 schema:name PolyAn GmbH, Rudolf-Baschant-Straße 2, 13086, Berlin, Germany
108 rdf:type schema:Organization
109 Ne9764af155d84d4f9afc8810c582e3d1 rdf:first sg:person.01116607221.55
110 rdf:rest N9e2c655b71c94903956bfd7e4a22dca4
111 Nef50479e1cee4c61a8a722329045e831 rdf:first sg:person.01207130524.11
112 rdf:rest N7d2e87ec69394fe48601ef0a57d9fd56
113 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
114 schema:name Biological Sciences
115 rdf:type schema:DefinedTerm
116 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
117 schema:name Microbiology
118 rdf:type schema:DefinedTerm
119 sg:journal.1009468 schema:issn 0026-3672
120 1436-5073
121 schema:name Microchimica Acta
122 rdf:type schema:Periodical
123 sg:person.01002360621.71 schema:affiliation N8effeaa038224596a0385860fd575e8b
124 schema:familyName Böhm
125 schema:givenName Alexander
126 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002360621.71
127 rdf:type schema:Person
128 sg:person.01050474021.22 schema:affiliation N9b21e1b1b7144d8f8df51f9688ad0ca5
129 schema:familyName Nitschke
130 schema:givenName Jörg
131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01050474021.22
132 rdf:type schema:Person
133 sg:person.01056612260.84 schema:affiliation https://www.grid.ac/institutes/grid.460801.b
134 schema:familyName Juretzek
135 schema:givenName Thomas
136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01056612260.84
137 rdf:type schema:Person
138 sg:person.01116607221.55 schema:affiliation N6c6b11a635934c4689185a48cb727d4d
139 schema:familyName Weinreich
140 schema:givenName Jörg
141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01116607221.55
142 rdf:type schema:Person
143 sg:person.01153414517.91 schema:affiliation Nb5e9529905ce49d0b0af3519b1067c1e
144 schema:familyName Lehmann
145 schema:givenName Werner
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153414517.91
147 rdf:type schema:Person
148 sg:person.011724422511.50 schema:affiliation N574657931e5647d9b1815f13a5b080e6
149 schema:familyName Liebsch
150 schema:givenName Claudia
151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011724422511.50
152 rdf:type schema:Person
153 sg:person.01201234036.40 schema:affiliation N8f0c6336a81e41c0ab5cd315bbd5bcb4
154 schema:familyName Rödiger
155 schema:givenName Stefan
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01201234036.40
157 rdf:type schema:Person
158 sg:person.01207130524.11 schema:affiliation https://www.grid.ac/institutes/grid.452429.c
159 schema:familyName Roggenbuck
160 schema:givenName Dirk
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207130524.11
162 rdf:type schema:Person
163 sg:person.01313347001.01 schema:affiliation N16812afb12f34151bf74de712e50ecab
164 schema:familyName Schierack
165 schema:givenName Peter
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313347001.01
167 rdf:type schema:Person
168 sg:person.01346372057.30 schema:affiliation https://www.grid.ac/institutes/grid.13652.33
169 schema:familyName Fruth
170 schema:givenName Angelika
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01346372057.30
172 rdf:type schema:Person
173 sg:person.013537047361.03 schema:affiliation Ndb8d90eb622741d79e4b21b48ccad618
174 schema:familyName Schedler
175 schema:givenName Uwe
176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013537047361.03
177 rdf:type schema:Person
178 sg:pub.10.1007/10_2011_132 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038288567
179 https://doi.org/10.1007/10_2011_132
180 rdf:type schema:CreativeWork
181 sg:pub.10.1007/s00604-010-0347-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004731737
182 https://doi.org/10.1007/s00604-010-0347-8
183 rdf:type schema:CreativeWork
184 sg:pub.10.1007/s00604-012-0824-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014722790
185 https://doi.org/10.1007/s00604-012-0824-3
186 rdf:type schema:CreativeWork
187 sg:pub.10.1007/s00604-014-1243-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026558258
188 https://doi.org/10.1007/s00604-014-1243-4
189 rdf:type schema:CreativeWork
190 sg:pub.10.1007/s00604-015-1623-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039971111
191 https://doi.org/10.1007/s00604-015-1623-4
192 rdf:type schema:CreativeWork
193 sg:pub.10.1007/s00604-016-1769-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018614571
194 https://doi.org/10.1007/s00604-016-1769-8
195 rdf:type schema:CreativeWork
196 sg:pub.10.1007/s13317-016-0073-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013337312
197 https://doi.org/10.1007/s13317-016-0073-2
198 rdf:type schema:CreativeWork
199 sg:pub.10.1038/32789 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036925108
200 https://doi.org/10.1038/32789
201 rdf:type schema:CreativeWork
202 sg:pub.10.1038/nrmicro818 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011876605
203 https://doi.org/10.1038/nrmicro818
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1002/(sici)1097-0231(19960731)10:10<1227::aid-rcm659>3.0.co;2-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037902382
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1002/cyto.a.21009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030287270
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1002/humu.28 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024786911
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/j.aca.2015.12.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029087191
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/j.cca.2014.04.024 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043012092
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/s0140-6736(05)17745-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034724012
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1016/s0580-9517(08)70447-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023449959
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1021/ac103277s schema:sameAs https://app.dimensions.ai/details/publication/pub.1055001295
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1056/nejmoa1106482 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005742032
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1093/dnares/dsu043 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005641656
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1111/j.1574-6976.2002.tb00597.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1031299790
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1111/j.1574-6976.2011.00298.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1018544928
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1111/j.1600-0463.2004.apm1120903.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1007831237
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1128/aem.00217-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040925957
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1128/aem.00854-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008842142
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1128/aem.01384-13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008268561
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1128/aem.71.8.4941-4944.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006632777
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1128/cmr.00072-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031585780
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1128/cmr.11.1.142 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083208763
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1128/jcm.02215-09 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048998089
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1136/gutjnl-2014-307854 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047376404
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1155/2012/284740 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035535242
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1371/journal.pone.0059242 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040752943
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1515/cclm-2015-0083 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038123222
252 rdf:type schema:CreativeWork
253 https://www.grid.ac/institutes/grid.13652.33 schema:alternateName Robert Koch Institute
254 schema:name Branch Wernigerode, Robert Koch-Institute, Burgstraße 37, 38855, Wernigerode, Germany
255 rdf:type schema:Organization
256 https://www.grid.ac/institutes/grid.452429.c schema:alternateName Generic Assays (Germany)
257 schema:name GA Generic Assays GmbH, Ludwig-Erhard-Ring 3, 15827, Dahlewitz, Germany
258 Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, 01968, Senftenberg, Germany
259 rdf:type schema:Organization
260 https://www.grid.ac/institutes/grid.460801.b schema:alternateName Carl-Thiem-Klinikum Cottbus
261 schema:name Carl-Thiem-Klinikum Cottbus, Thiemstraße 111, 03048, Cottbus, Germany
262 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...