Differential Analysis of Rat Bone Marrow by Flow Cytometry View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-12

AUTHORS

A. Saad, M. Palm, S. Widell, S. Reiland

ABSTRACT

A procedure for rat bone marrow differential analysis using flow cytometry and commercially available monoclonal antibodies is described. The method uses a combination of the differential expression of leucocyte common antigen (CD45) on different cell lineages and the expression of transferrin receptor (CD71). This is coupled with the side scatter measurement (SSC) for morphological complexity and nucleic acid staining with LDS-751 for the separation of all nucleated cells from mature red blood cells. The relative number of erythroid, myeloid and lymphoid cells as well as the M:E ratio was generated by the flow cytometric method with acceptable reproducibility and a good agreement with microscopic analysis. There were no significant differences in mean values of the three cell populations between males and females or between bone marrow samples collected from left and right femurs. The flow cytometric analysis of bone marrow provides a semi-automated and rapid method that is suitable for the evaluation of rat bone marrow in the preclinical toxicity investigations. More... »

PAGES

97-101

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s005800070016

DOI

http://dx.doi.org/10.1007/s005800070016

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015476725


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