Ontology type: schema:ScholarlyArticle
2017-01-07
AUTHORSTanko N. Polycarp, Sabri M. Yusoff, Emikpe O. Benjamin, Shahrom M. Salisi, Siti-Khairani Bejo
ABSTRACTOxidative stress and its biomarkers are considered emerging fields in ruminant medicine. Data on this emerging field in dexamethasone-treated goats infected with Brucella melitensis is limited. This study aimed at evaluating biomarkers of oxidative stress in dexamethasone-treated non-pregnant Boer goats experimentally infected with B. melitensis. Eighteen healthy, non-pregnant goats were divided into three groups: A, B, and C of six animals each. Group A was treated with dexamethasone (2 mg/kg) for 7 days prior to inoculation with 107 CFU of B. melitensis ocularly. Group B was not treated but inoculated with similar dose of B. melitensis as in group A above while group C was administered normal saline. Blood samples were collected periodically through day 42 post inoculation (pi). Plasma cortisol, malondialdehyde (MDA), xanthine oxidase (XO), superoxide dismutase (SOD), glutathione (GSH), and serological responses (independent enzyme-linked immunosorbent assay and Rose Bengal Plate Test) were analyzed. Three animals from each group were sacrificed at days 21 and 42 pi and samples from the liver, kidney, and lungs were collected and processed for further analysis. Superoxide dismutase, GSH, and XO activities increased significantly (P < 0.001) in group A during dexamethasone treatment while MDA and cortisol decreased. Following Brucella inoculation, MDA, cortisol, and XO increased significantly while GSH and SOD decreased. In group B, MDA, cortisol, and XO activities increased with less significance as compared to group A. This study demonstrated that dexamethasone-induced stress distort the redox balance significantly and for a prolonged period, consequentially leading to enhanced infectiousness of B. melitensis. More... »
PAGES423-435
http://scigraph.springernature.com/pub.10.1007/s00580-016-2395-x
DOIhttp://dx.doi.org/10.1007/s00580-016-2395-x
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172 | ″ | ″ | Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia |
173 | ″ | schema:name | Department of Preclinical Science, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia |
174 | ″ | ″ | Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia |
175 | ″ | rdf:type | schema:Organization |
176 | grid-institutes:grid.9582.6 | schema:alternateName | Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria |
177 | ″ | schema:name | Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria |
178 | ″ | rdf:type | schema:Organization |