Copro-DNA test for diagnosis of canine echinococcosis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-09

AUTHORS

Hassan Borji, Abolghasem Naghibi, Farhad Jahangiri

ABSTRACT

Echinococcosis is one of the most important parasitic zoonotic diseases in the world. The life cycle of Echinococcus granulosus is dependent to the dog–sheep cycle and is actively transmitted in all pastoral regions where sheep, cattle and camels predominate. DNA approaches are now being used routinely for accurate identification of Echinococcus and Taenia species, subspecies and strains. In this study, faecal samples were collected from 50 stray dogs from Mashhad city in the northeast of Iran during June 2011. All samples were frozen at least for 1 week in −80°C. The embryophore layer of the eggs was broken by freezing–thawing method after egg concentration by the formalin-ether method. DNA was extracted using a DNA isolation kit (MBST, Germany/Iran) according to the manufacturer’s instructions. After PCR, by the primers expressed in the following it became, clear that about 20 % of stray dogs are infected with E. granulosus. In this study, we describe a modified method for DNA isolation from faeces for coprodiagnosis of Echinococcus spp. More... »

PAGES

909-912

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URI

http://scigraph.springernature.com/pub.10.1007/s00580-012-1498-2

DOI

http://dx.doi.org/10.1007/s00580-012-1498-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050899622


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