Droplet-based biochemical assay by magnetic wire manipulation between multiple droplets View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-02

AUTHORS

Mitsuhiro Shikida, Tatsuaki Sugito, Mina Okochi, Hiroyuki Honda

ABSTRACT

Reaction performance of a droplet-based biochemical assay technique that uses magnetic wires as sample carriers is evaluated. Wires 2.0 mm in length, 0.05 mm in width, and 0.02 mm in thickness are fabricated by chemical etching, introduced into a droplet immersed in oil, and manipulated by the magnetic force of a moving magnet. Alkaline-phosphatase as an enzyme is immobilized on the wire surfaces by applying Au and self-assembled monolayer coatings, and the method’s on-chip reaction performance is evaluated. The enzymatic reaction is found to increase linearly as the number of wires and the reaction time increase. Relatively high performance reproducibility for enzymatic reactions is obtained; on average, the reaction absorbance, standard deviation, and coefficient of variance are found to be respectively 1.14, 0.103, and 9.1 %. The conductivity change in a fused droplet is used to evaluate the absolute volume of liquid transferred with the extracted wires and a value of 0.33 μl is obtained. More... »

PAGES

315-323

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00542-013-1798-8

DOI

http://dx.doi.org/10.1007/s00542-013-1798-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020042319


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0306", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Physical Chemistry (incl. Structural)", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Nagoya University", 
          "id": "https://www.grid.ac/institutes/grid.27476.30", 
          "name": [
            "Center for Micro-Nano Mechatronics, Nagoya University, Nagoya, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shikida", 
        "givenName": "Mitsuhiro", 
        "id": "sg:person.013527570751.08", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013527570751.08"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagoya University", 
          "id": "https://www.grid.ac/institutes/grid.27476.30", 
          "name": [
            "Department of Micro-Nano Systems Engineering, Nagoya University, Nagoya, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sugito", 
        "givenName": "Tatsuaki", 
        "id": "sg:person.013555712572.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013555712572.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagoya University", 
          "id": "https://www.grid.ac/institutes/grid.27476.30", 
          "name": [
            "Department of Biotechnology, Nagoya University, Nagoya, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Okochi", 
        "givenName": "Mina", 
        "id": "sg:person.0740275451.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0740275451.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Nagoya University", 
          "id": "https://www.grid.ac/institutes/grid.27476.30", 
          "name": [
            "Department of Biotechnology, Nagoya University, Nagoya, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Honda", 
        "givenName": "Hiroyuki", 
        "id": "sg:person.016617716011.29", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016617716011.29"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/1522-2683(200110)22:18<3883::aid-elps3883>3.0.co;2-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007488830"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1088/0960-1317/15/2/r01", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008974561"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1088/0960-1317/3/4/002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011078471"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0924-4247(01)00734-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016638958"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.snb.2005.01.029", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028060407"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1088/0960-1317/18/3/035034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028438073"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1088/0960-1317/16/9/017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034757197"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbiosc.2009.07.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038069763"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0956-5663(01)00154-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042752430"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.snb.2007.10.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048818586"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b821508a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049828953"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b821508a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049828953"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/b107540n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052405617"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac049183o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054996433"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ac049183o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1054996433"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1063/1.1613038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057725777"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1109/28.703965", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1061143166"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1109/84.846697", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1061240745"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1109/jmems.2002.807467", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1061289750"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1109/jmems.2004.835775", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1061289942"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1109/memsys.2011.5734634", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1094609343"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-02", 
    "datePublishedReg": "2014-02-01", 
    "description": "Reaction performance of a droplet-based biochemical assay technique that uses magnetic wires as sample carriers is evaluated. Wires 2.0 mm in length, 0.05 mm in width, and 0.02 mm in thickness are fabricated by chemical etching, introduced into a droplet immersed in oil, and manipulated by the magnetic force of a moving magnet. Alkaline-phosphatase as an enzyme is immobilized on the wire surfaces by applying Au and self-assembled monolayer coatings, and the method\u2019s on-chip reaction performance is evaluated. The enzymatic reaction is found to increase linearly as the number of wires and the reaction time increase. Relatively high performance reproducibility for enzymatic reactions is obtained; on average, the reaction absorbance, standard deviation, and coefficient of variance are found to be respectively 1.14, 0.103, and 9.1 %. The conductivity change in a fused droplet is used to evaluate the absolute volume of liquid transferred with the extracted wires and a value of 0.33 \u03bcl is obtained.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00542-013-1798-8", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1294787", 
        "issn": [
          "0946-7076", 
          "1432-1858"
        ], 
        "name": "Microsystem Technologies", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "20"
      }
    ], 
    "name": "Droplet-based biochemical assay by magnetic wire manipulation between multiple droplets", 
    "pagination": "315-323", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "5f7e348190364c63c13ab7172e1f33d19ee430f5bb1ff420fa19a88b887c2b9c"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00542-013-1798-8"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1020042319"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00542-013-1798-8", 
      "https://app.dimensions.ai/details/publication/pub.1020042319"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000531.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00542-013-1798-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00542-013-1798-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00542-013-1798-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00542-013-1798-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00542-013-1798-8'


 

This table displays all metadata directly associated to this object as RDF triples.

141 TRIPLES      21 PREDICATES      46 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00542-013-1798-8 schema:about anzsrc-for:03
2 anzsrc-for:0306
3 schema:author N48bd700604b5475d8772bd2a6d4df45a
4 schema:citation https://doi.org/10.1002/1522-2683(200110)22:18<3883::aid-elps3883>3.0.co;2-4
5 https://doi.org/10.1016/j.jbiosc.2009.07.005
6 https://doi.org/10.1016/j.snb.2005.01.029
7 https://doi.org/10.1016/j.snb.2007.10.014
8 https://doi.org/10.1016/s0924-4247(01)00734-8
9 https://doi.org/10.1016/s0956-5663(01)00154-3
10 https://doi.org/10.1021/ac049183o
11 https://doi.org/10.1039/b107540n
12 https://doi.org/10.1039/b821508a
13 https://doi.org/10.1063/1.1613038
14 https://doi.org/10.1088/0960-1317/15/2/r01
15 https://doi.org/10.1088/0960-1317/16/9/017
16 https://doi.org/10.1088/0960-1317/18/3/035034
17 https://doi.org/10.1088/0960-1317/3/4/002
18 https://doi.org/10.1109/28.703965
19 https://doi.org/10.1109/84.846697
20 https://doi.org/10.1109/jmems.2002.807467
21 https://doi.org/10.1109/jmems.2004.835775
22 https://doi.org/10.1109/memsys.2011.5734634
23 schema:datePublished 2014-02
24 schema:datePublishedReg 2014-02-01
25 schema:description Reaction performance of a droplet-based biochemical assay technique that uses magnetic wires as sample carriers is evaluated. Wires 2.0 mm in length, 0.05 mm in width, and 0.02 mm in thickness are fabricated by chemical etching, introduced into a droplet immersed in oil, and manipulated by the magnetic force of a moving magnet. Alkaline-phosphatase as an enzyme is immobilized on the wire surfaces by applying Au and self-assembled monolayer coatings, and the method’s on-chip reaction performance is evaluated. The enzymatic reaction is found to increase linearly as the number of wires and the reaction time increase. Relatively high performance reproducibility for enzymatic reactions is obtained; on average, the reaction absorbance, standard deviation, and coefficient of variance are found to be respectively 1.14, 0.103, and 9.1 %. The conductivity change in a fused droplet is used to evaluate the absolute volume of liquid transferred with the extracted wires and a value of 0.33 μl is obtained.
26 schema:genre research_article
27 schema:inLanguage en
28 schema:isAccessibleForFree false
29 schema:isPartOf N1cd0f71f06224864ae5997901c6cbe07
30 N4c63cfa7d6674ed997227dc1c5a55409
31 sg:journal.1294787
32 schema:name Droplet-based biochemical assay by magnetic wire manipulation between multiple droplets
33 schema:pagination 315-323
34 schema:productId N02ec1f1dc2ca495a826bdf936e6f0805
35 N0fdf8898ef8044879ea0583382fff8d6
36 Ndde75e5037614f91b7535a4c8e986f6b
37 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020042319
38 https://doi.org/10.1007/s00542-013-1798-8
39 schema:sdDatePublished 2019-04-10T15:55
40 schema:sdLicense https://scigraph.springernature.com/explorer/license/
41 schema:sdPublisher N44059e6cc76549cf89f87abb9fe6ab43
42 schema:url http://link.springer.com/10.1007%2Fs00542-013-1798-8
43 sgo:license sg:explorer/license/
44 sgo:sdDataset articles
45 rdf:type schema:ScholarlyArticle
46 N02ec1f1dc2ca495a826bdf936e6f0805 schema:name readcube_id
47 schema:value 5f7e348190364c63c13ab7172e1f33d19ee430f5bb1ff420fa19a88b887c2b9c
48 rdf:type schema:PropertyValue
49 N0fdf8898ef8044879ea0583382fff8d6 schema:name doi
50 schema:value 10.1007/s00542-013-1798-8
51 rdf:type schema:PropertyValue
52 N178e04b94827425aa3b5041ba89a93d5 rdf:first sg:person.0740275451.42
53 rdf:rest N843f45e78d394538864fb72cdda7f7e4
54 N1cd0f71f06224864ae5997901c6cbe07 schema:issueNumber 2
55 rdf:type schema:PublicationIssue
56 N44059e6cc76549cf89f87abb9fe6ab43 schema:name Springer Nature - SN SciGraph project
57 rdf:type schema:Organization
58 N48bd700604b5475d8772bd2a6d4df45a rdf:first sg:person.013527570751.08
59 rdf:rest Nc3dc440d473044e0b2b413660c89904e
60 N4c63cfa7d6674ed997227dc1c5a55409 schema:volumeNumber 20
61 rdf:type schema:PublicationVolume
62 N843f45e78d394538864fb72cdda7f7e4 rdf:first sg:person.016617716011.29
63 rdf:rest rdf:nil
64 Nc3dc440d473044e0b2b413660c89904e rdf:first sg:person.013555712572.03
65 rdf:rest N178e04b94827425aa3b5041ba89a93d5
66 Ndde75e5037614f91b7535a4c8e986f6b schema:name dimensions_id
67 schema:value pub.1020042319
68 rdf:type schema:PropertyValue
69 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
70 schema:name Chemical Sciences
71 rdf:type schema:DefinedTerm
72 anzsrc-for:0306 schema:inDefinedTermSet anzsrc-for:
73 schema:name Physical Chemistry (incl. Structural)
74 rdf:type schema:DefinedTerm
75 sg:journal.1294787 schema:issn 0946-7076
76 1432-1858
77 schema:name Microsystem Technologies
78 rdf:type schema:Periodical
79 sg:person.013527570751.08 schema:affiliation https://www.grid.ac/institutes/grid.27476.30
80 schema:familyName Shikida
81 schema:givenName Mitsuhiro
82 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013527570751.08
83 rdf:type schema:Person
84 sg:person.013555712572.03 schema:affiliation https://www.grid.ac/institutes/grid.27476.30
85 schema:familyName Sugito
86 schema:givenName Tatsuaki
87 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013555712572.03
88 rdf:type schema:Person
89 sg:person.016617716011.29 schema:affiliation https://www.grid.ac/institutes/grid.27476.30
90 schema:familyName Honda
91 schema:givenName Hiroyuki
92 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016617716011.29
93 rdf:type schema:Person
94 sg:person.0740275451.42 schema:affiliation https://www.grid.ac/institutes/grid.27476.30
95 schema:familyName Okochi
96 schema:givenName Mina
97 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0740275451.42
98 rdf:type schema:Person
99 https://doi.org/10.1002/1522-2683(200110)22:18<3883::aid-elps3883>3.0.co;2-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007488830
100 rdf:type schema:CreativeWork
101 https://doi.org/10.1016/j.jbiosc.2009.07.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038069763
102 rdf:type schema:CreativeWork
103 https://doi.org/10.1016/j.snb.2005.01.029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028060407
104 rdf:type schema:CreativeWork
105 https://doi.org/10.1016/j.snb.2007.10.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048818586
106 rdf:type schema:CreativeWork
107 https://doi.org/10.1016/s0924-4247(01)00734-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016638958
108 rdf:type schema:CreativeWork
109 https://doi.org/10.1016/s0956-5663(01)00154-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042752430
110 rdf:type schema:CreativeWork
111 https://doi.org/10.1021/ac049183o schema:sameAs https://app.dimensions.ai/details/publication/pub.1054996433
112 rdf:type schema:CreativeWork
113 https://doi.org/10.1039/b107540n schema:sameAs https://app.dimensions.ai/details/publication/pub.1052405617
114 rdf:type schema:CreativeWork
115 https://doi.org/10.1039/b821508a schema:sameAs https://app.dimensions.ai/details/publication/pub.1049828953
116 rdf:type schema:CreativeWork
117 https://doi.org/10.1063/1.1613038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057725777
118 rdf:type schema:CreativeWork
119 https://doi.org/10.1088/0960-1317/15/2/r01 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008974561
120 rdf:type schema:CreativeWork
121 https://doi.org/10.1088/0960-1317/16/9/017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034757197
122 rdf:type schema:CreativeWork
123 https://doi.org/10.1088/0960-1317/18/3/035034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028438073
124 rdf:type schema:CreativeWork
125 https://doi.org/10.1088/0960-1317/3/4/002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011078471
126 rdf:type schema:CreativeWork
127 https://doi.org/10.1109/28.703965 schema:sameAs https://app.dimensions.ai/details/publication/pub.1061143166
128 rdf:type schema:CreativeWork
129 https://doi.org/10.1109/84.846697 schema:sameAs https://app.dimensions.ai/details/publication/pub.1061240745
130 rdf:type schema:CreativeWork
131 https://doi.org/10.1109/jmems.2002.807467 schema:sameAs https://app.dimensions.ai/details/publication/pub.1061289750
132 rdf:type schema:CreativeWork
133 https://doi.org/10.1109/jmems.2004.835775 schema:sameAs https://app.dimensions.ai/details/publication/pub.1061289942
134 rdf:type schema:CreativeWork
135 https://doi.org/10.1109/memsys.2011.5734634 schema:sameAs https://app.dimensions.ai/details/publication/pub.1094609343
136 rdf:type schema:CreativeWork
137 https://www.grid.ac/institutes/grid.27476.30 schema:alternateName Nagoya University
138 schema:name Center for Micro-Nano Mechatronics, Nagoya University, Nagoya, Japan
139 Department of Biotechnology, Nagoya University, Nagoya, Japan
140 Department of Micro-Nano Systems Engineering, Nagoya University, Nagoya, Japan
141 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...